PMID- 26677678
OWN - NLM
STAT- MEDLINE
DCOM- 20160915
LR  - 20181202
IS  - 1003-5370 (Print)
IS  - 1003-5370 (Linking)
VI  - 35
IP  - 10
DP  - 2015 Oct
TI  - [Prevention and Treatment of Atherosclerosis by Three Different Chinese Medical 
      Compounds: a Mechanism Study].
PG  - 1244-8
AB  - OBJECTIVE: To study the effect of Buyang Huanwu Decoction (BHD), Xuefu Zhuyu 
      Decoction (XZD), and Sijunzi Decoction (SD) contained serums on expressions of 
      Toll-like receptor 4 (TLR4)/nuclear factor (NF)-kappaB signals, lectin-like oxidized 
      low-density lipoprotein receptor-1 (LOX-1), tumor necrosis factor-alpha (TNF-alpha), 
      vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 
      (ICAM-1), and to explore possible anti-atherosclerotic mechanisms. METHODS: 
      Twenty New Zealand rabbits were divided into 4 groups at random, i.e., the normal 
      control group, the BHD group (6.7 g/kg), the XZD group (3.6 g/kg), and the SD 
      group (1.6 g/kg), 5 in each group. All medication lasted for 7 successive days. 
      Two h after the final medication, about 50 mL blood was withdrawn from rabbit 
      heart for preparing serums. Human umbilical vein endothelial cell ECV304 were 
      cultured in vitro for 18 h and randomly divided into the blank control group, the 
      model group, the Western medicine (WM) control group, the BHD group, the XZD 
      group, and the SD group at random. ECV304, except in the blank control group, 
      were stimulated with lipopolysaccharide (LPS) for 2 h. Those in the WM control 
      group and CM groups were treated respectively with corresponding CM contained 
      serum for 24 h. Finally gene and protein expressions of TLR4, myeloid 
      differentiation factor 88 (MyD88), tumor necrosis factor receptor-associated 
      factor-6 (TRAF-6), NF-kappaB, LOX-1, TNF-alpha, ICAM-1, and VCAM-1 were detected by 
      fluorescent quantitative PCR and Western blot. RESULTS: Compared with the blank 
      control group, mRNA expressions of TLR4, MyD88, TRAF-6, NF-KB, LOX-1 , TNF-cx, 
      ICAM-1, and VCAM-1 increased significantly; protein expressions of TLR4, NF-kappaB, 
      LOX-1, TNF-alpha, ICAM-1, and VCAM-1 also increased significantly in the model group 
      (P < 0.01). Compared with the model group, mRNA and protein expressions of each 
      index could be significantly inhibited in the BHD group, the XZD group, and the 
      WM control group (P < 0.05). Besides, mRNA and protein expressions of each index 
      could be significantly elevated more in the BHD group and the XZD group than in 
      the WM control group (P < 0.05). No statistical difference existed in each index 
      between the SD group and the rest groups (P > 0.05). CONCLUSIONS: The mechanism 
      of BHD and XZD for fighting against atherosclerosis might be associated with 
      inhibiting TLR4/NF-kappaB signal transduction pathway and expressions of its 
      downstream inflammatory factors such as LOX-1, TNF-alpha, ICAM-1, and VCAM-1. But SD 
      showed no associated effect on atherosclerosis.
FAU - Jiang, Hua
AU  - Jiang H
FAU - Jiang, Yu-ji
AU  - Jiang YJ
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Xi Yi Jie He Za Zhi
JT  - Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese 
      journal of integrated traditional and Western medicine
JID - 9211576
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (NF-kappa B)
RN  - 0 (Scavenger Receptors, Class E)
RN  - 0 (TNF Receptor-Associated Factor 6)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 0 (Xue-Fu-Zhu-Yu decoction)
RN  - 0 (buyang huanwu)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Animals
MH  - Atherosclerosis/*drug therapy
MH  - Drugs, Chinese Herbal/*pharmacology/therapeutic use
MH  - Endothelial Cells
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Lipopolysaccharides
MH  - Myeloid Differentiation Factor 88/metabolism
MH  - NF-kappa B/metabolism
MH  - Rabbits
MH  - Scavenger Receptors, Class E
MH  - Signal Transduction
MH  - TNF Receptor-Associated Factor 6/metabolism
MH  - Toll-Like Receptor 4/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Umbilical Veins
MH  - Vascular Cell Adhesion Molecule-1/metabolism
EDAT- 2015/12/19 06:00
MHDA- 2016/09/16 06:00
CRDT- 2015/12/19 06:00
PHST- 2015/12/19 06:00 [entrez]
PHST- 2015/12/19 06:00 [pubmed]
PHST- 2016/09/16 06:00 [medline]
PST - ppublish
SO  - Zhongguo Zhong Xi Yi Jie He Za Zhi. 2015 Oct;35(10):1244-8.