PMID- 26679802
OWN - NLM
STAT- MEDLINE
DCOM- 20160824
LR  - 20220331
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Linking)
VI  - 103
DP  - 2015 Dec
TI  - Immunoregulatory signal FoxP3, cytokine gene expression and IFN-gamma cell 
      responsiveness upon porcine reproductive and respiratory syndrome virus (PRRSV) 
      natural infection.
PG  - 96-102
LID - S0034-5288(15)30067-9 [pii]
LID - 10.1016/j.rvsc.2015.09.018 [doi]
AB  - The study aims at evaluating gene expression of pro-inflammatory (IL-1beta, IL-8, 
      TNF-alpha), pro-immune (IFN-gamma), anti-inflammatory (IL-10) cytokines and of the 
      immunoregulatory signal FoxP3 in association with PRRSV-specific IFN-gamma secreting 
      cell (SC) responsiveness upon PRRSV natural infection. Forty PRRSV-negative pigs 
      were assigned to two groups: 20 pigs were vaccinated at 3 weeks of age (weaning) 
      against PRRSV (V-PRRSV) with a modified live virus vaccine (MLV) and 20 pigs were 
      kept non-vaccinated (NV) as controls. Blood samples were collected at 3 
      (vaccination), 6, 8, 10, 12, 14, and 16 weeks of age. Natural infection occurred 
      from 8 weeks of age onward in both groups and viremia lasted 8 weeks. In the 
      early phase of infection, pro-inflammatory cytokines (IL-1beta, IL-8, TNF-alpha) showed 
      a delayed increase concomitant with the peak of viremia in both groups. In both 
      groups, IL-10 peaked at 12 weeks in association with the increase of 
      pro-inflammatory cytokines. Conversely, in vaccinated pigs (V-PRRSV), IFN-gamma 
      showed higher gene expression during the early phase of infection and a more 
      intense secreting cell (SC) response in the late phase. Differently, gene 
      expression of the transcription factor FoxP3, expressed by T regulatory 
      lymphocytes (Tregs), increased significantly in controls only and was associated 
      with the rise of the viral load. Moreover, FoxP3 levels remained significantly 
      higher during the late phase of infection and paralleled with lower levels of 
      IFN-gamma SC detected by ELISPOT. The expression/production of immunoregulatory 
      signals involved in Treg activation could be a promising marker to study the 
      immunobiology of PRRSV infection.
FAU - Ferrarini, Giulia
AU  - Ferrarini G
AD  - Department of Veterinary Sciences, University of Parma, Via del Taglio, 10, 43126 
      Parma, Italy. Electronic address: giulia.ferrarini@studenti.unipr.it.
FAU - Borghetti, Paolo
AU  - Borghetti P
AD  - Department of Veterinary Sciences, University of Parma, Via del Taglio, 10, 43126 
      Parma, Italy. Electronic address: paolo.borghetti@unipr.it.
FAU - De Angelis, Elena
AU  - De Angelis E
AD  - Department of Veterinary Sciences, University of Parma, Via del Taglio, 10, 43126 
      Parma, Italy. Electronic address: elena.deangelis@unipr.it.
FAU - Ferrari, Luca
AU  - Ferrari L
AD  - Department of Veterinary Sciences, University of Parma, Via del Taglio, 10, 43126 
      Parma, Italy. Electronic address: luca.ferrari@unipr.it.
FAU - Canelli, Elena
AU  - Canelli E
AD  - Department of Veterinary Sciences, University of Parma, Via del Taglio, 10, 43126 
      Parma, Italy. Electronic address: elena.canelli@libero.it.
FAU - Catella, Alessia
AU  - Catella A
AD  - Department of Veterinary Sciences, University of Parma, Via del Taglio, 10, 43126 
      Parma, Italy. Electronic address: alessia.catella@alice.it.
FAU - Di Lecce, Rosanna
AU  - Di Lecce R
AD  - Department of Veterinary Sciences, University of Parma, Via del Taglio, 10, 43126 
      Parma, Italy. Electronic address: rosanna.dilecce@unipr.it.
FAU - Martelli, Paolo
AU  - Martelli P
AD  - Department of Veterinary Sciences, University of Parma, Via del Taglio, 10, 43126 
      Parma, Italy. Electronic address: paolo.martelli@unipr.it.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150926
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (Cytokines)
RN  - 0 (Forkhead Transcription Factors)
SB  - IM
MH  - Animals
MH  - Cytokines/*genetics/metabolism
MH  - Female
MH  - Forkhead Transcription Factors/*genetics/metabolism
MH  - *Gene Expression Regulation
MH  - Leukocytes, Mononuclear/metabolism/virology
MH  - Porcine Reproductive and Respiratory Syndrome/*genetics/immunology/metabolism
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine
OTO - NOTNLM
OT  - Cytokines
OT  - Forkhead box P3 (FoxP3)
OT  - Interferon-gamma (IFN-gamma) secreting cell (SC) responsiveness
OT  - Pig
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
EDAT- 2015/12/19 06:00
MHDA- 2016/08/25 06:00
CRDT- 2015/12/19 06:00
PHST- 2015/08/04 00:00 [received]
PHST- 2015/09/18 00:00 [revised]
PHST- 2015/09/23 00:00 [accepted]
PHST- 2015/12/19 06:00 [entrez]
PHST- 2015/12/19 06:00 [pubmed]
PHST- 2016/08/25 06:00 [medline]
AID - S0034-5288(15)30067-9 [pii]
AID - 10.1016/j.rvsc.2015.09.018 [doi]
PST - ppublish
SO  - Res Vet Sci. 2015 Dec;103:96-102. doi: 10.1016/j.rvsc.2015.09.018. Epub 2015 Sep 
      26.