PMID- 26702497
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20170804
IS  - 1873-3557 (Electronic)
IS  - 1386-1425 (Linking)
VI  - 157
DP  - 2016 Mar 15
TI  - Association of protein structure, protein and carbohydrate subfractions with 
      bioenergy profiles and biodegradation functions in modeled forage.
PG  - 265-270
LID - S1386-1425(15)30305-X [pii]
LID - 10.1016/j.saa.2015.11.017 [doi]
AB  - The objectives of this study were to detect unique aspects and association of 
      forage protein inherent structure, biological compounds, protein and carbohydrate 
      subfractions, bioenergy profiles, and biodegradation features. In this study, 
      common available alfalfa hay from two different sourced-origins (FSO vs. CSO) was 
      used as a modeled forage for inherent structure profile, bioenergy, 
      biodegradation and their association between their structure and bio-functions. 
      The molecular spectral profiles were determined using non-invasive molecular 
      spectroscopy. The parameters included: protein structure amide I group, amide II 
      group and their ratios; protein subfractions (PA1, PA2, PB1, PB2, PC); 
      carbohydrate fractions (CA1, CA2, CA3, CA4, CB1, CB2, CC); biodegradable and 
      undegradable fractions of protein (RDPA2, RDPB1, RDPB2, RDP; RUPA2 RUPB1, RUPB2, 
      RUPC, RUP); biodegradable and undegradable fractions of carbohydrate (RDCA4, 
      RDCB1, RDCB2, RDCB3, RDCHO; RUCA4, RUCB1; RUCB2; RUCB3 RUCC, RUCHO) and bioenergy 
      profiles (tdNDF, tdFA, tdCP, tdNFC, TDN1x, DE3x, ME3x, NEL3x; NEm, NEg). The 
      results show differences in protein and carbohydrate (CHO) subfractions in the 
      moderately degradable true protein fraction (PB1: 502 vs. 420 g/kg CP, P=0.09), 
      slowly degraded true protein fraction (PB2: 45 vs. 96 g/kg CP, P=0.02), 
      moderately degradable CHO fraction (CB2: 283 vs. 223 g/kg CHO, P=0.06) and slowly 
      degraded CHO fraction (CB3: 369 vs. 408 g/kg CHO) between the two sourced 
      origins. As to biodegradable (RD) fractions of protein and CHO in rumen, there 
      were differences in RD of PB1 (417 vs. 349 g/kg CP, P=0.09), RD of PB2 (29 vs. 62 
      g/kg CP, P=0.02), RD of CB2 (251 vs. 198 g/kg DM, P=0.06), RD of CB3 (236 vs. 261 
      g/kg CHO, P=0.08). As to bioenergy profile, there were differences in total 
      digestible nutrient (TDN: 551 vs. 537 g/kg DM, P=0.06), and metabolic bioenergy 
      (P=0.095). As to protein molecular structure, there were differences in protein 
      structure 1st and 2nd amide groups (P<0.10), but no difference in the 1st to 2nd 
      amide group intensity ratios (P>0.05). These results indicate that the 
      sourced-origins and the internal molecular structure profiles affected biological 
      functions, nutrient bioavailability and biodegradation.
CI  - Copyright (c) 2015. Published by Elsevier B.V.
FAU - Ji, Cuiying
AU  - Ji C
AD  - College of Animal Science and Veterinary Medicine, Tianjin Agricultural 
      University, 22 Jinjing Road, Tianjin 300384, China; Department of Animal and 
      Poultry Science, College of Agriculture and Bioresources, University of 
      Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada.
FAU - Zhang, Xuewei
AU  - Zhang X
AD  - College of Animal Science and Veterinary Medicine, Tianjin Agricultural 
      University, 22 Jinjing Road, Tianjin 300384, China. Electronic address: 
      zhangxuewei63@163.com.
FAU - Yu, Peiqiang
AU  - Yu P
AD  - College of Animal Science and Veterinary Medicine, Tianjin Agricultural 
      University, 22 Jinjing Road, Tianjin 300384, China; Department of Animal and 
      Poultry Science, College of Agriculture and Bioresources, University of 
      Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada. Electronic address: 
      peiqiang.yu@usask.ca.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151201
PL  - England
TA  - Spectrochim Acta A Mol Biomol Spectrosc
JT  - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
JID - 9602533
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Dietary Proteins)
RN  - 0 (Plant Proteins)
SB  - IM
MH  - Animal Feed/*analysis
MH  - Animals
MH  - Dietary Carbohydrates/analysis/*metabolism
MH  - Dietary Proteins/analysis/*metabolism
MH  - Digestion
MH  - Medicago sativa/chemistry/*metabolism
MH  - Plant Proteins/analysis/*metabolism
MH  - Rumen/*metabolism
OTO - NOTNLM
OT  - Association between structure and biofunctions
OT  - Biodegradation
OT  - Modeled forage
OT  - Molecular structure
EDAT- 2015/12/26 06:00
MHDA- 2016/12/15 06:00
CRDT- 2015/12/26 06:00
PHST- 2015/09/02 00:00 [received]
PHST- 2015/11/10 00:00 [revised]
PHST- 2015/11/18 00:00 [accepted]
PHST- 2015/12/26 06:00 [entrez]
PHST- 2015/12/26 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - S1386-1425(15)30305-X [pii]
AID - 10.1016/j.saa.2015.11.017 [doi]
PST - ppublish
SO  - Spectrochim Acta A Mol Biomol Spectrosc. 2016 Mar 15;157:265-270. doi: 
      10.1016/j.saa.2015.11.017. Epub 2015 Dec 1.