PMID- 26707636
OWN - NLM
STAT- MEDLINE
DCOM- 20161221
LR  - 20220330
IS  - 1532-2785 (Electronic)
IS  - 0143-4179 (Linking)
VI  - 56
DP  - 2016 Apr
TI  - Neuropeptide Y stimulates osteoblastic differentiation and VEGF expression of 
      bone marrow mesenchymal stem cells related to canonical Wnt signaling activating 
      in vitro.
PG  - 105-13
LID - S0143-4179(15)00228-0 [pii]
LID - 10.1016/j.npep.2015.12.008 [doi]
AB  - Neuropeptide Y (NPY) is a neuropeptide secreted by sensory nerve fibers 
      distributed in the marrow and vascular canals of bone tissue. However, the effect 
      of NPY on the osteogenic ability of bone marrow mesenchymal stem cells (BMSCs) 
      remains controversial and has not been thoroughly investigated. To explore the 
      osteogenic activity and the migration and VEGF expression capabilities of BMSCs 
      affected by NPY, as well as the underlying mechanisms, we investigated the 
      potential relationships among NPY, osteoblastic differentiation, angiogenesis and 
      canonical Wnt signaling in BMSCs. NPY was observed to regulate osteoblastic 
      differentiation at concentrations ranging from 10(-8) to 10(-12)mol/L, and the 
      effects of NPY on the levels of Wnt signaling proteins were detected using 
      Western blotting. To unravel the underlying mechanism, BMSCs were treated with 
      NPY after pretreatment with the NPY-1R antagonist PD160170 or the Wnt pathway 
      antagonist DKK1, and gene expression levels of Wnt signaling molecules and 
      osteoblastic markers were determined by qPCR. Our results indicated that NPY 
      significantly promoted osteoblastic differentiation of BMSCs in a 
      concentration-dependent manner and up-regulated the expression levels of proteins 
      including beta-catenin and p-GSK-3beta and the mRNA level of beta-catenin. Moreover, NPY 
      promoted the translocation of beta-catenin into nucleus. The effects of NPY were 
      inhibited by PD160170 or DKK1. Additionally, NPY enhanced the ability of BMSCs to 
      migrate and promoted the expression of vascular endothelial growth factor (VEGF) 
      as measured by immunocytochemical staining, qPCR and Western blot. These results 
      suggested that NPY may stimulate osteoblastic differentiation via activating 
      canonical Wnt signaling and enhance the angiogenic capacity of BMSCs.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Liu, Song
AU  - Liu S
AD  - Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical 
      University, 1838 North Guangzhou Avenue, Guangzhou City, Guangdong Province 
      510515, People's Republic of China.
FAU - Jin, Dan
AU  - Jin D
AD  - Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical 
      University, 1838 North Guangzhou Avenue, Guangzhou City, Guangdong Province 
      510515, People's Republic of China. Electronic address: nfjindan@126.com.
FAU - Wu, Jian-qun
AU  - Wu JQ
AD  - Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical 
      University, 1838 North Guangzhou Avenue, Guangzhou City, Guangdong Province 
      510515, People's Republic of China.
FAU - Xu, Zi-yi
AU  - Xu ZY
AD  - Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical 
      University, 1838 North Guangzhou Avenue, Guangzhou City, Guangdong Province 
      510515, People's Republic of China.
FAU - Fu, Su
AU  - Fu S
AD  - Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical 
      University, 1838 North Guangzhou Avenue, Guangzhou City, Guangdong Province 
      510515, People's Republic of China.
FAU - Mei, Gang
AU  - Mei G
AD  - Department of Orthopaedics, Xiangyang Central Hospital, Xiangyang City, Hubei 
      Province 441021, People's Republic of China.
FAU - Zou, Zhen-Lv
AU  - Zou ZL
AD  - Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical 
      University, 1838 North Guangzhou Avenue, Guangzhou City, Guangdong Province 
      510515, People's Republic of China.
FAU - Ma, Sheng-hui
AU  - Ma SH
AD  - Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical 
      University, 1838 North Guangzhou Avenue, Guangzhou City, Guangdong Province 
      510515, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151211
PL  - Netherlands
TA  - Neuropeptides
JT  - Neuropeptides
JID - 8103156
RN  - 0 (Bmp2 protein, rat)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Neuropeptide Y)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (vascular endothelial growth factor A, rat)
SB  - IM
MH  - Animals
MH  - Bone Morphogenetic Protein 2/metabolism
MH  - *Cell Differentiation/drug effects
MH  - Cell Movement/drug effects
MH  - Cells, Cultured
MH  - In Vitro Techniques
MH  - Male
MH  - Mesenchymal Stem Cells/drug effects/metabolism/*physiology
MH  - Neuropeptide Y/administration & dosage/*physiology
MH  - Osteoblasts/drug effects/*physiology
MH  - Rats
MH  - Vascular Endothelial Growth Factor A/*metabolism
MH  - Wnt Signaling Pathway/drug effects
OTO - NOTNLM
OT  - BMSCs
OT  - Differentiation
OT  - Neuropeptide Y
OT  - VEGF
OT  - Wnt signaling
EDAT- 2015/12/29 06:00
MHDA- 2016/12/22 06:00
CRDT- 2015/12/29 06:00
PHST- 2015/07/05 00:00 [received]
PHST- 2015/12/09 00:00 [revised]
PHST- 2015/12/10 00:00 [accepted]
PHST- 2015/12/29 06:00 [entrez]
PHST- 2015/12/29 06:00 [pubmed]
PHST- 2016/12/22 06:00 [medline]
AID - S0143-4179(15)00228-0 [pii]
AID - 10.1016/j.npep.2015.12.008 [doi]
PST - ppublish
SO  - Neuropeptides. 2016 Apr;56:105-13. doi: 10.1016/j.npep.2015.12.008. Epub 2015 Dec 
      11.