PMID- 26707356
OWN - NLM
STAT- MEDLINE
DCOM- 20171116
LR  - 20220317
IS  - 1478-3231 (Electronic)
IS  - 1478-3223 (Linking)
VI  - 36
IP  - 7
DP  - 2016 Jul
TI  - A strong conservative tendency in HBV transcriptase (RT): a majority of natural 
      RT mutations derived from the S gene.
PG  - 963-70
LID - 10.1111/liv.13051 [doi]
AB  - BACKGROUND & AIMS: Little is known about natural mutations in the HBV reverse 
      transcriptase (RT) region. Our study aimed to characterize the natural RT 
      mutation along the natural course of chronic Hepatitis B (CHB). METHODS: Sixty 
      CHB patients (immune-tolerant phase, IT, n = 20; immune-active phase, IA, n = 20 
      and inactive carriers phase, IC, n = 20) were selected from the Focal study, 
      including 25 subjects with median 18 months follow-up. Mutations were evaluated 
      at both RT and main S protein encoding region by clone-based sequencing. RESULTS: 
      The HBV RT quasispecies had significant lower heterogeneity in IT than IA and IC 
      phases (P < 0.05), but not between IA and IC phases (P > 0.05). Limited 
      heterogeneity over time was further confirmed in a longitudinal study. Locations 
      of RT mutations were primarily located in the interdomians and the lowest in 
      functional domains in each phase. Mutations in human leukocyte antigen (HLA) I 
      epitopes (IT, 0.95%; IA, 1.31%; IC, 1.28%, P < 0.05) and HLA II epitopes (IT, 
      0.70%; IA, 0.90%; IC, 1.45%, P < 0.01) varied significantly over time. More 
      frequent mutations were detected in the ORF of S gene from the same clones (HBsAg 
      vs. RT: IT, 75 vs. 45; IA, 83 vs. 64; IC, 80 vs. 65). The majority of RT 
      mutations were shared with genetic changes in the main S gene. CONCLUSIONS: Our 
      findings suggested that HBV RT showed a strong conservative tendency and a 
      majority of their natural mutations were derived from the same genetic changes in 
      the S gene.
CI  - (c) 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Li, Hu
AU  - Li H
AD  - Department of Infectious Diseases, Institute for Viral Hepatitis, Key Laboratory 
      of Molecular Biology for Infectious Diseases, Ministry of Education, Second 
      Affiliated Hospital of Chongqing Medical University, Chongqing, China.
FAU - Song, Xiao-Fei
AU  - Song XF
AD  - Department of Infectious Diseases, Institute for Viral Hepatitis, Key Laboratory 
      of Molecular Biology for Infectious Diseases, Ministry of Education, Second 
      Affiliated Hospital of Chongqing Medical University, Chongqing, China.
FAU - Hu, Ting-Ting
AU  - Hu TT
AD  - Department of Infectious Diseases, Institute for Viral Hepatitis, Key Laboratory 
      of Molecular Biology for Infectious Diseases, Ministry of Education, Second 
      Affiliated Hospital of Chongqing Medical University, Chongqing, China.
FAU - Ren, Hong
AU  - Ren H
AD  - Department of Infectious Diseases, Institute for Viral Hepatitis, Key Laboratory 
      of Molecular Biology for Infectious Diseases, Ministry of Education, Second 
      Affiliated Hospital of Chongqing Medical University, Chongqing, China.
FAU - Hu, Peng
AU  - Hu P
AD  - Department of Infectious Diseases, Institute for Viral Hepatitis, Key Laboratory 
      of Molecular Biology for Infectious Diseases, Ministry of Education, Second 
      Affiliated Hospital of Chongqing Medical University, Chongqing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160108
PL  - United States
TA  - Liver Int
JT  - Liver international : official journal of the International Association for the 
      Study of the Liver
JID - 101160857
RN  - 0 (DNA, Viral)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - Adult
MH  - China
MH  - DNA, Viral/*genetics
MH  - Female
MH  - Genetic Heterogeneity
MH  - Genotype
MH  - Hepatitis B virus/enzymology/*genetics
MH  - Hepatitis B, Chronic/*virology
MH  - Humans
MH  - Longitudinal Studies
MH  - Male
MH  - Mutation
MH  - RNA-Directed DNA Polymerase/*genetics
MH  - Young Adult
OTO - NOTNLM
OT  - Mutation
OT  - chronic HBV infection
OT  - chronic hepatitis B
OT  - reverse transcriptase
EDAT- 2015/12/29 06:00
MHDA- 2017/11/29 06:00
CRDT- 2015/12/29 06:00
PHST- 2015/05/22 00:00 [received]
PHST- 2015/12/11 00:00 [accepted]
PHST- 2015/12/29 06:00 [entrez]
PHST- 2015/12/29 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
AID - 10.1111/liv.13051 [doi]
PST - ppublish
SO  - Liver Int. 2016 Jul;36(7):963-70. doi: 10.1111/liv.13051. Epub 2016 Jan 8.