PMID- 26702561
OWN - NLM
STAT- MEDLINE
DCOM- 20161102
LR  - 20161230
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 49
DP  - 2016 Feb
TI  - Immune responses in the Japanese pufferfish (Takifugu rubripes) head kidney cells 
      stimulated with particulate silica.
PG  - 84-90
LID - S1050-4648(15)30275-8 [pii]
LID - 10.1016/j.fsi.2015.12.023 [doi]
AB  - Studies on immune response to crystal silica in mammals indicate immune 
      stimulation effect of environmental parameters including silica or asbestos, but 
      there is no information on this aspect in lower vertebrates. Therefore, we 
      examined expression of cytokine genes related to innate immunity in the Japanese 
      pufferfish, Fugu (Takifugu rubripes) head kidney (HK) cells stimulated with 
      particulate silica at 10 and 50 mug mL(-1). Expression of eleven cytokine genes 
      was analyzed by the multiplex RT-PCR method (GenomeLab Genetic Analysis System, 
      GeXPS; Beckman Coulter Inc.). Additionally, to confirm functionality of activated 
      inflammatory immunity, we assessed phagocytic activity. Expression of NLR family 
      genes as potential sensor molecules of inflammasome and inflammasome-associated 
      genes (ASC and caspase-1) was also confirmed in HK cells by quantitative 
      real-time PCR (qRT-PCR). As a result, an increased gene expression of 
      pro-inflammatory cytokines (IL-6, IL-17A/F3, TNF-alpha, TNF-beta and IFN-gamma) and other 
      cytokines (IL-4/13A, IL-4/13B, Type I-IFN) was recorded in particulate silica 
      stimulated HK cells. Moreover, phagocytic activity showed a tendency to 
      significantly increase in stimulated monocyte of HK cells after 6 h. Expression 
      of NLR-C9 and NLR-C12 genes significantly increased in silica-stimulated HK 
      cells. The particulate silica also significantly induced expression of 
      inflammosome-associated genes, which may relate to the induced NLR-Cs.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Morimoto, Takashi
AU  - Morimoto T
AD  - Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, 
      University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192, Japan.
FAU - Biswas, Gouranga
AU  - Biswas G
AD  - Interdisciplinary Graduate School of Agriculture and Engineering, University of 
      Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192, Japan.
FAU - Kono, Tomoya
AU  - Kono T
AD  - Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, 
      University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192, Japan.
FAU - Sakai, Masahiro
AU  - Sakai M
AD  - Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, 
      University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192, Japan.
FAU - Hikima, Jun-Ichi
AU  - Hikima J
AD  - Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, 
      University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192, Japan. 
      Electronic address: jhikima@cc.miyazaki-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151217
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Cytokines)
RN  - 0 (Fish Proteins)
RN  - 0 (Inflammasomes)
RN  - 7631-86-9 (Silicon Dioxide)
SB  - IM
MH  - Animals
MH  - Cytokines/genetics/metabolism
MH  - Fish Diseases/chemically induced/*immunology
MH  - Fish Proteins/*genetics/metabolism
MH  - *Gene Expression
MH  - Head Kidney/immunology/metabolism
MH  - Inflammasomes
MH  - Inflammation/chemically induced/immunology/*veterinary
MH  - Phagocytosis
MH  - Real-Time Polymerase Chain Reaction/veterinary
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Silicon Dioxide/*pharmacology
MH  - Takifugu/genetics/*immunology/metabolism
OTO - NOTNLM
OT  - ASC
OT  - Caspase-1
OT  - Inflammatory cytokines
OT  - Japanese pufferfish
OT  - Multiplex RT-PCR
OT  - Particulate silica
EDAT- 2015/12/26 06:00
MHDA- 2016/11/03 06:00
CRDT- 2015/12/26 06:00
PHST- 2015/08/07 00:00 [received]
PHST- 2015/12/12 00:00 [revised]
PHST- 2015/12/12 00:00 [accepted]
PHST- 2015/12/26 06:00 [entrez]
PHST- 2015/12/26 06:00 [pubmed]
PHST- 2016/11/03 06:00 [medline]
AID - S1050-4648(15)30275-8 [pii]
AID - 10.1016/j.fsi.2015.12.023 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2016 Feb;49:84-90. doi: 10.1016/j.fsi.2015.12.023. Epub 
      2015 Dec 17.