PMID- 26693067
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151222
LR  - 20220408
IS  - 2156-6976 (Print)
IS  - 2156-6976 (Electronic)
IS  - 2156-6976 (Linking)
VI  - 5
IP  - 10
DP  - 2015
TI  - The long noncoding RNA LINC01207 promotes proliferation of lung adenocarcinoma.
PG  - 3162-73
AB  - Lung adenocarcinoma (LAD) and lung squamous cell cancer (LSCC) are two most 
      common histological types of lung cancer, while they differ in many aspects. 
      Recent evidence shows that long non-coding RNAs (lncRNAs) play an important role 
      in the process of cancer initiation and progression. Thus, characterization of 
      LAD and LSCC associated lncRNAs may help understand the difference between LAD 
      and LSCC. Here, we analyzed three sets of RNA-seq data, including LAD RNA-seq 
      data from TCGA project. We identified a novel lncRNA, long intergenic non-protein 
      coding RNA 1207 (LINC01207) which was significantly up-regulated in LAD tissues 
      compared with paired non-tumor tissues (5.78 fold increase, P<0.05), while there 
      was no significant differences between LSCC tissues and adjacent non-tumor 
      tissues. The expression level of LINC01207 was associated with TNM stage of LAD 
      patients, and higher LINC01207 level indicated advanced TNM stage (P<0.05) and 
      shorter survival (HR=2.53, P<0.05). By small interfering RNA (siRNA) mediated 
      knockdown of LINC01207, we determined the biological function of LINC01207 in 
      A549 cell line. After knockdown of LINC01207, cell proliferation ability was 
      inhibited. Further analysis showed that after silence of LINC01207, the 
      percentage of apoptotic cells significantly increased. By RNA immunoprecipitation 
      and Chromatin immunoprecipitation assay, we demonstrated that LINC01207 could 
      bind with EZH2 and mediated trimethylation of histone 3 lysine 27 at the promoter 
      region of Bad, an important pro-apoptotic gene. Finally, we developed xenograft 
      tumor models in nude mice and xenograft tumors derived from A549 cells 
      transfected with siRNA-LINC01207 had significantly lower tumor weight and smaller 
      tumor volume. In summary, the novel lncRNA, LINC01207 is specifically 
      up-regulated in LAD but not in LSCC; and LINC01207 could promote LAD cell growth 
      both in vivo and in vitro.
FAU - Wang, Gongchao
AU  - Wang G
AD  - Department of Surgery, School of Nursing, Shandong University Jinan 250012, 
      China.
FAU - Chen, Hongbo
AU  - Chen H
AD  - Department of Surgery, School of Nursing, Shandong University Jinan 250012, 
      China.
FAU - Liu, Jun
AU  - Liu J
AD  - Department of Surgery, School of Nursing, Shandong University Jinan 250012, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20150915
PL  - United States
TA  - Am J Cancer Res
JT  - American journal of cancer research
JID - 101549944
PMC - PMC4656738
OTO - NOTNLM
OT  - LINC01207
OT  - Lung cancer
OT  - apoptosis
OT  - bad
OT  - lung adenocarcinoma
EDAT- 2015/12/23 06:00
MHDA- 2015/12/23 06:01
PMCR- 2015/09/15
CRDT- 2015/12/23 06:00
PHST- 2015/08/20 00:00 [received]
PHST- 2015/04/26 00:00 [accepted]
PHST- 2015/12/23 06:00 [entrez]
PHST- 2015/12/23 06:00 [pubmed]
PHST- 2015/12/23 06:01 [medline]
PHST- 2015/09/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Cancer Res. 2015 Sep 15;5(10):3162-73. eCollection 2015.