PMID- 26689644
OWN - NLM
STAT- MEDLINE
DCOM- 20161031
LR  - 20181113
IS  - 1879-0240 (Electronic)
IS  - 0965-1748 (Print)
IS  - 0965-1748 (Linking)
VI  - 70
DP  - 2016 Mar
TI  - Protein kinase C modulates transcriptional activation by the juvenile hormone 
      receptor methoprene-tolerant.
PG  - 44-52
LID - S0965-1748(15)30075-8 [pii]
LID - 10.1016/j.ibmb.2015.12.001 [doi]
AB  - Juvenile hormone (JH) controls many biological events in insects by triggering 
      dramatic changes in gene expression in target cells. The Methoprene-tolerant 
      (MET) protein, an intracellular JH receptor, acts as a transcriptional regulator 
      and binds to the promoters of tissue- and stage-specific JH target genes when JH 
      is present. Our recent study has demonstrated that the transcriptional activation 
      by MET is modulated by a membrane-initiated JH signaling pathway, involving 
      phospholipase C (PLC) and calcium/calmodulin-dependent protein kinase II 
      (CaMKII). Here we report that protein kinase C (PKC) is another essential 
      intermediate of this pathway. PKC was activated by JH and this action was 
      PLC-dependent. Inhibition of the PKC activity substantially weakened the 
      JH-induced gene expression in mosquito cells. RNAi experiments indicated that 
      several PKC isoforms were involved in the JH action during the post-emergence 
      development of adult female mosquitoes. JH treatment considerably increased the 
      binding of MET to the promoters of JH response genes in cultured mosquito 
      abdomens that were collected from newly emerged female adults. The JH-induced DNA 
      binding of MET was hindered when the abdomens were treated with a PKC inhibitor 
      and JH. Therefore, the results suggest that PKC modulates the transactivation 
      activity of MET by enhancing the binding of MET to JH response elements in the JH 
      target genes. This mechanism may allow for variable and stage- and 
      tissue-specific genomic responses to JH.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Ojani, Reyhaneh
AU  - Ojani R
AD  - Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061, USA.
FAU - Liu, Pengcheng
AU  - Liu P
AD  - Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061, USA.
FAU - Fu, Xiaonan
AU  - Fu X
AD  - Program of Genetics, Bioinformatics, and Computational Biology, Virginia Tech, 
      Blacksburg, VA 24061, USA.
FAU - Zhu, Jinsong
AU  - Zhu J
AD  - Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061, USA. Electronic 
      address: zhujin@vt.edu.
LA  - eng
GR  - R01 AI099250/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20151213
PL  - England
TA  - Insect Biochem Mol Biol
JT  - Insect biochemistry and molecular biology
JID - 9207282
RN  - 0 (Juvenile Hormones)
RN  - 0 (Receptors, Cell Surface)
RN  - 8B830OJ2UX (Methoprene)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Aedes
MH  - Animals
MH  - Cell Line
MH  - Juvenile Hormones/metabolism/*pharmacology
MH  - Methoprene/*pharmacology
MH  - Protein Kinase C/*metabolism
MH  - Receptors, Cell Surface/*metabolism
MH  - Transcriptional Activation/*drug effects
PMC - PMC4767628
MID - NIHMS748196
OTO - NOTNLM
OT  - Gene regulation
OT  - Hormone receptor
OT  - Juvenile hormone
OT  - Phospholipase C
OT  - Protein kinase C
EDAT- 2015/12/23 06:00
MHDA- 2016/11/01 06:00
PMCR- 2017/03/01
CRDT- 2015/12/23 06:00
PHST- 2015/07/23 00:00 [received]
PHST- 2015/11/26 00:00 [revised]
PHST- 2015/12/09 00:00 [accepted]
PHST- 2017/03/01 00:00 [pmc-release]
PHST- 2015/12/23 06:00 [entrez]
PHST- 2015/12/23 06:00 [pubmed]
PHST- 2016/11/01 06:00 [medline]
AID - S0965-1748(15)30075-8 [pii]
AID - 10.1016/j.ibmb.2015.12.001 [doi]
PST - ppublish
SO  - Insect Biochem Mol Biol. 2016 Mar;70:44-52. doi: 10.1016/j.ibmb.2015.12.001. Epub 
      2015 Dec 13.