PMID- 26709097
OWN - NLM
STAT- MEDLINE
DCOM- 20160915
LR  - 20220409
IS  - 1930-739X (Electronic)
IS  - 1930-7381 (Linking)
VI  - 24
IP  - 2
DP  - 2016 Feb
TI  - Tumor suppressor RIZ1 in obesity and the PI3K/AKT/mTOR pathway.
PG  - 389-97
LID - 10.1002/oby.21364 [doi]
AB  - OBJECTIVE: The aim of this study was to investigate the shared molecular pathways 
      of obesity and cancer by exploring the role of RIZ1 in obesity and the 
      phospatidylinositol 3-kinase (PI3K)/V-Akt murine thymoma viral oncogene homolog 
      (PKB) (AKT)/mechanistic target of rapamycin (mTOR) pathway. METHODS: Male wild 
      type (WT) and Riz1(-/-) mice (KO) were fed a standard diet (STD) or a high-fat 
      (HF) diet for up to 8 months. These mice were studied for phenotypic and 
      molecular changes. RESULTS: Riz1(-/-) mice gained more weight on a HF diet 
      compared to WT mice, with higher free fatty acid and increased visceral fat. 
      Metabolic cage analysis of Riz1(-/-) mice showed lower oxygen consumption but no 
      changes in food intake and ambulatory activity. Riz1(-/-) mice showed impaired 
      glucose regulation but no change in insulin sensitivity. RNA-seq and quantitative 
      RT-PCR analysis found altered expression in certain glycolysis and ATP production 
      genes such as Ubiad1, Atp5g2, and Cyp4a12. The PI3K/AKT/mTOR pathway was 
      activated in the Riz1(-/-) mice fed a HF diet with higher Akt3 mRNA levels and 
      increased phosphorylation of AKT (Ser473), mTOR, and S6. CONCLUSIONS: The results 
      identify RIZ1 as an important regulator of both Akt3 transcription and AKT 
      phosphorylation and suggest a role for RIZ1 in HF-induced obesity and the AKT 
      pathway.
CI  - (c) 2015 The Obesity Society.
FAU - Xie, Xiaolei
AU  - Xie X
AD  - State Key Lab of Medical Genetics, Xiangya Medical School, Central South 
      University, Changsha, China.
FAU - Man, Xian
AU  - Man X
AD  - State Key Lab of Medical Genetics, Xiangya Medical School, Central South 
      University, Changsha, China.
FAU - Zhu, Zuobin
AU  - Zhu Z
AD  - State Key Lab of Medical Genetics, Xiangya Medical School, Central South 
      University, Changsha, China.
FAU - Yuan, Dejian
AU  - Yuan D
AD  - State Key Lab of Medical Genetics, Xiangya Medical School, Central South 
      University, Changsha, China.
FAU - Huang, Shi
AU  - Huang S
AD  - State Key Lab of Medical Genetics, Xiangya Medical School, Central South 
      University, Changsha, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151226
PL  - United States
TA  - Obesity (Silver Spring)
JT  - Obesity (Silver Spring, Md.)
JID - 101264860
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Multiprotein Complexes)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
RN  - EC 2.1.1.43 (PRDM2 protein, human)
RN  - EC 2.7.11.1 (Mechanistic Target of Rapamycin Complex 1)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - DNA-Binding Proteins/*genetics
MH  - Gene Expression Profiling
MH  - Histone-Lysine N-Methyltransferase/*genetics
MH  - Insulin Resistance/*genetics
MH  - Male
MH  - Mechanistic Target of Rapamycin Complex 1
MH  - Mice
MH  - Multiprotein Complexes/genetics
MH  - Nuclear Proteins/*genetics
MH  - Obesity/*genetics/metabolism
MH  - Random Allocation
MH  - TOR Serine-Threonine Kinases/genetics
MH  - Transcription Factors/*genetics
EDAT- 2015/12/29 06:00
MHDA- 2016/09/16 06:00
CRDT- 2015/12/29 06:00
PHST- 2015/07/23 00:00 [received]
PHST- 2015/09/17 00:00 [revised]
PHST- 2015/09/22 00:00 [accepted]
PHST- 2015/12/29 06:00 [entrez]
PHST- 2015/12/29 06:00 [pubmed]
PHST- 2016/09/16 06:00 [medline]
AID - 10.1002/oby.21364 [doi]
PST - ppublish
SO  - Obesity (Silver Spring). 2016 Feb;24(2):389-97. doi: 10.1002/oby.21364. Epub 2015 
      Dec 26.