PMID- 26703450
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20161230
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 31
DP  - 2016 Feb
TI  - Inductive immune responses in the Japanese pufferfish (Takifugu rubripes) treated 
      with recombinant IFN-gamma, IFN-gammarel, IL-4/13A and IL-4/13B.
PG  - 50-6
LID - S1567-5769(15)30211-3 [pii]
LID - 10.1016/j.intimp.2015.12.005 [doi]
AB  - Immune regulation in response to recombinant cytokines (Th1- and Th2-types) is 
      poorly understood in fish. Therefore, we synthesized and purified the Japanese 
      pufferfish, (Takifugu rubripes) recombinant cytokines, namely interferon-gamma 
      (IFN-gamma), IFN-gammarel, interleukin (IL)-4/13A and IL-4/13B, and then administered by 
      injection to examine the immune regulatory effects on phagocytic activity, 
      superoxide anion production and lysozyme activity, and cytokine gene expressions 
      in treated fish head kidney (HK) at 1, 3 and 5 days post injection (dpi). 
      Pufferfish that received rIFN-gamma injection had an elevated phagocytic activity at 
      1 and 3 dpi, whereas rIFN-gammarel and rIL-4/13A treated fish showed an increased 
      phagocytic activity only at 1 and 3 dpi, respectively. Superoxide anion 
      production increased only at 1 dpi in rIFN-gamma, rIFN-gammarel and rIL-4/13A injected 
      pufferfish. Lysozyme showed a high activity at 1 dpi in rIFN-gamma injected fish, at 
      5 dpi in rIL-4/13A injected fish and all through the time course in rIL-4/13B 
      injected fish. rIFN-gamma stimulation caused an elevated IL-1beta and tumor necrosis 
      factor-alpha (TNF-alpha) transcriptions, whereas stimulation with rIFN-gammarel resulted 
      in IFN-gamma gene induction and a long term increase in IL-6 and IL-12p40 gene 
      expressions. Injection of rIL-4/13A induced transcription of IL-6, IL-12p40 and 
      TNF-alpha, while rIL-4/13B treatment enhanced IL-6, IL-12p35 and IL-12p40 gene 
      expressions. Our results suggest that each recombinant cytokine has a role in 
      activation of immune cells. More precisely, rIFN-gamma may be potentially the most 
      effective immune inducer among the tested cytokines. Therefore, use of 
      recombinant Th1 and Th2 cytokines as immune enhancers could be an efficient 
      technique for disease prevention in fish.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Biswas, Gouranga
AU  - Biswas G
AD  - Interdisciplinary Graduate School of Agriculture and Engineering, University of 
      Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192, Japan.
FAU - Nagamine, Ryusuke
AU  - Nagamine R
AD  - Interdisciplinary Graduate School of Agriculture and Engineering, University of 
      Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192, Japan.
FAU - Hikima, Jun-ichi
AU  - Hikima J
AD  - Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, 
      University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192, Japan.
FAU - Sakai, Masahiro
AU  - Sakai M
AD  - Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, 
      University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192, Japan.
FAU - Kono, Tomoya
AU  - Kono T
AD  - Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, 
      University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192, Japan. 
      Electronic address: tkono@cc.miyazaki-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151217
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Fish Proteins)
RN  - 0 (Interleukin-13)
RN  - 0 (Recombinant Proteins)
RN  - 11062-77-4 (Superoxides)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 3.2.1.17 (Muramidase)
SB  - IM
MH  - Animals
MH  - Fish Proteins/genetics/*immunology
MH  - Gene Expression
MH  - Head Kidney/*immunology
MH  - Interferon-gamma/genetics/*immunology
MH  - Interleukin-13/genetics/*immunology
MH  - Interleukin-4/genetics/*immunology
MH  - Lymphocyte Activation
MH  - Muramidase/metabolism
MH  - Oxidative Stress
MH  - Phagocytosis/immunology
MH  - Protein Engineering
MH  - Recombinant Proteins/genetics/*immunology
MH  - Superoxides/metabolism
MH  - Takifugu/*immunology
OTO - NOTNLM
OT  - IFN-gamma and IFN-gammarel
OT  - IL-4/13A and IL-4/13B
OT  - Immune regulation
OT  - Recombinant cytokines
OT  - Th1 cytokines
OT  - Th2 cytokines
EDAT- 2015/12/26 06:00
MHDA- 2016/12/15 06:00
CRDT- 2015/12/26 06:00
PHST- 2015/08/05 00:00 [received]
PHST- 2015/11/18 00:00 [revised]
PHST- 2015/12/02 00:00 [accepted]
PHST- 2015/12/26 06:00 [entrez]
PHST- 2015/12/26 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - S1567-5769(15)30211-3 [pii]
AID - 10.1016/j.intimp.2015.12.005 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2016 Feb;31:50-6. doi: 10.1016/j.intimp.2015.12.005. Epub 
      2015 Dec 17.