PMID- 26680554
OWN - NLM
STAT- MEDLINE
DCOM- 20161006
LR  - 20181113
IS  - 1471-2164 (Electronic)
IS  - 1471-2164 (Linking)
VI  - 16 Suppl 12
IP  - Suppl 12
DP  - 2015
TI  - Investigation of microRNAs in mouse macrophage responses to 
      lipopolysaccharide-stimulation by combining gene expression with microRNA-target 
      information.
PG  - S13
LID - 10.1186/1471-2164-16-S12-S13 [doi]
AB  - BACKGROUND: Toll-like receptors, which stimulated by pathogen-associated 
      molecular patterns such as lipopolysaccharides (LPS), induces the releasing of 
      many kinds of proinflammatory cytokines to activate subsequent immune responses. 
      Plenty of studies have also indicated the importance of TLR-signalling on the 
      avoidance of excessive inflammation, tissue repairing and the return to 
      homeostasis after infection and tissue injury. The significance of TLR-signalling 
      attracts many attentions on the regulatory mechanisms since several years ago. 
      However, as newly discovered regulators, how and how many different microRNAs 
      (miRNAs) regulate TLR-signalling pathway are still unclear. RESULTS: By 
      integrating several microarray datasets and miRNA-target information datasets, we 
      identified 431 miRNAs and 498 differentially expressed target genes in bone 
      marrow-derived macrophages (BMDMs) with LPS-stimulation. Cooperative miRNA 
      network were constructed by calculating targets overlap scores, and a sub-network 
      finding algorithm was used to identify cooperative miRNA modules. Finally, 17 and 
      8 modules are identified in the cooperative miRNA networks composed of miRNAs 
      up-regulate and down-regulate genes, respectively. CONCLUSIONS: We used gene 
      expression data of mouse macrophage stimulated by LPS and miRNA-target 
      information to infer the regulatory mechanism of miRNAs on LPS-induced signalling 
      pathway. Also, our results suggest that miRNAs can be important regulators of 
      LPS-induced innate immune response in BMDMs.
FAU - Chiu, Chia-Chun
AU  - Chiu CC
FAU - Wu, Wei-Sheng
AU  - Wu WS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151209
PL  - England
TA  - BMC Genomics
JT  - BMC genomics
JID - 100965258
RN  - 0 (Lipopolysaccharides)
RN  - 0 (MicroRNAs)
RN  - 0 (Toll-Like Receptors)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Computational Biology/methods
MH  - Databases, Genetic
MH  - Gene Expression Profiling/*methods
MH  - Gene Expression Regulation/drug effects
MH  - *Gene Regulatory Networks/drug effects
MH  - Lipopolysaccharides/*pharmacology
MH  - Macrophages/cytology/*drug effects
MH  - Mice
MH  - MicroRNAs/*genetics
MH  - Signal Transduction/drug effects
MH  - Toll-Like Receptors/genetics
PMC - PMC4682375
EDAT- 2015/12/19 06:00
MHDA- 2016/10/08 06:00
PMCR- 2015/12/09
CRDT- 2015/12/19 06:00
PHST- 2015/12/19 06:00 [entrez]
PHST- 2015/12/19 06:00 [pubmed]
PHST- 2016/10/08 06:00 [medline]
PHST- 2015/12/09 00:00 [pmc-release]
AID - 1471-2164-16-S12-S13 [pii]
AID - 10.1186/1471-2164-16-S12-S13 [doi]
PST - ppublish
SO  - BMC Genomics. 2015;16 Suppl 12(Suppl 12):S13. doi: 10.1186/1471-2164-16-S12-S13. 
      Epub 2015 Dec 9.