PMID- 26690485
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151222
LR  - 20201001
IS  - 2076-393X (Print)
IS  - 2076-393X (Electronic)
IS  - 2076-393X (Linking)
VI  - 3
IP  - 4
DP  - 2015 Dec 11
TI  - Dendritic Cell-Based Adjuvant Vaccination Targeting Wilms' Tumor 1 in Patients 
      with Advanced Colorectal Cancer.
PG  - 1004-18
LID - 10.3390/vaccines3041004 [doi]
AB  - Despite significant recent advances in the development of immune checkpoint 
      inhibitors, the treatment of advanced colorectal cancer involving metastasis to 
      distant organs remains challenging. We conducted a phase I study to investigate 
      the safety and immunogenicity of Wilms' tumor (WT1) class I/II peptides-pulsed 
      dendritic cell DC vaccination for patients with advanced colorectal cancer. 
      Standard treatment comprising surgical resection and chemotherapy was followed by 
      one course of seven biweekly administrations of 1-2 x 10(7) DCs with 1-2 KE of 
      OK-432 (streptococcal preparation) in three patients. Clinical efficacy was 
      confirmed based on WT1 expression using immunohistochemistry on paraffin-embedded 
      tissues and immune monitoring using tetramer analysis and enzyme-linked 
      immunosorbent spot (ELISPOT) assays. WT1 expression with human leukocyte antigen 
      (HLA)-class I molecules was detected in surgical resected tissues. Adverse 
      reactions to DC vaccinations were tolerable under an adjuvant setting. 
      WT1-specific cytotoxic T cells were detected by both modified 
      WT1-peptide/HLA-A*24:02 tetramer analysis and/or interferon-gamma-producing cells 
      through the use of ELISPOT assays after the first DC vaccination. Immunity 
      acquired from DC vaccination persisted for two years with prolonged disease-free 
      and overall survival. The present study indicated that DC vaccination targeting 
      WT1 demonstrated the safety and immunogenicity as an adjuvant therapy in patients 
      with resectable advanced colorectal cancer.
FAU - Shimodaira, Shigetaka
AU  - Shimodaira S
AD  - Center for Advanced Cell Therapy, Shinshu University Hospital, Matsumoto 8621, 
      Japan. shimodai@shinshu-u.ac.jp.
FAU - Sano, Kenji
AU  - Sano K
AD  - Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto 8621, 
      Japan. kenjisa@shinshu-u.ac.jp.
FAU - Hirabayashi, Koichi
AU  - Hirabayashi K
AD  - Center for Advanced Cell Therapy, Shinshu University Hospital, Matsumoto 8621, 
      Japan. kohira@shinshu-u.ac.jp.
FAU - Koya, Terutsugu
AU  - Koya T
AD  - Center for Advanced Cell Therapy, Shinshu University Hospital, Matsumoto 8621, 
      Japan. koya@shinshu-u.ac.jp.
FAU - Higuchi, Yumiko
AU  - Higuchi Y
AD  - Center for Advanced Cell Therapy, Shinshu University Hospital, Matsumoto 8621, 
      Japan. sasa0922@shinshu-u.ac.jp.
FAU - Mizuno, Yumiko
AU  - Mizuno Y
AD  - Center for Advanced Cell Therapy, Shinshu University Hospital, Matsumoto 8621, 
      Japan. purimeron@shinshu-u.ac.jp.
FAU - Yamaoka, Naoko
AU  - Yamaoka N
AD  - Center for Advanced Cell Therapy, Shinshu University Hospital, Matsumoto 8621, 
      Japan. naoko4152@shinshu-u.ac.jp.
FAU - Yuzawa, Miki
AU  - Yuzawa M
AD  - Center for Advanced Cell Therapy, Shinshu University Hospital, Matsumoto 8621, 
      Japan. m1k1yz@shinshu-u.ac.jp.
FAU - Kobayashi, Takashi
AU  - Kobayashi T
AD  - Shinshu Cancer Center, Shinshu University Hospital, Matsumoto 8621, Japan. 
      takob@shinshu-u.ac.jp.
FAU - Ito, Kenichi
AU  - Ito K
AD  - Department of Breast and Endocrine Surgery, Shinshu University Hospital, 
      Matsumoto 8621, Japan. kenito@shinshu-u.ac.jp.
FAU - Koizumi, Tomonobu
AU  - Koizumi T
AD  - Shinshu Cancer Center, Shinshu University Hospital, Matsumoto 8621, Japan. 
      tomonobu@shinshu-u.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20151211
PL  - Switzerland
TA  - Vaccines (Basel)
JT  - Vaccines
JID - 101629355
PMC - PMC4693229
OTO - NOTNLM
OT  - Wilms' tumor 1
OT  - colorectal cancer
OT  - dendritic cell vaccines
OT  - enzyme-linked immunosorbent spot assay
OT  - immunohistochemistry
OT  - tetramer analysis
EDAT- 2015/12/23 06:00
MHDA- 2015/12/23 06:01
PMCR- 2015/12/11
CRDT- 2015/12/23 06:00
PHST- 2015/09/30 00:00 [received]
PHST- 2015/11/19 00:00 [revised]
PHST- 2015/12/07 00:00 [accepted]
PHST- 2015/12/23 06:00 [entrez]
PHST- 2015/12/23 06:00 [pubmed]
PHST- 2015/12/23 06:01 [medline]
PHST- 2015/12/11 00:00 [pmc-release]
AID - vaccines3041004 [pii]
AID - vaccines-03-01004 [pii]
AID - 10.3390/vaccines3041004 [doi]
PST - epublish
SO  - Vaccines (Basel). 2015 Dec 11;3(4):1004-18. doi: 10.3390/vaccines3041004.