PMID- 26691653
OWN - NLM
STAT- MEDLINE
DCOM- 20170410
LR  - 20170410
IS  - 1099-1263 (Electronic)
IS  - 0260-437X (Linking)
VI  - 36
IP  - 9
DP  - 2016 Sep
TI  - Discrimination of skin sensitizers from non-sensitizers by interleukin-1alpha and 
      interleukin-6 production on cultured human keratinocytes.
PG  - 1129-36
LID - 10.1002/jat.3274 [doi]
AB  - In vitro testing methods for classifying sensitizers could be valuable 
      alternatives to in vivo sensitization testing using animal models, such as the 
      murine local lymph node assay (LLNA) and the guinea pig maximization test (GMT), 
      but there remains a need for in vitro methods that are more accurate and simpler 
      to distinguish skin sensitizers from non-sensitizers. Thus, the aim of our study 
      was to establish an in vitro assay as a screening tool for detecting skin 
      sensitizers using the human keratinocyte cell line, HaCaT. HaCaT cells were 
      exposed to 16 relevant skin sensitizers and 6 skin non-sensitizers. The highest 
      dose used was the dose causing 75% cell viability (CV75) that we determined by an 
      MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. The 
      levels of extracellular production of interleukin-1alpha (IL-1alpha) and IL-6 were 
      measured. The sensitivity of IL-1alpha was 63%, specificity was 83% and accuracy was 
      68%. In the case of IL-6, sensitivity: 69%, specificity: 83% and accuracy: 73%. 
      Thus, this study suggests that measuring extracellular production of 
      pro-inflammatory cytokines IL-1alpha and IL-6 by human HaCaT cells may potentially 
      classify skin sensitizers from non-sensitizers. Copyright (c) 2015 John Wiley & 
      Sons, Ltd.
CI  - Copyright (c) 2015 John Wiley & Sons, Ltd.
FAU - Jung, Daun
AU  - Jung D
AD  - Department of Microbiology and Immunology, and Institute of Endemic Disease, 
      College of Medicine, Seoul National University, Seoul, 110-799, South Korea.
FAU - Che, Jeong-Hwan
AU  - Che JH
AD  - Biomedical Research Institute, Seoul National University Hospital, Seoul, 
      110-744, Republic of Korea.
FAU - Lim, Kyung-Min
AU  - Lim KM
AD  - College of Pharmacology, Ewha Womans University, Seoul, 120-808, Republic of 
      Korea.
FAU - Chun, Young-Jin
AU  - Chun YJ
AD  - Chung-Ang University, College of Pharmacy, Seoul, 156-756, Republic of Korea.
FAU - Heo, Yong
AU  - Heo Y
AD  - Department of Occupational Health, College of Natural Sciences, Catholic 
      University of Daegu, Daegu, 712-702, South Korea.
FAU - Seok, Seung Hyeok
AU  - Seok SH
AD  - Department of Microbiology and Immunology, and Institute of Endemic Disease, 
      College of Medicine, Seoul National University, Seoul, 110-799, South Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151222
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
RN  - 0 (IL1A protein, human)
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-1alpha)
RN  - 0 (Interleukin-6)
RN  - 0 (Irritants)
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - 0 (Xenobiotics)
RN  - EUY85H477I (thiazolyl blue)
SB  - IM
MH  - Animal Testing Alternatives
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Dermatitis, Allergic Contact/pathology
MH  - Humans
MH  - Interleukin-1alpha/genetics/*metabolism
MH  - Interleukin-6/*metabolism
MH  - Irritants/toxicity
MH  - Keratinocytes/*drug effects/metabolism
MH  - Local Lymph Node Assay
MH  - Skin/cytology/*drug effects/metabolism
MH  - Tetrazolium Salts/metabolism
MH  - Thiazoles/metabolism
MH  - Xenobiotics/*toxicity
OTO - NOTNLM
OT  - HaCaT
OT  - cultured human keratinocyte
OT  - interleukin-1alpha
OT  - interleukin-6
OT  - skin sensitizer
EDAT- 2015/12/23 06:00
MHDA- 2017/04/11 06:00
CRDT- 2015/12/23 06:00
PHST- 2015/08/24 00:00 [received]
PHST- 2015/10/24 00:00 [revised]
PHST- 2015/11/06 00:00 [accepted]
PHST- 2015/12/23 06:00 [entrez]
PHST- 2015/12/23 06:00 [pubmed]
PHST- 2017/04/11 06:00 [medline]
AID - 10.1002/jat.3274 [doi]
PST - ppublish
SO  - J Appl Toxicol. 2016 Sep;36(9):1129-36. doi: 10.1002/jat.3274. Epub 2015 Dec 22.