PMID- 26682926
OWN - NLM
STAT- MEDLINE
DCOM- 20160527
LR  - 20160117
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 469
IP  - 3
DP  - 2016 Jan 15
TI  - Cathepsin D in pancreatic acinar cells is implicated in cathepsin B and L 
      degradation, but not in autophagic activity.
PG  - 405-11
LID - S0006-291X(15)31007-X [pii]
LID - 10.1016/j.bbrc.2015.12.002 [doi]
AB  - Cathepsin D (CD) is the major lysosomal aspartic protease and is widely 
      distributed in the cells of various mammalian tissues. CD participates in various 
      physiological events such as regulation of programmed cell death, activation of 
      enzymatic precursors, and metabolic degradation of intracellular proteins through 
      macroautophagy. To investigate the role of CD in pancreatic acinar cells, which 
      constitute the exocrine pancreas, we generated and examined mice specifically 
      deficient for CD in pancreatic acinar cells. CD deficient mice showed normal 
      pancreatic development and autophagic activity, although LC3-II, which is a 
      marker of the autophagosome, accumulates in both physiological and pancreatitis 
      conditions. Moreover, CD deficiency leads to accumulation of matured cathepsin B 
      (CB) and cathepsin L (CL) which are members of the cysteine protease family. We 
      therefore conclude that CD in pancreatic acinar cells is implicated in CB and CL 
      degradation but not in autophagic activity.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Mehanna, Sally
AU  - Mehanna S
AD  - Institute of Resource Development and Analysis, Kumamoto University, 2-2-1 Honjo, 
      Chuo-ku, Kumamoto 860-0811, Japan.
FAU - Suzuki, Chigure
AU  - Suzuki C
AD  - Department of Cell Biology and Neuroscience, Juntendo University Graduate School 
      of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
FAU - Shibata, Masahiro
AU  - Shibata M
AD  - Department of Morphological Sciences, Kagoshima University Graduate School of 
      Medical and Dental Sciences, 8-35-1, Sakuragaoka, Kagoshima 890-8544, Japan.
FAU - Sunabori, Takehiko
AU  - Sunabori T
AD  - Department of Cell Biology and Neuroscience, Juntendo University Graduate School 
      of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
FAU - Imanaka, Takanobu
AU  - Imanaka T
AD  - Institute of Resource Development and Analysis, Kumamoto University, 2-2-1 Honjo, 
      Chuo-ku, Kumamoto 860-0811, Japan.
FAU - Araki, Kimi
AU  - Araki K
AD  - Institute of Resource Development and Analysis, Kumamoto University, 2-2-1 Honjo, 
      Chuo-ku, Kumamoto 860-0811, Japan.
FAU - Yamamura, Ken-ichi
AU  - Yamamura K
AD  - Institute of Resource Development and Analysis, Kumamoto University, 2-2-1 Honjo, 
      Chuo-ku, Kumamoto 860-0811, Japan.
FAU - Uchiyama, Yasuo
AU  - Uchiyama Y
AD  - Department of Cell Biology and Neuroscience, Juntendo University Graduate School 
      of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
FAU - Ohmuraya, Masaki
AU  - Ohmuraya M
AD  - Institute of Resource Development and Analysis, Kumamoto University, 2-2-1 Honjo, 
      Chuo-ku, Kumamoto 860-0811, Japan. Electronic address: ohmuraya@kumamoto-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151209
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - EC 3.4.22.1 (Cathepsin B)
RN  - EC 3.4.22.1 (Ctsb protein, mouse)
RN  - EC 3.4.22.15 (Cathepsin L)
RN  - EC 3.4.22.15 (Ctsl protein, mouse)
RN  - EC 3.4.23.5 (Cathepsin D)
RN  - EC 3.4.23.5 (Ctsd protein, mouse)
SB  - IM
MH  - Acinar Cells/*metabolism/*pathology
MH  - Animals
MH  - Autophagy
MH  - Cathepsin B/*metabolism
MH  - Cathepsin D/*metabolism
MH  - Cathepsin L/*metabolism
MH  - Cells, Cultured
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Pancreatitis/*metabolism/pathology
OTO - NOTNLM
OT  - Acute pancreatitis
OT  - Autophagy
OT  - Cathepsin D
OT  - Cysteine proteases
EDAT- 2015/12/20 06:00
MHDA- 2016/05/28 06:00
CRDT- 2015/12/20 06:00
PHST- 2015/11/19 00:00 [received]
PHST- 2015/12/01 00:00 [accepted]
PHST- 2015/12/20 06:00 [entrez]
PHST- 2015/12/20 06:00 [pubmed]
PHST- 2016/05/28 06:00 [medline]
AID - S0006-291X(15)31007-X [pii]
AID - 10.1016/j.bbrc.2015.12.002 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2016 Jan 15;469(3):405-11. doi: 
      10.1016/j.bbrc.2015.12.002. Epub 2015 Dec 9.