PMID- 26708340
OWN - NLM
STAT- MEDLINE
DCOM- 20161020
LR  - 20161230
IS  - 1872-7654 (Electronic)
IS  - 0301-2115 (Linking)
VI  - 196
DP  - 2016 Jan
TI  - Placental NRP1 and VEGF expression in pre-eclamptic women and in a 
      homocysteine-treated mouse model of pre-eclampsia.
PG  - 69-75
LID - S0301-2115(15)00408-X [pii]
LID - 10.1016/j.ejogrb.2015.11.017 [doi]
AB  - OBJECTIVES: To investigate the expression levels of neuropilin 1 (NRP1) and 
      vascular endothelial growth factor (VEGF) in the placentas of women with 
      pre-eclampsia (PE), determine whether homocysteine (Hcy) contributes to the 
      development of PE in mice, and detect alterations in placental NRP1 and VEGF in 
      Hcy-treated mice. STUDY DESIGN: Placental tissue samples were obtained from 16 
      patients with and without PE. Using reverse transcription polymerase chain 
      reaction (RT-PCR) and western blot analysis, the expression levels of NRP1 and 
      VEGF in PE and control placental tissues were examined. Immunohistochemical (IHC) 
      assay was used to detect the localization of NRP1 and VEGF proteins. Pregnant 
      mice were treated with DL-Hcy on embryonic day 7.5. The symptoms of the treated 
      mice were examined. The expression levels of Ddah1, Ddah2, eNos, Cbs and Cse were 
      determined by quantitative RT-PCR to study the possible mechanism of 
      hyperhomocysteinemia (HHcy). Nrp1 and Vegf expression levels in the placentas of 
      treated mice were measured by quantitative RT-PCR, western blot analysis and IHC. 
      RESULTS: NRP1 and VEGF were expressed at lower levels in women with PE compared 
      with control women. The immunoreactivity of NRP1 was detected in villous 
      trophoblast cells and villous capillary endothelial cells (ECs). Immunoreactive 
      VEGF was observed mainly in vascular ECs within the villi. The pregnant mice 
      treated with DL-Hcy showed PE-like symptoms such as higher systolic blood 
      pressure and proteinuria in late pregnancy. Compared with the control mice, 
      Ddah1, Ddah2 and eNos mRNA were expressed at lower levels, and Cbs and Cse mRNA 
      were expressed at significantly higher levels in the placentas of the Hcy-treated 
      group. Expression levels of Nrp1 and Vegf in mice placentas were decreased in the 
      Hcy-treated group. CONCLUSIONS: DL-Hcy can induce PE-like symptoms in mice. Both 
      placental NRP1 and VEGF were expressed at lower levels in women with PE and 
      Hcy-treated mice, which may contribute to endothelial damage.
CI  - Copyright (c) 2015 Elsevier Ireland Ltd. All rights reserved.
FAU - Xu, X
AU  - Xu X
AD  - Department of Obstetrics and Gynaecology, International Peace Maternity and Child 
      Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, PR 
      China.
FAU - Yang, X-Y
AU  - Yang XY
AD  - Department of Obstetrics and Gynaecology, International Peace Maternity and Child 
      Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, PR 
      China; Institute of Embryo-Fetal Original Adult Disease Affiliated to Shanghai 
      Jiaotong University School of Medicine, Shanghai, PR China.
FAU - He, B-W
AU  - He BW
AD  - Department of Obstetrics and Gynaecology, International Peace Maternity and Child 
      Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, PR 
      China.
FAU - Yang, W-J
AU  - Yang WJ
AD  - Department of Obstetrics and Gynaecology, International Peace Maternity and Child 
      Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, PR 
      China.
FAU - Cheng, W-W
AU  - Cheng WW
AD  - Department of Obstetrics and Gynaecology, International Peace Maternity and Child 
      Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, PR 
      China. Electronic address: wwcheng29@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151124
PL  - Ireland
TA  - Eur J Obstet Gynecol Reprod Biol
JT  - European journal of obstetrics, gynecology, and reproductive biology
JID - 0375672
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 144713-63-3 (Neuropilin-1)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Disease Models, Animal
MH  - Female
MH  - Homocysteine
MH  - Humans
MH  - Hyperhomocysteinemia/metabolism
MH  - Mice
MH  - Neuropilin-1/*metabolism
MH  - Placenta/*metabolism
MH  - Pre-Eclampsia/chemically induced/*metabolism
MH  - Pregnancy
MH  - Vascular Endothelial Growth Factor A/*metabolism
OTO - NOTNLM
OT  - Homocysteine
OT  - Neuropilin 1
OT  - Pre-eclampsia
OT  - Vascular endothelial growth factor
EDAT- 2015/12/29 06:00
MHDA- 2016/10/21 06:00
CRDT- 2015/12/29 06:00
PHST- 2015/02/04 00:00 [received]
PHST- 2015/06/20 00:00 [revised]
PHST- 2015/11/19 00:00 [accepted]
PHST- 2015/12/29 06:00 [entrez]
PHST- 2015/12/29 06:00 [pubmed]
PHST- 2016/10/21 06:00 [medline]
AID - S0301-2115(15)00408-X [pii]
AID - 10.1016/j.ejogrb.2015.11.017 [doi]
PST - ppublish
SO  - Eur J Obstet Gynecol Reprod Biol. 2016 Jan;196:69-75. doi: 
      10.1016/j.ejogrb.2015.11.017. Epub 2015 Nov 24.