PMID- 26690724
OWN - NLM
STAT- MEDLINE
DCOM- 20170116
LR  - 20170116
IS  - 1740-0929 (Electronic)
IS  - 1344-3941 (Linking)
VI  - 87
IP  - 3
DP  - 2016 Mar
TI  - Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine 
      preimplantation development.
PG  - 457-61
LID - 10.1111/asj.12538 [doi]
AB  - Nuclear autoantigenic sperm protein (NASP) is associated with DNA replication, 
      cell proliferation, and cell cycle progression through its specific binding to 
      histones. The aim of this study was to examine the roles of NASP in bovine 
      preimplantation embryonic development. Using NASP gene knockdown (KD), we 
      confirmed the reduction of NASP messenger RNA (mRNA) expression during 
      preimplantation development. NASP KD did not affect cleavage but significantly 
      decreased development of embryos into the blastocyst stage. Furthermore, 
      blastocyst hatching was significantly decreased in NASP KD embryos. Cell numbers 
      in the inner cell mass of NASP KD blastocysts were also decreased compared to 
      those of controls. These results suggest that NASP mRNA expression is required 
      for preimplantation development into the blastocyst stage in cattle.
CI  - (c) 2015 Japanese Society of Animal Science.
FAU - Nagatomo, Hiroaki
AU  - Nagatomo H
AD  - Advanced Biotechnology Center, University of Yamanashi, Kofu, Japan.
FAU - Kohri, Nanami
AU  - Kohri N
AD  - Laboratory of Animal Breeding and Reproduction, Graduate School of Agriculture, 
      Hokkaido University, Sapporo, Japan.
FAU - Akizawa, Hiroki
AU  - Akizawa H
AD  - Laboratory of Animal Breeding and Reproduction, Graduate School of Agriculture, 
      Hokkaido University, Sapporo, Japan.
FAU - Hoshino, Yumi
AU  - Hoshino Y
AD  - Laboratory of Animal Reproduction, Graduate School of Agricultural Science, 
      Tohoku University, Sendai, Japan.
FAU - Yamauchi, Nobuhiko
AU  - Yamauchi N
AD  - Department of Animal and Marine Bioresource Sciences, Graduate School of 
      Agriculture, Kyushu University, Fukuoka, Japan.
FAU - Kono, Tomohiro
AU  - Kono T
AD  - Department of BioScience, Tokyo University of Agriculture, Tokyo, Japan.
FAU - Takahashi, Masashi
AU  - Takahashi M
AD  - Laboratory of Animal Breeding and Reproduction, Graduate School of Agriculture, 
      Hokkaido University, Sapporo, Japan.
FAU - Kawahara, Manabu
AU  - Kawahara M
AD  - Laboratory of Animal Breeding and Reproduction, Graduate School of Agriculture, 
      Hokkaido University, Sapporo, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151222
PL  - Australia
TA  - Anim Sci J
JT  - Animal science journal = Nihon chikusan Gakkaiho
JID - 100956805
RN  - 0 (Autoantigens)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Autoantigens/*genetics
MH  - Cattle
MH  - Embryonic Development/*genetics
MH  - Female
MH  - Gene Expression/*genetics
MH  - Male
MH  - Nuclear Proteins/*genetics
MH  - RNA, Messenger/*genetics
OTO - NOTNLM
OT  - NASP
OT  - bovine embryo
OT  - knockdown
EDAT- 2015/12/23 06:00
MHDA- 2017/01/17 06:00
CRDT- 2015/12/23 06:00
PHST- 2015/07/22 00:00 [received]
PHST- 2015/08/11 00:00 [revised]
PHST- 2015/08/20 00:00 [accepted]
PHST- 2015/12/23 06:00 [entrez]
PHST- 2015/12/23 06:00 [pubmed]
PHST- 2017/01/17 06:00 [medline]
AID - 10.1111/asj.12538 [doi]
PST - ppublish
SO  - Anim Sci J. 2016 Mar;87(3):457-61. doi: 10.1111/asj.12538. Epub 2015 Dec 22.