PMID- 26696324
OWN - NLM
STAT- MEDLINE
DCOM- 20170926
LR  - 20181202
IS  - 1751-7370 (Electronic)
IS  - 1751-7362 (Print)
IS  - 1751-7362 (Linking)
VI  - 10
IP  - 7
DP  - 2016 Jul
TI  - Imaging the uptake of nitrogen-fixing bacteria into larvae of the coral Acropora 
      millepora.
PG  - 1804-8
LID - 10.1038/ismej.2015.229 [doi]
AB  - Diazotrophic bacteria are instrumental in generating biologically usable forms of 
      nitrogen by converting abundant dinitrogen gas (N2) into available forms, such as 
      ammonium. Although nitrogen is crucial for coral growth, direct observation of 
      associations between diazotrophs and corals has previously been elusive. We 
      applied fluorescence in situ hybridization (FISH) and nanoscale secondary ion 
      mass spectrometry to observe the uptake of (15)N-enriched diazotrophic Vibrio sp. 
      isolated from Acropora millepora into conspecific coral larvae. Incorporation of 
      Vibrio sp. cells was observed in coral larvae after 4-h incubation with enriched 
      bacteria. Uptake was restricted to the aboral epidermis of larvae, where Vibrio 
      cells clustered in elongated aggregations. Other bacterial associates were also 
      observed in epidermal areas in FISH analyses. Although the fate and role of these 
      bacteria requires additional investigation, this study describes a powerful 
      approach to further explore cell associations and nutritional pathways in the 
      early life stages of the coral holobiont.
FAU - Lema, Kimberley A
AU  - Lema KA
AD  - ARC Centre of Excellence for Coral Reef Studies, and College of Marine and 
      Environmental Sciences, James Cook University, Townsville, Queensland, Australia.
AD  - Centre for Marine Microbiology and Genetics, Australian Institute of Marine 
      Science, Townsville MC, Townsville, Queensland, Australia.
AD  - AIMS@JCU, James Cook University, Townsville, Queensland, Australia.
AD  - IFREMER, Dyneco Pelagos, Plouzane, France.
FAU - Clode, Peta L
AU  - Clode PL
AD  - Centre for Microscopy, Characterisation and Analysis, The University of Western 
      Australia, Stirling Hwy, Crawley, Western Australia, Australia.
AD  - The Oceans Institute, The University of Western Australia, Stirling Hwy, Crawley, 
      Western Australia, Australia.
FAU - Kilburn, Matt R
AU  - Kilburn MR
AD  - Centre for Microscopy, Characterisation and Analysis, The University of Western 
      Australia, Stirling Hwy, Crawley, Western Australia, Australia.
FAU - Thornton, Ruth
AU  - Thornton R
AD  - School of Paediatrics and Child Heath, The University of Western Australia, 
      Stirling Hwy, Crawley, Western Australia, Australia.
FAU - Willis, Bette L
AU  - Willis BL
AD  - ARC Centre of Excellence for Coral Reef Studies, and College of Marine and 
      Environmental Sciences, James Cook University, Townsville, Queensland, Australia.
AD  - AIMS@JCU, James Cook University, Townsville, Queensland, Australia.
FAU - Bourne, David G
AU  - Bourne DG
AD  - Centre for Marine Microbiology and Genetics, Australian Institute of Marine 
      Science, Townsville MC, Townsville, Queensland, Australia.
AD  - AIMS@JCU, James Cook University, Townsville, Queensland, Australia.
LA  - eng
PT  - Journal Article
DEP - 20151222
PL  - England
TA  - ISME J
JT  - The ISME journal
JID - 101301086
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Animals
MH  - Anthozoa/*microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - Larva
MH  - Nitrogen/*metabolism
MH  - Nitrogen Fixation
MH  - Nitrogen-Fixing Bacteria/*metabolism
PMC - PMC4918436
EDAT- 2015/12/24 06:00
MHDA- 2017/09/28 06:00
PMCR- 2017/07/01
CRDT- 2015/12/24 06:00
PHST- 2015/05/04 00:00 [received]
PHST- 2015/10/26 00:00 [revised]
PHST- 2015/11/06 00:00 [accepted]
PHST- 2015/12/24 06:00 [entrez]
PHST- 2015/12/24 06:00 [pubmed]
PHST- 2017/09/28 06:00 [medline]
PHST- 2017/07/01 00:00 [pmc-release]
AID - ismej2015229 [pii]
AID - 10.1038/ismej.2015.229 [doi]
PST - ppublish
SO  - ISME J. 2016 Jul;10(7):1804-8. doi: 10.1038/ismej.2015.229. Epub 2015 Dec 22.