PMID- 26690404 OWN - NLM STAT- MEDLINE DCOM- 20161005 LR - 20200225 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 20 IP - 12 DP - 2015 Dec 11 TI - Multi-Optimization of Ultrasonic-Assisted Enzymatic Extraction of Atratylodes macrocephala Polysaccharides and Antioxidants Using Response Surface Methodology and Desirability Function Approach. PG - 22220-35 LID - 10.3390/molecules201219837 [doi] AB - Rhizoma Atractylodes macrocephala polysaccharides (RAMP) have been reported to have a variety of important biological activities. In this study, an ultrasonic-assisted enzymatic extraction (UAEE) was employed to obtain the highest extraction yield and strongest antioxidant activity of RAMP and optimized by a multi-response optimization process. A three-level four-factor Box-Behnken design (BBD) was performed as response surface methodology (RSM) with desirability function (DF) to attain the optimal extraction parameters. The DPPH scavenging percentage was used to represent the antioxidant ability of RAMP. The maximum D value (0.328), along with the maximum yield (59.92%) and DPPH scavenging percentage (13.28%) were achieved at 90.54 min, 57.99 degrees C, 1.95% cellulase and 225.29 W. These values were further validated and found to be in good agreement with the predicted values. Compared to the other extraction methods, both the yield and scavenging percentage of RAMP obtained by UAEE was favorable and the method appeared to be time-saving and of high efficiency. These results demostrated that UAEE is an appropriate and effective extraction technique. Moreover, RSM with DF approach has been proved to be adequate for the design and optimization of the extraction parameters for RAMP. This work has a wide range of implications for the design and operation of polysaccharide extraction processes. FAU - Pu, Jin-Bao AU - Pu JB AD - Development and Research Center of Official Silkworm Resources, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, China. pjb0225@163.com. AD - Key Laboratory of Research and Development of Chinese Medicine of Zhejiang Province, Hangzhou 310007, China. pjb0225@163.com. FAU - Xia, Bo-Hou AU - Xia BH AD - College of Pharmacy, Hunan Chinese Medical University, Changsha 410208, China. xiabohou@163.com. FAU - Hu, Yi-Juan AU - Hu YJ AD - Development and Research Center of Official Silkworm Resources, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, China. huyijuan604@163.com. AD - Key Laboratory of Research and Development of Chinese Medicine of Zhejiang Province, Hangzhou 310007, China. huyijuan604@163.com. FAU - Zhang, Hong-Jian AU - Zhang HJ AD - Development and Research Center of Official Silkworm Resources, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, China. jian871211@sina.com. AD - Key Laboratory of Research and Development of Chinese Medicine of Zhejiang Province, Hangzhou 310007, China. jian871211@sina.com. FAU - Chen, Jing AU - Chen J AD - College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310015, China. cj00123@zcmu.edu.cn. FAU - Zhou, Jie AU - Zhou J AD - Development and Research Center of Official Silkworm Resources, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, China. Kenzei@163.com. AD - Key Laboratory of Research and Development of Chinese Medicine of Zhejiang Province, Hangzhou 310007, China. Kenzei@163.com. FAU - Liang, Wei-Qing AU - Liang WQ AD - Development and Research Center of Official Silkworm Resources, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, China. jxlwq22@163.com. AD - Key Laboratory of Research and Development of Chinese Medicine of Zhejiang Province, Hangzhou 310007, China. jxlwq22@163.com. FAU - Xu, Pan AU - Xu P AD - Development and Research Center of Official Silkworm Resources, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, China. xpan840520@163.com. AD - Key Laboratory of Research and Development of Chinese Medicine of Zhejiang Province, Hangzhou 310007, China. xpan840520@163.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20151211 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Antioxidants) RN - 0 (Biphenyl Compounds) RN - 0 (Picrates) RN - 0 (Plant Extracts) RN - 0 (Polysaccharides) RN - DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl) RN - EC 3.2.1.4 (Cellulase) SB - IM MH - Antioxidants/*chemistry/isolation & purification MH - Asteraceae/*chemistry MH - Biphenyl Compounds/antagonists & inhibitors MH - Cellulase/chemistry MH - Factor Analysis, Statistical MH - Liquid-Liquid Extraction/*methods MH - Picrates/antagonists & inhibitors MH - Plant Extracts/*chemistry MH - Polysaccharides/*chemistry/isolation & purification MH - Sonication PMC - PMC6332337 OTO - NOTNLM OT - Rhizoma Atractylodes macrocephala polysaccharides OT - antioxidant activity OT - desirability function approach OT - multi-optimization OT - response surface methodology OT - ultrasonic-assisted enzymatic extraction COIS- The authors declare no conflict of interest. EDAT- 2015/12/23 06:00 MHDA- 2016/10/07 06:00 PMCR- 2015/12/11 CRDT- 2015/12/23 06:00 PHST- 2015/10/14 00:00 [received] PHST- 2015/11/30 00:00 [revised] PHST- 2015/12/04 00:00 [accepted] PHST- 2015/12/23 06:00 [entrez] PHST- 2015/12/23 06:00 [pubmed] PHST- 2016/10/07 06:00 [medline] PHST- 2015/12/11 00:00 [pmc-release] AID - molecules201219837 [pii] AID - molecules-20-19837 [pii] AID - 10.3390/molecules201219837 [doi] PST - epublish SO - Molecules. 2015 Dec 11;20(12):22220-35. doi: 10.3390/molecules201219837.