PMID- 26689327
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20181202
IS  - 1879-3177 (Electronic)
IS  - 0887-2333 (Print)
IS  - 0887-2333 (Linking)
VI  - 32
DP  - 2016 Apr
TI  - Thirdhand smoke: Chemical dynamics, cytotoxicity, and genotoxicity in outdoor and 
      indoor environments.
PG  - 220-31
LID - S0887-2333(15)30028-X [pii]
LID - 10.1016/j.tiv.2015.12.007 [doi]
AB  - We tested the toxicity of thirdhand smoke (THS) using two controlled laboratory 
      exposure scenarios and low levels of THS. One exposure modeled THS in a car 
      parked outdoors, while the second modeled THS in a room without sunlight. The 
      fabrics were exposed to cigarette smoke and then extracted in culture medium. 
      Concentrations of nicotine, nicotine related alkaloids, and tobacco specific 
      nitrosamines (TSNAs) were determined in fresh and aged extracts. The 
      concentration of TSNAs increased with aging in the indoor experiment. THS 
      extracts were used for cytotoxicity testing using mouse neural stem cells (mNSC), 
      human dermal fibroblasts (hDF) and human palatal mesenchyme cells (hPM). Extracts 
      from the car experiment inhibited mNSC proliferation in a live cell imaging assay 
      and induced single strand DNA breaks in mNSC and hDF. In the indoor experiment, 
      THS extracts made with medium containing serum proteins were significantly more 
      toxic than extracts made with basal medium, and mNSC and hPM were more sensitive 
      than hDF. These data indicate that: (1) aging of THS chemical differs on 
      different fabrics and differs with and without sunlight; (2) very few cigarettes 
      are sufficient to produce a toxic THS residue; and (3) protein enhances the 
      efficiency of extraction of cytotoxic chemicals.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Bahl, Vasundhra
AU  - Bahl V
AD  - Department of Cell Biology and Neuroscience, University of California, Riverside, 
      CA, USA; Environmental Toxicology Graduate Program, USA. Electronic address: 
      bahl.vasu@gmail.com.
FAU - Shim, Hyung Jun
AU  - Shim HJ
AD  - Department of Cell Biology and Neuroscience, University of California, Riverside, 
      CA, USA. Electronic address: shjun112@gmail.com.
FAU - Jacob, Peyton 3rd
AU  - Jacob P 3rd
AD  - Department of Clinical Pharmacology, University of California, San Francisco, CA, 
      USA. Electronic address: pjacob@medsfgh.ucsf.edu.
FAU - Dias, Kristen
AU  - Dias K
AD  - Department of Cell Biology and Neuroscience, University of California, Riverside, 
      CA, USA. Electronic address: kdias001@ucr.edu.
FAU - Schick, Suzaynn F
AU  - Schick SF
AD  - Department of Medicine, Division of Occupational and Environmental Medicine, 
      University of California, San Francisco, CA, USA. Electronic address: 
      suzaynn.schick@ucsf.edu.
FAU - Talbot, Prue
AU  - Talbot P
AD  - Department of Cell Biology and Neuroscience, University of California, Riverside, 
      CA, USA. Electronic address: talbot@ucr.edu.
LA  - eng
GR  - P30 DA012393/DA/NIDA NIH HHS/United States
GR  - S10 RR026437/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20151210
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
RN  - 0 (Air Pollutants)
RN  - 0 (Alkaloids)
RN  - 0 (Nitrosamines)
RN  - 0 (Tobacco Smoke Pollution)
SB  - IM
MH  - Air Pollutants/radiation effects/*toxicity
MH  - Air Pollution, Indoor/*adverse effects
MH  - Alkaloids/analysis
MH  - Animals
MH  - Automobiles
MH  - Cells, Cultured
MH  - DNA Damage
MH  - Female
MH  - Fibroblasts/drug effects
MH  - Humans
MH  - Mesenchymal Stem Cells/drug effects
MH  - Mice
MH  - Middle Aged
MH  - Neural Stem Cells/drug effects
MH  - Nitrosamines/analysis
MH  - Sunlight
MH  - *Textiles/analysis
MH  - Tobacco Smoke Pollution/*adverse effects
PMC - PMC5526588
MID - NIHMS878710
OTO - NOTNLM
OT  - Cigarette smoke
OT  - Nicotine
OT  - Stem cells
OT  - Thirdhand smoke
OT  - Tobacco
OT  - Tobacco specific nitrosamines
OT  - Toxicity
COIS- Conflict of Interest None.
EDAT- 2015/12/23 06:00
MHDA- 2016/12/15 06:00
PMCR- 2017/07/25
CRDT- 2015/12/23 06:00
PHST- 2015/10/12 00:00 [received]
PHST- 2015/11/18 00:00 [revised]
PHST- 2015/12/09 00:00 [accepted]
PHST- 2015/12/23 06:00 [entrez]
PHST- 2015/12/23 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
PHST- 2017/07/25 00:00 [pmc-release]
AID - S0887-2333(15)30028-X [pii]
AID - 10.1016/j.tiv.2015.12.007 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2016 Apr;32:220-31. doi: 10.1016/j.tiv.2015.12.007. Epub 2015 
      Dec 10.