PMID- 26686560
OWN - NLM
STAT- MEDLINE
DCOM- 20160725
LR  - 20181202
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1426
DP  - 2015 Dec 24
TI  - Determination of organophosphate diesters in urine samples by a high-sensitivity 
      method based on ultra high pressure liquid chromatography-triple quadrupole-mass 
      spectrometry.
PG  - 154-60
LID - S0021-9673(15)01626-X [pii]
LID - 10.1016/j.chroma.2015.11.018 [doi]
AB  - Organophosphate (OP) diesters in urine samples have potential use as biomarkers 
      of organism exposure to environmentally relevant OP triester precursors and in 
      particular OP triester flame retardants. This present study developed a 
      quantitatively sensitive ultra high pressure liquid chromatography (UHPLC-MS) 
      based method for urine and the determination of OP diesters (i.e. diphenyl 
      phosphate (DPHP), bis(2-chloroethyl) phosphate (BCEP), bis(2-chloroisopropyl) 
      phosphate (BDCIPP), di-n-butyl phosphate (DNBP), di(2-ethylhexyl) phosphate 
      (DEHP), bis(1-chloro-2-propyl) phosphate (BCIPP), and bis(2-butoxyethyl) 
      phosphate (BBOEP)). Fortified with the 7 OP diesters, 1mL of human urine sample 
      was cleaned up using weak anion exchange solid phase extraction and eluted with 
      high ionic strength ammonium acetate buffer. Subsequently, 4 non-chlorinated OP 
      diesters were directly determined using UHPLC-electrospray(-)-triple 
      quadrupole-MS (UHPLC-ESI(-)-QqQ-MS), and UHPLC-ESI(+)-QqQ-MS was used for 
      determination of 3 chlorinated OP diesters after methylation using diazomethane. 
      Recovery efficiencies of OP diesters ranged from 88 to 160% at three spiking 
      levels (0.4, 2 and 10ng/mL urine). Matrix effects (MEs) and method limits of 
      quantification (MLOQs) were 15-134% and 0.10-0.32ng/mL urine, respectively. 
      Concentrations of OP diesters in n=12 urine samples (from 4 Canadian residents, 
      2014) varied as follows, nd-<0.28 (DNBP), nd-1.29 (DPHP), nd-<0.28 (DEHP), 
      <0.16-12.33 (BCEP), nd-1.17 (BCDIPP) and nd-0.68ng/mL (BCIPP).
CI  - Copyright (c) 2015. Published by Elsevier B.V.
FAU - Su, Guanyong
AU  - Su G
AD  - Department of Chemistry, Carleton University, Ottawa, ON, Canada K1S 5B6. 
      Electronic address: guanyong.su85@gmail.com.
FAU - Letcher, Robert J
AU  - Letcher RJ
AD  - Department of Chemistry, Carleton University, Ottawa, ON, Canada K1S 5B6. 
      Electronic address: robert.letcher@canada.ca.
FAU - Yu, Hongxia
AU  - Yu H
AD  - State Key Laboratory of Pollution Control and Resource Reuse & School of the 
      Environment, Nanjing University, Nanjing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151110
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Buffers)
RN  - 0 (Esters)
RN  - 0 (Flame Retardants)
RN  - 0 (Organophosphates)
SB  - IM
MH  - Buffers
MH  - Chromatography, High Pressure Liquid/methods
MH  - Esters
MH  - Flame Retardants/analysis
MH  - Humans
MH  - Organophosphates/*urine
MH  - Osmolar Concentration
MH  - Solid Phase Extraction
MH  - Spectrometry, Mass, Electrospray Ionization
OTO - NOTNLM
OT  - Canadian residents
OT  - Organophosphate diesters
OT  - Organophosphate flame retardants
OT  - UHPLC-ESI-QqQ-MS
OT  - Urine
EDAT- 2015/12/22 06:00
MHDA- 2016/07/28 06:00
CRDT- 2015/12/22 06:00
PHST- 2015/07/02 00:00 [received]
PHST- 2015/10/14 00:00 [revised]
PHST- 2015/11/05 00:00 [accepted]
PHST- 2015/12/22 06:00 [entrez]
PHST- 2015/12/22 06:00 [pubmed]
PHST- 2016/07/28 06:00 [medline]
AID - S0021-9673(15)01626-X [pii]
AID - 10.1016/j.chroma.2015.11.018 [doi]
PST - ppublish
SO  - J Chromatogr A. 2015 Dec 24;1426:154-60. doi: 10.1016/j.chroma.2015.11.018. Epub 
      2015 Nov 10.