PMID- 26695310
OWN - NLM
STAT- MEDLINE
DCOM- 20160929
LR  - 20231213
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 146
DP  - 2016 Jan 1
TI  - Analysis of glyphosate and aminomethylphosphonic acid in leaves from Coffea 
      arabica using high performance liquid chromatography with quadrupole mass 
      spectrometry detection.
PG  - 609-20
LID - S0039-9140(15)30186-7 [pii]
LID - 10.1016/j.talanta.2015.07.059 [doi]
AB  - Glyphosate is a commonly applied herbicide in coffee plantations. Because of its 
      non-selective mode of action it can damage the crop exposed through spray drift. 
      Therefore, it is of interest to study glyphosate fate in coffee plants. The aim 
      of this study was to develop an analytical method for accurate and precise 
      quantification of glyphosate and its main metabolite aminomethylphosphonic acid 
      (AMPA) at trace levels in coffee leaves using liquid chromatography with 
      single-quadrupole mass spectrometry detection. The method is based on a two-step 
      solid phase extraction (SPE) with an intermediate derivatization reaction using 
      9-fluorenylmethylchloroformate (FMOC). An isotope dilution method was used to 
      account for matrix effects and to enhance the confidence in analyte 
      identification. The limit of quantification (LOQ) for glyphosate and AMPA in 
      coffee leaves was 41 and 111 mug kg(-1) dry weight, respectively. For the method 
      optimization a design of experiments (DOE) approach was used. The sample clean-up 
      procedure can be simplified for the analysis of less challenging matrices, for 
      laboratories having a tandem mass spectrometry detector and for cases in which 
      quantification limits above 0.1 mg kg(-1) are acceptable, which is often the case 
      for glyphosate. The method is robust, possesses high identification confidence, 
      while being suitable for most commercial and academic laboratories. All leaf 
      samples from five coffee fields analyzed (n=21) contained glyphosate, while AMPA 
      was absent. The simplified clean-up procedure was successfully validated for 
      coffee leaves, rice, black beans and river water.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Schrubbers, Lars C
AU  - Schrubbers LC
AD  - Department of Plant and Environmental Sciences, Faculty of Science, University of 
      Copenhagen, Frederiksberg C, Denmark. Electronic address: schruebbers@plen.ku.dk.
FAU - Masis-Mora, Mario
AU  - Masis-Mora M
AD  - Centro de Investigacion en Contaminacion Ambiental, Universidad de Costa Rica, 
      San Jose, Costa Rica.
FAU - Rojas, Elizabeth Carazo
AU  - Rojas EC
AD  - Centro de Investigacion en Contaminacion Ambiental, Universidad de Costa Rica, 
      San Jose, Costa Rica.
FAU - Valverde, Bernal E
AU  - Valverde BE
AD  - Department of Plant and Environmental Sciences, Faculty of Science, University of 
      Copenhagen, Frederiksberg C, Denmark; Investigacion y Desarrollo en Agricultura 
      Tropical S.A. (IDEA Tropical), Alajuela, Costa Rica.
FAU - Christensen, Jan H
AU  - Christensen JH
AD  - Department of Plant and Environmental Sciences, Faculty of Science, University of 
      Copenhagen, Frederiksberg C, Denmark.
FAU - Cedergreen, Nina
AU  - Cedergreen N
AD  - Department of Plant and Environmental Sciences, Faculty of Science, University of 
      Copenhagen, Frederiksberg C, Denmark.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150720
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Isoxazoles)
RN  - 0 (Organophosphonates)
RN  - 0 (Tetrazoles)
RN  - J090588E6X 
      (2-amino-3-(3-hydrox-5(2-methyl-2H-tetrazol-5-yl)isoxazol-4-yl)propionic acid)
RN  - TE7660XO1C (Glycine)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Coffea/*chemistry
MH  - Environment, Controlled
MH  - Glycine/*analogs & derivatives/analysis/chemistry/isolation & 
      purification/metabolism
MH  - Isoxazoles
MH  - Limit of Detection
MH  - Mass Spectrometry/*methods
MH  - Organophosphonates/*analysis/chemistry/isolation & purification/metabolism
MH  - Plant Leaves/*chemistry
MH  - Solid Phase Extraction
MH  - Tetrazoles
MH  - Glyphosate
OTO - NOTNLM
OT  - Coffea arabica
OT  - Coffee
OT  - Glyphosate analysis
OT  - Liquid chromatography mass spectrometry
OT  - Single quadrupole
EDAT- 2015/12/24 06:00
MHDA- 2016/09/30 06:00
CRDT- 2015/12/24 06:00
PHST- 2015/04/14 00:00 [received]
PHST- 2015/07/13 00:00 [revised]
PHST- 2015/07/19 00:00 [accepted]
PHST- 2015/12/24 06:00 [entrez]
PHST- 2015/12/24 06:00 [pubmed]
PHST- 2016/09/30 06:00 [medline]
AID - S0039-9140(15)30186-7 [pii]
AID - 10.1016/j.talanta.2015.07.059 [doi]
PST - ppublish
SO  - Talanta. 2016 Jan 1;146:609-20. doi: 10.1016/j.talanta.2015.07.059. Epub 2015 Jul 
      20.