PMID- 26680722
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160127
LR  - 20191113
IS  - 1580-3155 (Electronic)
IS  - 1318-0207 (Linking)
VI  - 62
IP  - 4
DP  - 2015
TI  - Effect of Solid State Fermentation Medium Optimization on Pleurotus ostreatus 
      Laccase Production.
PG  - 932-9
AB  - The objective of this work was to increase laccase production by Pleurotus 
      ostreatus PLAB through culture medium optimization using solid state culture 
      conditions. Increased laccase activity was obtained through design of experiments 
      (DOE) using the Taguchi orthogonal array (OA). Seven factors, viz. 
      lignocellulose, glucose, yeast extract, peptone, KH(2)PO(4), MgSO(4) 7H(2)O and 
      MnSO(4) H(2)O at three levels and pH at two levels. OA layout of L18 (2(1) x 
      3(7)) was selected for the proposed experimental design using Minitab 17 
      software. Data analysis showed that lignocellulose (20 %) and glucose (10 g L1) 
      had positive effect, whereas KH(2)PO(4), MgSO(4)∙7H(2)O and MnSO(4)∙H(2)O did not 
      have significant effect on laccase production. Taguchi OA analysis showed that pH 
      6, lignocellulose 20 %, glucose 10 g L(-1), yeast extract 6 g L(-1), peptone 15 g 
      L(-1), KH(2)PO(4) 3 g L1, MgSO(4)∙7H(2)O 0.5 g L(-1) and MnSO(4)∙H(2)O 0.1 g L-1 
      were the optimal conditions to maximize laccase production. The model predicted a 
      30.37 U g(-1) dry wt., which agreed with the experimentally obtained laccase 
      activity 29.15 U g(-1) dry wt. at optimal conditions.
FAU - Belsak-Sel, Natasa
AU  - Belsak-Sel N
FAU - Gregori, Andrej
AU  - Gregori A
FAU - Leitgeb, Maja
AU  - Leitgeb M
FAU - Klinara, Dusan
AU  - Klinara D
FAU - Celan, Stefan
AU  - Celan S
LA  - eng
PT  - Journal Article
PL  - Slovenia
TA  - Acta Chim Slov
JT  - Acta chimica Slovenica
JID - 101247110
EDAT- 2015/12/19 06:00
MHDA- 2015/12/19 06:01
CRDT- 2015/12/19 06:00
PHST- 2015/12/19 06:00 [entrez]
PHST- 2015/12/19 06:00 [pubmed]
PHST- 2015/12/19 06:01 [medline]
AID - Belsak-Sel-2015-4 [pii]
AID - 10.17344/acsi.2015.1764 [doi]
PST - ppublish
SO  - Acta Chim Slov. 2015;62(4):932-9. doi: 10.17344/acsi.2015.1764.