PMID- 26690105
OWN - NLM
STAT- MEDLINE
DCOM- 20160928
LR  - 20220408
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 20
IP  - 12
DP  - 2015 Dec 4
TI  - Fast Simultaneous Determination of 13 Nucleosides and Nucleobases in Cordyceps 
      sinensis by UHPLC-ESI-MS/MS.
PG  - 21816-25
LID - 10.3390/molecules201219807 [doi]
AB  - A reliable ultra-high-performance liquid chromatography-electrospray 
      ionization-tandem mass spectrometry (UHPLC-ESI-MS/MS) method for the fast 
      simultaneous determination of 13 nucleosides and nucleobases in Cordyceps 
      sinensis (C. sinensis) with 2-chloroadenosine as internal standard was developed 
      and validated. Samples were ultrasonically extracted in an ice bath thrice, and 
      the optimum analyte separation was performed on an ACQUITY UPLC(TM) HSS C18 
      column (100 mm x 2.1 mm, 1.8 mum) with gradient elution. All targeted analytes 
      were separated in 5.5 min. Furthermore, all calibration curves showed good linear 
      regression (r > 0.9970) within the test ranges, and the limits of quantitation 
      and detection of the 13 analytes were less than 150 and 75 ng/mL, respectively. 
      The relative standard deviations (RSDs) of intra- and inter-day precisions were 
      <6.23%. Recoveries of the quantified analytes ranged within 85.3%-117.3%, with 
      RSD < 6.18%. The developed UHPLC-ESI-MS/MS method was successfully applied to 
      determine nucleosides and nucleobases in 11 batches of C. sinensis samples from 
      different regions in China. The range for the total content in the analyzed 
      samples was 1329-2057 microg/g.
FAU - Zong, Shi-Yu
AU  - Zong SY
AD  - School of Pharmacy, Shanghai University of Traditional Chinese Medicine, No. 1200 
      Cailun Road, Pudong New District, Shanghai 201203, China. pashanhan@163.com.
AD  - Experimental Center of Teaching and Learning, Shanghai University of Traditional 
      Chinese Medicine, No. 1200 Cailun Road, Pudong New District, Shanghai 201203, 
      China. pashanhan@163.com.
FAU - Han, Han
AU  - Han H
AD  - Experimental Center of Teaching and Learning, Shanghai University of Traditional 
      Chinese Medicine, No. 1200 Cailun Road, Pudong New District, Shanghai 201203, 
      China. pashanhan@163.com.
FAU - Wang, Bing
AU  - Wang B
AD  - School of Pharmacy, Shanghai University of Traditional Chinese Medicine, No. 1200 
      Cailun Road, Pudong New District, Shanghai 201203, China. annabel_cn@163.com.
AD  - Experimental Center of Teaching and Learning, Shanghai University of Traditional 
      Chinese Medicine, No. 1200 Cailun Road, Pudong New District, Shanghai 201203, 
      China. annabel_cn@163.com.
FAU - Li, Ning
AU  - Li N
AD  - Division of Life Science and Center for Chinese Medicine, The Hong Kong 
      University of Science and Technology, Clear Water Bay Road, Hong Kong, China. 
      mailtolining@gmail.com.
FAU - Dong, Tina Ting-Xia
AU  - Dong TT
AD  - Division of Life Science and Center for Chinese Medicine, The Hong Kong 
      University of Science and Technology, Clear Water Bay Road, Hong Kong, China. 
      botina@ust.hk.
FAU - Zhang, Tong
AU  - Zhang T
AD  - School of Pharmacy, Shanghai University of Traditional Chinese Medicine, No. 1200 
      Cailun Road, Pudong New District, Shanghai 201203, China. zhangtdmj@hotmail.com.
AD  - Experimental Center of Teaching and Learning, Shanghai University of Traditional 
      Chinese Medicine, No. 1200 Cailun Road, Pudong New District, Shanghai 201203, 
      China. zhangtdmj@hotmail.com.
FAU - Tsim, Karl W K
AU  - Tsim KW
AD  - Division of Life Science and Center for Chinese Medicine, The Hong Kong 
      University of Science and Technology, Clear Water Bay Road, Hong Kong, China. 
      botsim@ust.hk.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151204
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Nucleosides)
RN  - 0 (Pyrimidines)
SB  - IM
MH  - Calibration
MH  - Chromatography, High Pressure Liquid
MH  - Cordyceps/chemistry
MH  - Limit of Detection
MH  - Nucleosides/chemistry/*isolation & purification
MH  - Pyrimidines/chemistry/isolation & purification
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Tandem Mass Spectrometry
PMC - PMC6332315
OTO - NOTNLM
OT  - Cordyceps sinensis
OT  - UHPLC-ESI-MS/MS
OT  - nucleobases
OT  - nucleosides
COIS- The authors declare there is no conflict of interest.
EDAT- 2015/12/23 06:00
MHDA- 2016/09/30 06:00
PMCR- 2015/12/04
CRDT- 2015/12/23 06:00
PHST- 2015/11/04 00:00 [received]
PHST- 2015/11/29 00:00 [revised]
PHST- 2015/12/01 00:00 [accepted]
PHST- 2015/12/23 06:00 [entrez]
PHST- 2015/12/23 06:00 [pubmed]
PHST- 2016/09/30 06:00 [medline]
PHST- 2015/12/04 00:00 [pmc-release]
AID - molecules201219807 [pii]
AID - molecules-20-19807 [pii]
AID - 10.3390/molecules201219807 [doi]
PST - epublish
SO  - Molecules. 2015 Dec 4;20(12):21816-25. doi: 10.3390/molecules201219807.