PMID- 26690426
OWN - NLM
STAT- MEDLINE
DCOM- 20160926
LR  - 20181113
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 16
IP  - 12
DP  - 2015 Dec 11
TI  - Comparative Studies of 5S rDNA Profiles and Cyt b Sequences in two Onychostoma 
      Species (Cyprinidae).
PG  - 29663-72
LID - 10.3390/ijms161226193 [doi]
AB  - Onychostoma barbatulum and O. alticorpus, two primarily freshwater cyprinid fish, 
      have similar morphological characters and partially overlapping ecological 
      habitats. In order to explore the genetic differences between these two species, 
      chromosomal characteristics and genetic variations were examined by fluorescence 
      in situ hybridization (FISH) of 5S rDNA and cytochrome (Cyt) b gene analysis. Ten 
      specimens of O. barbatulum and O. alticorpus were collected from the Nanzihsian 
      Stream in southern Taiwan. FISH revealed that the 5S rDNA loci of O. barbatulum 
      and O. alticorpus were found at a pericentromeric and subtelomeric position, 
      respectively, in a pair of submetacentric chromosomes. Cyt b genes were amplified 
      and sequenced from five individuals of each species. Intraspecific genetic 
      distances ranged from 0.001-0.004 in O. barbatulum and from 0.001-0.006 in O. 
      alticorpus. Genetic distances between these two species ranged from 0.132-0.142. 
      The phylogenetic tree showed these two species are not sister species. In 
      conclusion, FISH cytogenetic information and Cyt b gene analyses indicated that 
      these two species have significantly different genetic characteristics; 
      nevertheless, their morphological similarities may be due to environmental 
      adaptation.
FAU - Han, Chiao-Chuan
AU  - Han CC
AD  - National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan. 
      hancc@nmmba.gov.tw.
AD  - Graduate Institute of Marine Biology, National Dong Hwa University, Hualien 974, 
      Taiwan. hancc@nmmba.gov.tw.
FAU - Yen, Tsair-Bor
AU  - Yen TB
AD  - Department of Tropical Agriculture and International Cooperation, National 
      Pingtung University of Science and Technology, Pingtung 912, Taiwan. 
      tbyen@mail.npust.edu.tw.
FAU - Chen, Nian-Cih
AU  - Chen NC
AD  - Institute of Aquaculture, National Pingtung University of Science and Technology, 
      Pingtung 912, Taiwan. niancih0701@gmail.com.
FAU - Tseng, Mei-Chen
AU  - Tseng MC
AD  - Institute of Aquaculture, National Pingtung University of Science and Technology, 
      Pingtung 912, Taiwan. mctseng@mail.npust.edu.tw.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151211
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Fish Proteins)
RN  - 9035-37-4 (Cytochromes b)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Conserved Sequence
MH  - Cyprinidae/*genetics
MH  - Cytochromes b/*genetics
MH  - DNA, Ribosomal/*genetics
MH  - Fish Proteins/*genetics
MH  - Molecular Sequence Data
MH  - Sequence Analysis, DNA
MH  - Species Specificity
PMC - PMC4691136
OTO - NOTNLM
OT  - Taiwan
OT  - adaptation
OT  - cyprinid
OT  - cytogenetic
OT  - genetic distance
EDAT- 2015/12/23 06:00
MHDA- 2016/09/27 06:00
PMCR- 2015/12/01
CRDT- 2015/12/23 06:00
PHST- 2015/09/24 00:00 [received]
PHST- 2015/11/27 00:00 [revised]
PHST- 2015/12/01 00:00 [accepted]
PHST- 2015/12/23 06:00 [entrez]
PHST- 2015/12/23 06:00 [pubmed]
PHST- 2016/09/27 06:00 [medline]
PHST- 2015/12/01 00:00 [pmc-release]
AID - ijms161226193 [pii]
AID - ijms-16-26193 [pii]
AID - 10.3390/ijms161226193 [doi]
PST - epublish
SO  - Int J Mol Sci. 2015 Dec 11;16(12):29663-72. doi: 10.3390/ijms161226193.