PMID- 26681597
OWN - NLM
STAT- MEDLINE
DCOM- 20161101
LR  - 20240324
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 5
DP  - 2015 Dec 18
TI  - Functional characterization of NADPH-cytochrome P450 reductase from Bactrocera 
      dorsalis: Possible involvement in susceptibility to malathion.
PG  - 18394
LID - 10.1038/srep18394 [doi]
LID - 18394
AB  - NADPH cytochrome P450 reductase (CPR) is essential for cytochrome P450 catalysis, 
      which is important in the detoxification and activation of xenobiotics. In this 
      study, two transcripts of Bactrocera dorsalis CPR (BdCPR) were cloned, and the 
      deduced amino-acid sequence had an N-terminus membrane anchor for BdCPR-X1 and 
      three conserved binding domains (FMN, FAD, and NADP), as well as an FAD binding 
      motif and catalytic residues for both BdCPR-X1 and BdCPR-X2. BdCPR-X1 was 
      detected to have the high expression levels in adults and in Malpighian tubules, 
      fat bodies, and midguts of adults, but BdCPR-X2 expressed lowly in B. dorsalis. 
      The levels of BdCPRs were similar in malathion-resistant strain compared to 
      susceptible strain. However, injecting adults with double-stranded RNA against 
      BdCPR significantly reduced the transcript levels of the mRNA, and knockdown of 
      BdCPR increased adult susceptibility to malathion. Expressing complete BdCPR-X1 
      cDNA in Sf9 cells resulted in high activity determined by cytochrome c reduction 
      and these cells had higher viability after exposure to malathion than control. 
      The results suggest that BdCPR could affect the susceptibility of B. dorsalis to 
      malathion and eukaryotic expression of BdCPR would lay a solid foundation for 
      further investigation of P450 in B. dorsalis.
FAU - Huang, Yong
AU  - Huang Y
AD  - Key Laboratory of Entomology and Pest Control Engineering, College of Plant 
      Protection, Southwest University, Chongqing 400716, P. R. China.
FAU - Lu, Xue-Ping
AU  - Lu XP
AD  - Key Laboratory of Entomology and Pest Control Engineering, College of Plant 
      Protection, Southwest University, Chongqing 400716, P. R. China.
FAU - Wang, Luo-Luo
AU  - Wang LL
AD  - Key Laboratory of Entomology and Pest Control Engineering, College of Plant 
      Protection, Southwest University, Chongqing 400716, P. R. China.
FAU - Wei, Dong
AU  - Wei D
AD  - Key Laboratory of Entomology and Pest Control Engineering, College of Plant 
      Protection, Southwest University, Chongqing 400716, P. R. China.
FAU - Feng, Zi-Jiao
AU  - Feng ZJ
AD  - Key Laboratory of Entomology and Pest Control Engineering, College of Plant 
      Protection, Southwest University, Chongqing 400716, P. R. China.
FAU - Zhang, Qi
AU  - Zhang Q
AD  - Key Laboratory of Entomology and Pest Control Engineering, College of Plant 
      Protection, Southwest University, Chongqing 400716, P. R. China.
FAU - Xiao, Lin-Fan
AU  - Xiao LF
AD  - Key Laboratory of Entomology and Pest Control Engineering, College of Plant 
      Protection, Southwest University, Chongqing 400716, P. R. China.
FAU - Dou, Wei
AU  - Dou W
AD  - Key Laboratory of Entomology and Pest Control Engineering, College of Plant 
      Protection, Southwest University, Chongqing 400716, P. R. China.
FAU - Wang, Jin-Jun
AU  - Wang JJ
AD  - Key Laboratory of Entomology and Pest Control Engineering, College of Plant 
      Protection, Southwest University, Chongqing 400716, P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151218
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Insecticides)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Double-Stranded)
RN  - 0 (RNA, Messenger)
RN  - 9007-43-6 (Cytochromes c)
RN  - EC 1.6.2.4 (NADPH-Ferrihemoprotein Reductase)
RN  - U5N7SU872W (Malathion)
SB  - IM
MH  - Animals
MH  - Cloning, Molecular
MH  - Cytochromes c/metabolism
MH  - Female
MH  - Insecticide Resistance/*genetics
MH  - Insecticides/*toxicity
MH  - Malathion/*toxicity
MH  - Male
MH  - NADPH-Ferrihemoprotein Reductase/antagonists & inhibitors/genetics/*metabolism
MH  - Phylogeny
MH  - Protein Isoforms/classification/genetics/metabolism
MH  - RNA Interference
MH  - RNA, Double-Stranded/metabolism
MH  - RNA, Messenger/metabolism
MH  - Sequence Analysis, DNA
MH  - Sf9 Cells
MH  - Spodoptera
MH  - Tephritidae/*drug effects/*enzymology
MH  - Transcriptome
PMC - PMC4683403
EDAT- 2015/12/19 06:00
MHDA- 2016/11/02 06:00
PMCR- 2015/12/18
CRDT- 2015/12/19 06:00
PHST- 2015/08/14 00:00 [received]
PHST- 2015/11/17 00:00 [accepted]
PHST- 2015/12/19 06:00 [entrez]
PHST- 2015/12/19 06:00 [pubmed]
PHST- 2016/11/02 06:00 [medline]
PHST- 2015/12/18 00:00 [pmc-release]
AID - srep18394 [pii]
AID - 10.1038/srep18394 [doi]
PST - epublish
SO  - Sci Rep. 2015 Dec 18;5:18394. doi: 10.1038/srep18394.