PMID- 26692420
OWN - NLM
STAT- MEDLINE
DCOM- 20161021
LR  - 20190109
IS  - 1521-3773 (Electronic)
IS  - 1433-7851 (Print)
IS  - 1433-7851 (Linking)
VI  - 55
IP  - 5
DP  - 2016 Jan 26
TI  - Programming A Molecular Relay for Ultrasensitive Biodetection through 
      (129)Xe NMR.
PG  - 1733-6
LID - 10.1002/anie.201508990 [doi]
AB  - A supramolecular strategy for detecting specific proteins in complex media by 
      using hyperpolarized (129) Xe NMR is reported. A cucurbit[6]uril (CB[6])-based 
      molecular relay was programmed for three sequential equilibrium conditions by 
      designing a two-faced guest (TFG) that initially binds CB[6] and blocks the 
      CB[6]-Xe interaction. The protein analyte recruits the TFG and frees CB[6] for Xe 
      binding. TFGs containing CB[6]- and carbonic anhydrase II (CAII)-binding domains 
      were synthesized in one or two steps. X-ray crystallography confirmed TFG binding 
      to Zn(2+) in the deep CAII active-site cleft, which precludes simultaneous CB[6] 
      binding. The molecular relay was reprogrammed to detect avidin by using a 
      different TFG. Finally, Xe binding by CB[6] was detected in buffer and in E. coli 
      cultures expressing CAII through ultrasensitive (129) Xe NMR spectroscopy.
CI  - (c) 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Wang, Yanfei
AU  - Wang Y
AUID- ORCID: 0000-0002-9358-4759
AD  - Department of Chemistry, University of Pennsylvania, 231 South 34th Street, 
      Philadelphia, PA, 19104-6323, USA.
FAU - Roose, Benjamin W
AU  - Roose BW
AUID- ORCID: 0000-0003-3333-5053
AD  - Department of Chemistry, University of Pennsylvania, 231 South 34th Street, 
      Philadelphia, PA, 19104-6323, USA.
FAU - Philbin, John P
AU  - Philbin JP
AD  - Department of Chemistry, University of Pennsylvania, 231 South 34th Street, 
      Philadelphia, PA, 19104-6323, USA.
FAU - Doman, Jordan L
AU  - Doman JL
AD  - Department of Chemistry, University of Pennsylvania, 231 South 34th Street, 
      Philadelphia, PA, 19104-6323, USA.
FAU - Dmochowski, Ivan J
AU  - Dmochowski IJ
AUID- ORCID: 0000-0001-7162-1347
AD  - Department of Chemistry, University of Pennsylvania, 231 South 34th Street, 
      Philadelphia, PA, 19104-6323, USA. ivandmo@sas.upenn.edu.
LA  - eng
GR  - R01 GM097478/GM/NIGMS NIH HHS/United States
GR  - T32 GM008275/GM/NIGMS NIH HHS/United States
GR  - R01-GM097478/GM/NIGMS NIH HHS/United States
GR  - S10-DO016260/PHS HHS/International
GR  - P41 GM103403/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20151221
PL  - Germany
TA  - Angew Chem Int Ed Engl
JT  - Angewandte Chemie (International ed. in English)
JID - 0370543
RN  - 3H3U766W84 (Xenon)
SB  - IM
EIN - Angew Chem Int Ed Engl. 2016 Oct 10;55(42):12942. PMID: 27709821
MH  - Crystallography, X-Ray
MH  - Limit of Detection
MH  - Magnetic Resonance Spectroscopy/*methods
MH  - Xenon/*chemistry
PMC - PMC4902157
MID - NIHMS765627
OTO - NOTNLM
OT  - NMR spectroscopy
OT  - bioinorganic chemistry
OT  - biosensors
OT  - host-guest systems
OT  - molecular recognition
EDAT- 2015/12/23 06:00
MHDA- 2016/10/22 06:00
PMCR- 2017/01/26
CRDT- 2015/12/23 06:00
PHST- 2015/09/25 00:00 [received]
PHST- 2015/12/23 06:00 [entrez]
PHST- 2015/12/23 06:00 [pubmed]
PHST- 2016/10/22 06:00 [medline]
PHST- 2017/01/26 00:00 [pmc-release]
AID - 10.1002/anie.201508990 [doi]
PST - ppublish
SO  - Angew Chem Int Ed Engl. 2016 Jan 26;55(5):1733-6. doi: 10.1002/anie.201508990. 
      Epub 2015 Dec 21.