PMID- 26687302
OWN - NLM
STAT- MEDLINE
DCOM- 20161107
LR  - 20211203
IS  - 1573-6776 (Electronic)
IS  - 0141-5492 (Linking)
VI  - 38
IP  - 3
DP  - 2016 Mar
TI  - MicroRNA-144-3p inhibits proliferation and induces apoptosis of human salivary 
      adenoid carcinoma cells via targeting of mTOR.
PG  - 409-16
LID - 10.1007/s10529-015-2007-x [doi]
AB  - OBJECTIVES: To investigate the biological functions of microRNA-144-3p with 
      respect to proliferation and apoptosis of human salivary adenoid carcinoma cell 
      lines via mTOR. RESULTS: After transfection of microRNA-144-3p agomir, cell 
      viability assays confirmed that the salivary adenoid carcinoma cell (SACC) 
      proliferation was inhibited and apoptosis was induced. Dual luciferase reporter 
      assay validated that the mammalian target of rapamycin (mTOR) was a direct target 
      of miR-144-3p. Western blot, immunofluorescent analysis and a xenograft mouse 
      model of adenoid cystic carcinoma indicated that miR-144-3p was a tumor 
      suppressor and repressed mTOR expression and signaling in SACCs. CONCLUSIONS: 
      MicroRNA-144-3p inhibits proliferation and induces apoptosis of human salivary 
      adenoid carcinoma cells by downregulating mTOR expression in vitro and in vivo.
FAU - Huo, Fangyi
AU  - Huo F
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, 
      Department of Orthodontics, School & Hospital of Stomatology, Wuhan University, 
      237 Luoyu Road, Wuhan, Hubei Province, 430079, China. huofangyi1989070@163.com.
FAU - Zhang, Chen
AU  - Zhang C
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, 
      Department of Orthodontics, School & Hospital of Stomatology, Wuhan University, 
      237 Luoyu Road, Wuhan, Hubei Province, 430079, China. milesz@whu.edu.cn.
FAU - He, Hong
AU  - He H
AUID- ORCID: 0000-0001-5475-1641
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, 
      Department of Orthodontics, School & Hospital of Stomatology, Wuhan University, 
      237 Luoyu Road, Wuhan, Hubei Province, 430079, China. 1755850704@qq.com.
FAU - Wang, Yunlong
AU  - Wang Y
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, 
      Department of Orthodontics, School & Hospital of Stomatology, Wuhan University, 
      237 Luoyu Road, Wuhan, Hubei Province, 430079, China. wang200631550010@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151219
PL  - Netherlands
TA  - Biotechnol Lett
JT  - Biotechnology letters
JID - 8008051
RN  - 0 (MIRN144 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - *Apoptosis
MH  - Cell Line, Tumor
MH  - *Cell Proliferation
MH  - Cell Survival
MH  - Epithelial Cells/*physiology
MH  - Gene Expression Profiling
MH  - Humans
MH  - MicroRNAs/*metabolism
MH  - TOR Serine-Threonine Kinases/*antagonists & inhibitors
OTO - NOTNLM
OT  - Adenoid cystic carcinoma
OT  - Apoptosis
OT  - MTOR
OT  - MicroRNA-144-3p
OT  - Proliferation
EDAT- 2015/12/22 06:00
MHDA- 2016/11/08 06:00
CRDT- 2015/12/22 06:00
PHST- 2015/10/09 00:00 [received]
PHST- 2015/11/17 00:00 [accepted]
PHST- 2015/12/22 06:00 [entrez]
PHST- 2015/12/22 06:00 [pubmed]
PHST- 2016/11/08 06:00 [medline]
AID - 10.1007/s10529-015-2007-x [pii]
AID - 10.1007/s10529-015-2007-x [doi]
PST - ppublish
SO  - Biotechnol Lett. 2016 Mar;38(3):409-16. doi: 10.1007/s10529-015-2007-x. Epub 2015 
      Dec 19.