PMID- 26692941
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151222
LR  - 20220311
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 7
IP  - 10
DP  - 2015
TI  - MicroRNA-130a expression is decreased in Xinjiang Uygur patients with type 2 
      diabetes mellitus.
PG  - 1984-91
AB  - AIMS: MicroRNAs play important roles in energy metabolism, insulin synthesis, 
      insulin transport and the development of diabetes. This study aims to investigate 
      the expression and effect of microRNA-130a in Uygur patients with type 2 diabetes 
      mellitus (T2DM). MATERIALS AND METHODS: Peripheral blood and omental adipose 
      tissues were collected from individuals with normal glucose tolerance and 
      patients with T2DM. The microRNA expression profile of peripheral blood was 
      established by microarray analysis. The differentially expressed microRNAs and 
      possible target genes were identified by bioinformatics analysis. MicroRNA-130a 
      mimics and inhibitors were transfected into 3T3-L1 preadipocytes. RESULTS: Our 
      results showed that microRNA-130a expression level was significantly decreased in 
      peripheral blood and omental adipose tissues of T2DM patients (P < 0.01). 
      Peroxisome proliferator-activated receptors gamma (PPARgamma) were predicted as target 
      genes of microRNA-130a. This prediction was verified by the results that PPARgamma 
      mRNA expression in omental adipose tissues of T2DM patients were significantly 
      increased (P < 0.01). The glucose consumption level after microRNA-130a 
      transfection was significantly decreased (P < 0.05). And, microRNA-130a mimics 
      inhibited PPARgamma expression at both mRNA and protein level, further suggesting 
      that PPARgamma is a target gene of microRNA-130a. Additionally, adiponectin, 
      lipoprotein lipase, CCAAT enhancer binding protein alpha, and the downstream genes of 
      PPARgamma, were significantly decreased after microRNA-130a mimics transfection. 
      CONCLUSIONS: In conclusion, microRNA-130a is decreased in Uygur patients with 
      T2DM and it may play a role in T2DM through targeting PPARgamma.
FAU - Jiao, Yi
AU  - Jiao Y
AD  - Department of Biochemistry and Molecular Biology, Preclinical Medicine College, 
      Xinjiang Medical University Urumqi 830011, P. R China.
FAU - Zhu, Manli
AU  - Zhu M
AD  - Central Laboratory of Xinjiang Medical University, Xinjiang Medical University 
      Urumqi 830011, P. R China.
FAU - Mao, Xinmin
AU  - Mao X
AD  - Traditional Chinese Medical College, Xinjiang Medical University Urumqi 830011, 
      P. R China.
FAU - Long, Mei
AU  - Long M
AD  - Lab Center of Mechanism, Preclinical School, Xinjiang Medical University Urumqi 
      830011, P. R China.
FAU - Du, Xian
AU  - Du X
AD  - Department of Pharmacology, Preclinical Medicine College, Xinjiang Medical 
      University Urumqi, Xinjiang 830011, P. R China.
FAU - Wu, Yun
AU  - Wu Y
AD  - General Internal Medicine, First Affiliated Hospital of Xinjiang Medical 
      University Urumqi 830011, P. R China.
FAU - Abudureyimu, Kelimu
AU  - Abudureyimu K
AD  - Endoscopic Branch, Xinjiang Uygur Autonomous Regional People's Hospital Urumqi 
      830000, P. R China.
FAU - Zhang, Cheng
AU  - Zhang C
AD  - Endoscopic Branch, Xinjiang Uygur Autonomous Regional People's Hospital Urumqi 
      830000, P. R China.
FAU - Wang, Ye
AU  - Wang Y
AD  - Department of Pharmacology, Preclinical Medicine College, Xinjiang Medical 
      University Urumqi, Xinjiang 830011, P. R China.
FAU - Tao, Yicun
AU  - Tao Y
AD  - Department of Pharmacology, Preclinical Medicine College, Xinjiang Medical 
      University Urumqi, Xinjiang 830011, P. R China.
FAU - Luo, Xin
AU  - Luo X
AD  - Department of Pharmacology, Preclinical Medicine College, Xinjiang Medical 
      University Urumqi, Xinjiang 830011, P. R China.
FAU - Li, Linlin
AU  - Li L
AD  - Department of Pharmacology, Preclinical Medicine College, Xinjiang Medical 
      University Urumqi, Xinjiang 830011, P. R China.
LA  - eng
PT  - Journal Article
DEP - 20151015
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC4656774
OTO - NOTNLM
OT  - Uygur
OT  - microRNAs
OT  - microarray
OT  - peroxisome proliferator-activated receptors gamma
OT  - tumor necrosis factor alpha
OT  - type 2 diabetes
EDAT- 2015/12/23 06:00
MHDA- 2015/12/23 06:01
PMCR- 2015/10/15
CRDT- 2015/12/23 06:00
PHST- 2015/03/31 00:00 [received]
PHST- 2015/08/16 00:00 [accepted]
PHST- 2015/12/23 06:00 [entrez]
PHST- 2015/12/23 06:00 [pubmed]
PHST- 2015/12/23 06:01 [medline]
PHST- 2015/10/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2015 Oct 15;7(10):1984-91. eCollection 2015.