PMID- 26688947
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20200325
IS  - 1000-1182 (Print)
IS  - 2618-0456 (Electronic)
IS  - 1000-1182 (Linking)
VI  - 33
IP  - 5
DP  - 2015 Oct
TI  - [Circadian variation of clock gene Per2 and cancer-related clock-controlled genes 
      in buccal mucosa carcinoma of golden hamster at different cancer stages].
PG  - 513-8
AB  - OBJECTIVE: This study investigates the circadian variation rules of the clock 
      gene Per2 and clock-controlled genes of vascular endothelial growth factor 
      (VEGF), Ki67, c-Myc, and P53 in different stages of carcinogenesis in buccal 
      mucosa carcinoma and their roles in the development of buccal mucosa carcinoma. 
      METHODS: Ninety Syrian golden hamsters were housed under. 12 h light/12 h dark 
      cycles. Dimethylbenzanthracene (DMBA) was used to establish the carcinoma model 
      by smearing the golden hamster buccal mucosa. Before DMBA painting and after 6 
      and 14 weeks, the hamsters were sacrificed at six time points within a period of 
      24 h (i.e., 4, 8, 12, 16, 20, and 24 h after light onset), and the normal buccal 
      mucosa, precancerous lesions, and cancer tissues were simultaneously obtained. 
      Hematoxylin and eosin stained sections were prepared to observe the canceration 
      of each tissue. Real time polymerase chain reaction was used to detect the mRNA 
      expression of Per2, VEGF, Ki67, c-Myc, and P53. Cosine analysis was employed to 
      determine the circadian-rhythm variations of Per2, VEGF, Ki67, c-Myc, and P53 
      mRNA expression in terms of median, amplitude, and acrophase. RESULTS: The 
      expression of Per2, VEGF, P53, and c-Myc mRNA in three different stages appeared 
      with circadian rhythms (P<0.05), whereas the Ki67 mRNA was expressed with 
      circadian rhythm only in normal and precancerous lesion stages (P<0.05). The 
      midline-estimating statistic of rhythms (MESORs) of Per2 and P53 mRNA were 
      significantly down-regulated with the development of cancer (P<0.05), whereas the 
      MESORs of VEGF, c-Myc, and Ki67 mRNA were up-regulated (P<0.05). The amplitude of 
      P53 mRNA significantly decreased with the development of cancer (P<0.05). 
      Moreover, compared with the normal group, the amplitudes of Per2, VEGF, Ki67, and 
      c-Myc mRNA significantly increased in precancerous lesions and cancer tissue 
      (P<0.05). In precancerous stage, the acrophases of Per2, VEGF, and c-Myc mRNA 
      were earlier than that in the normal group, whereas that of Ki67 and P53 mRNA 
      were delayed. CONCLUSION: The circadian-rhythm characteristics of the clock gene 
      Per2 and clock-controlled gene expression of VEGF, Ki67, c-Myc, and P53 mRNA have 
      changed with the occurrence and development of carcinoma.
FAU - Ye, Hua
AU  - Ye H
FAU - Yang, Kai
AU  - Yang K
FAU - Tan, Xuemei
AU  - Tan X
FAU - Zhao, Dan
AU  - Zhao D
FAU - Lu, Xiaoqiang
AU  - Lu X
FAU - Wang, Qingqing
AU  - Wang Q
LA  - chi
PT  - Journal Article
PL  - China
TA  - Hua Xi Kou Qiang Yi Xue Za Zhi
JT  - Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal 
      of stomatology
JID - 9422648
RN  - 0 (Period Circadian Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 57-97-6 (9,10-Dimethyl-1,2-benzanthracene)
SB  - IM
MH  - 9,10-Dimethyl-1,2-benzanthracene
MH  - Animals
MH  - Carcinogenesis
MH  - Carcinoma, Squamous Cell/metabolism
MH  - *Circadian Rhythm
MH  - Cricetinae
MH  - Mesocricetus
MH  - Mouth Mucosa/metabolism
MH  - Mouth Neoplasms/*metabolism
MH  - Neoplasm Staging
MH  - Period Circadian Proteins/*genetics/metabolism
MH  - RNA, Messenger
MH  - Real-Time Polymerase Chain Reaction
MH  - Vascular Endothelial Growth Factor A
PMC - PMC7030316
EDAT- 2015/12/23 06:00
MHDA- 2016/12/15 06:00
PMCR- 2015/10/01
CRDT- 2015/12/23 06:00
PHST- 2015/12/23 06:00 [entrez]
PHST- 2015/12/23 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
PHST- 2015/10/01 00:00 [pmc-release]
AID - wcjs-33-05-513 [pii]
AID - 1000-1182(2015)05-0513-06 [pii]
AID - 10.7518/hxkq.2015.05.016 [doi]
PST - ppublish
SO  - Hua Xi Kou Qiang Yi Xue Za Zhi. 2015 Oct;33(5):513-8. doi: 
      10.7518/hxkq.2015.05.016.