PMID- 26683206
OWN - NLM
STAT- MEDLINE
DCOM- 20160909
LR  - 20181113
IS  - 1472-6882 (Electronic)
IS  - 1472-6882 (Linking)
VI  - 15
DP  - 2015 Dec 18
TI  - The antioxidative effects of acidic-, alkalic-, and enzymatic-extractable 
      mycelium zinc polysaccharides by Pleurotus djamor on liver and kidney of 
      streptozocin-induced diabetic mice.
PG  - 440
LID - 10.1186/s12906-015-0964-1 [doi]
LID - 440
AB  - BACKGROUND: Edible mushrooms, especially the genus of Pleurotus, have been well 
      studied for their nutrition as well as non-toxic medicinal properties. Recently, 
      much attention has been paid to the therapeutic values of mushrooms in genus of 
      Pleurotus with diabetes mellitus (DM), which was a complex metabolic disorder 
      that induced by increased oxidative stress and characterized by hyperglycemia. 
      However, scare attention has been paid to polysaccharides from P. djamor. 
      Meanwhile, zinc is an essential trace element in the human body and it 
      participates in various pathways of metabolism. Therefore, the objective of 
      present study was aimed to evaluate the protective effects of the three 
      extractable mycelium zinc polysaccharides (MZPS), including acidic-MZPS 
      (Ac-MZPS), alkalic-MZPS (Al-MZPS) and enzymatic-MZPS (En-MZPS), on the liver and 
      kidneys in diabetic mice induced by streptozocin (STZ) aiming to better 
      understand the possible hypoglycemic mechanisms and their health benefits. 
      METHODS: The Ac-, Al-, and En-MZPS were extracted with hydrochloric acid (1 M), 
      sodium hydroxide (1 M) and snailase (4 %) from P. djamor zinc-enriched mycelium, 
      respectively. The diabetic mice were induced by injection of STZ. Besides the 
      histopathological analyses of liver and kidney, the following biochemical 
      analysis were processed to investigate the antioxidative effects, including 
      activities of superoxide dismutase (SOD), GSH peroxide (GSH-Px) and catalase 
      (CAT), and contents of malondialdehyde (MDA) in liver and kidney homogenate; 
      activities of alamine aminotransferase (ALT) and aspertate aminotransferase 
      (AST), and levels of urea nitrogen (BUN), creatinine (CRE), total cholesterol 
      (TC), albumin (ALB), high-density lipoprotein cholesterol (HDL-C), low-density 
      lipoprotein cholesterol (LDL-C) and very low-density lipoprotein cholesterol 
      (VLDL-C) in serum. RESULTS: Results showed that the activities of SOD, GSH-Px and 
      CAT were significantly increased, the MDA contents remarkably reduced, and the 
      values of ALT, AST, BUN, CRE, TC, LDL-C and HDL-C observably mitigated in the 
      liver, kidneys and serum of diabetic mice by these three polysaccharides 
      treatment. Biochemical and histopathological analyses also showed that MZPS could 
      alleviate liver and kidneys injury. CONCLUSION: These results demonstrated that 
      Ac-, Al-, and En-MZPS possessed potent antioxidant activities, and could be used 
      as a potentially functional food for the prevention of diabetes and its 
      complications induced by STZ.
FAU - Zhang, Jianjun
AU  - Zhang J
AD  - College of Life Science, Shandong Agricultural University, Taian, 271018, PR 
      China.
FAU - Meng, Guangyuan
AU  - Meng G
AD  - The Central Hospital of Taian, Taian, Shandong, 271000, PR China.
FAU - Zhang, Chen
AU  - Zhang C
AD  - College of Life Science, Shandong Agricultural University, Taian, 271018, PR 
      China.
FAU - Lin, Lin
AU  - Lin L
AD  - College of Life Science, Shandong Agricultural University, Taian, 271018, PR 
      China.
FAU - Xu, Nuo
AU  - Xu N
AD  - College of Life Science, Shandong Agricultural University, Taian, 271018, PR 
      China.
FAU - Liu, Min
AU  - Liu M
AD  - College of Life Science, Shandong Agricultural University, Taian, 271018, PR 
      China.
FAU - Cui, Fangyuan
AU  - Cui F
AD  - College of Life Science, Shandong Agricultural University, Taian, 271018, PR 
      China.
FAU - Jia, Le
AU  - Jia L
AD  - College of Life Science, Shandong Agricultural University, Taian, 271018, PR 
      China. jiale0525@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151218
PL  - England
TA  - BMC Complement Altern Med
JT  - BMC complementary and alternative medicine
JID - 101088661
RN  - 0 (Antioxidants)
RN  - 0 (Polysaccharides)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 5W494URQ81 (Streptozocin)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - GAN16C9B8O (Glutathione)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Animals
MH  - Antioxidants/*administration & dosage/chemistry/isolation & purification
MH  - Catalase/metabolism
MH  - Diabetes Mellitus, Experimental/*drug therapy
MH  - Female
MH  - Glutathione/metabolism
MH  - Humans
MH  - Kidney/drug effects/enzymology/metabolism
MH  - Liver/drug effects/enzymology/metabolism
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Mice
MH  - Mycelium/chemistry
MH  - Oxidative Stress/drug effects
MH  - Pleurotus/*chemistry
MH  - Polysaccharides/*administration & dosage/chemistry/isolation & purification
MH  - Streptozocin
MH  - Superoxide Dismutase/metabolism
MH  - Zinc/chemistry
PMC - PMC4683725
EDAT- 2015/12/20 06:00
MHDA- 2016/09/10 06:00
PMCR- 2015/12/18
CRDT- 2015/12/20 06:00
PHST- 2015/07/17 00:00 [received]
PHST- 2015/12/09 00:00 [accepted]
PHST- 2015/12/20 06:00 [entrez]
PHST- 2015/12/20 06:00 [pubmed]
PHST- 2016/09/10 06:00 [medline]
PHST- 2015/12/18 00:00 [pmc-release]
AID - 10.1186/s12906-015-0964-1 [pii]
AID - 964 [pii]
AID - 10.1186/s12906-015-0964-1 [doi]
PST - epublish
SO  - BMC Complement Altern Med. 2015 Dec 18;15:440. doi: 10.1186/s12906-015-0964-1.