PMID- 26695502
OWN - NLM
STAT- MEDLINE
DCOM- 20160603
LR  - 20181202
IS  - 1524-4636 (Electronic)
IS  - 1079-5642 (Linking)
VI  - 36
IP  - 2
DP  - 2016 Feb
TI  - Identification of an Amino Acid Residue Critical for Plasma Membrane Localization 
      of ATP-Binding Cassette Transporter G1--Brief Report.
PG  - 253-5
LID - 10.1161/ATVBAHA.115.306592 [doi]
AB  - OBJECTIVE: ATP-binding cassette transporter G1 (ABCG1) mediates cholesterol 
      efflux to lipidated lipoproteins. Conflicting data about cellular localization of 
      ABCG1 and its effect on cholesterol efflux have been reported. Here, we 
      investigated the underlying mechanisms for these different observations. APPROACH 
      AND RESULTS: Confocal microscopy and biotinylation were used to assess cell 
      surface localization of ABCG1. We found that mouse ABCG1 (mABCG1) used in one 
      previous study has a substitution of Leu to Pro at position 550 (mG1-L550P). When 
      the corresponding Leu at position 562 in human ABCG1 (hABCG1) was mutated to Pro 
      (hG1-L562P), the mutant hABCG1, like mG1-L550P, mainly resided intracellularly, 
      whereas wild-type mABCG1 and hABCG1 were localized on the plasma membrane. 
      However, replacement of this Leu with Pro had no significant effect on mABCG1- 
      and hABCG1-mediated cholesterol efflux. CONCLUSIONS: Leu at position 550/562 in 
      mABCG1/hABCG1 is critical for their plasma membrane localization but not for 
      ABCG1-mediated cholesterol efflux. Our findings indicate that the substitution of 
      Leu to Pro at position 550 in mABCG1 may contribute to the non-cell surface 
      localization of mABCG1 observed in the previous study.
CI  - (c) 2015 American Heart Association, Inc.
FAU - Gu, Hong-mei
AU  - Gu HM
AD  - From the Departments of Pediatrics and Biochemistry, Group on the Molecular and 
      Cell Biology of Lipids, University of Alberta, Alberta, Canada (H.-M.G., F.W., 
      A.A., S.D., D.-W.Z.); and Institute of Atherosclerosis in Taishan Medical 
      University, Taian, China (S.Q., D.-W.Z.).
FAU - Wang, Faqi
AU  - Wang F
AD  - From the Departments of Pediatrics and Biochemistry, Group on the Molecular and 
      Cell Biology of Lipids, University of Alberta, Alberta, Canada (H.-M.G., F.W., 
      A.A., S.D., D.-W.Z.); and Institute of Atherosclerosis in Taishan Medical 
      University, Taian, China (S.Q., D.-W.Z.).
FAU - Alabi, Adekunle
AU  - Alabi A
AD  - From the Departments of Pediatrics and Biochemistry, Group on the Molecular and 
      Cell Biology of Lipids, University of Alberta, Alberta, Canada (H.-M.G., F.W., 
      A.A., S.D., D.-W.Z.); and Institute of Atherosclerosis in Taishan Medical 
      University, Taian, China (S.Q., D.-W.Z.).
FAU - Deng, Shijun
AU  - Deng S
AD  - From the Departments of Pediatrics and Biochemistry, Group on the Molecular and 
      Cell Biology of Lipids, University of Alberta, Alberta, Canada (H.-M.G., F.W., 
      A.A., S.D., D.-W.Z.); and Institute of Atherosclerosis in Taishan Medical 
      University, Taian, China (S.Q., D.-W.Z.).
FAU - Qin, Shucun
AU  - Qin S
AD  - From the Departments of Pediatrics and Biochemistry, Group on the Molecular and 
      Cell Biology of Lipids, University of Alberta, Alberta, Canada (H.-M.G., F.W., 
      A.A., S.D., D.-W.Z.); and Institute of Atherosclerosis in Taishan Medical 
      University, Taian, China (S.Q., D.-W.Z.).
FAU - Zhang, Da-wei
AU  - Zhang DW
AD  - From the Departments of Pediatrics and Biochemistry, Group on the Molecular and 
      Cell Biology of Lipids, University of Alberta, Alberta, Canada (H.-M.G., F.W., 
      A.A., S.D., D.-W.Z.); and Institute of Atherosclerosis in Taishan Medical 
      University, Taian, China (S.Q., D.-W.Z.). dzhang@ualberta.ca.
LA  - eng
GR  - Canadian Institutes of Health Research/Canada
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151222
PL  - United States
TA  - Arterioscler Thromb Vasc Biol
JT  - Arteriosclerosis, thrombosis, and vascular biology
JID - 9505803
RN  - 0 (ABCG1 protein, human)
RN  - 0 (ABCG1 protein, mouse)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily G, Member 1)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Lipoproteins)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - GMW67QNF9C (Leucine)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily G, Member 1
MH  - ATP-Binding Cassette Transporters/genetics/*metabolism
MH  - Amino Acid Substitution
MH  - Animals
MH  - Cell Membrane/*metabolism
MH  - Cholesterol/*metabolism
MH  - Genotype
MH  - HEK293 Cells
MH  - Humans
MH  - Leucine
MH  - Lipoproteins/genetics/*metabolism
MH  - Mice
MH  - Microscopy, Confocal
MH  - Mutation
MH  - Phenotype
MH  - Transfection
OTO - NOTNLM
OT  - ATP-binding cassette transporter G1
OT  - cholesterol efflux
OT  - lipoprotein
OT  - mutation
OT  - protein trafficking
EDAT- 2015/12/24 06:00
MHDA- 2016/06/04 06:00
CRDT- 2015/12/24 06:00
PHST- 2015/09/15 00:00 [received]
PHST- 2015/12/13 00:00 [accepted]
PHST- 2015/12/24 06:00 [entrez]
PHST- 2015/12/24 06:00 [pubmed]
PHST- 2016/06/04 06:00 [medline]
AID - ATVBAHA.115.306592 [pii]
AID - 10.1161/ATVBAHA.115.306592 [doi]
PST - ppublish
SO  - Arterioscler Thromb Vasc Biol. 2016 Feb;36(2):253-5. doi: 
      10.1161/ATVBAHA.115.306592. Epub 2015 Dec 22.