PMID- 26709732
OWN - NLM
STAT- MEDLINE
DCOM- 20170807
LR  - 20190117
IS  - 1471-4159 (Electronic)
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 141
IP  - 4
DP  - 2017 May
TI  - Effects of cold temperatures on the excitability of rat trigeminal ganglion 
      neurons that are not for cold sensing.
PG  - 532-543
LID - 10.1111/jnc.13511 [doi]
AB  - Aside from a small population of primary afferent neurons for sensing cold, which 
      generate sensations of innocuous and noxious cold, it is generally believed that 
      cold temperatures suppress the excitability of primary afferent neurons not 
      responsible for cold sensing. These not-for-cold-sensing neurons include the 
      majority of non-nociceptive and nociceptive afferent neurons. In this study we 
      have found that the not-for-cold-sensing neurons of rat trigeminal ganglia (TG) 
      change their excitability in several ways at cooling temperatures. In nearly 70% 
      of not-for-cold-sensing TG neurons, a cooling temperature of 15 degrees C increases their 
      membrane excitability. We regard these neurons as cold-active neurons. For the 
      remaining 30% of not-for-cold-sensing TG neurons, the cooling temperature of 15 degrees C 
      either has no effect (cold-ineffective neurons) or suppress their membrane 
      excitability (cold-suppressive neurons). For cold-active neurons, the cold 
      temperature of 15 degrees C increases their excitability as is evidenced by increases in 
      action potential (AP) firing numbers and/or the reduction in AP rheobase when 
      these neurons are depolarized electrically. The cold temperature of 15 degrees C 
      significantly inhibits M-currents and increases membrane input resistance of 
      cold-active neurons. Retigabine, an M-current activator, abolishes the effect of 
      cold temperatures on AP firing, but not the effect of cold temperature on AP 
      rheobase levels. The inhibition of M-currents and the increases of membrane input 
      resistance are likely two mechanisms by which cooling temperatures increase the 
      excitability of not-for-cold-sensing TG neurons. This article is part of the 
      special article series "Pain".
CI  - (c) 2015 International Society for Neurochemistry.
FAU - Kanda, Hirosato
AU  - Kanda H
AD  - Department of Anesthesiology and Perioperative Medicine, College of Medicine, 
      University of Alabama at Birmingham, Birmingham, Alabama, USA.
FAU - Gu, Jianguo G
AU  - Gu JG
AD  - Department of Anesthesiology and Perioperative Medicine, College of Medicine, 
      University of Alabama at Birmingham, Birmingham, Alabama, USA.
LA  - eng
GR  - R01 DE018661/DE/NIDCR NIH HHS/United States
GR  - R01 DE023090/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20170222
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Carbamates)
RN  - 0 (Membrane Transport Modulators)
RN  - 0 (Phenylenediamines)
RN  - 0 (Potassium Channels)
RN  - 0 (TRPM Cation Channels)
RN  - 0 (Trpm8 protein, rat)
RN  - 12G01I6BBU (ezogabine)
SB  - IM
MH  - Action Potentials/physiology
MH  - Animals
MH  - Carbamates/pharmacology
MH  - Cell Membrane/physiology
MH  - *Cold Temperature
MH  - In Vitro Techniques
MH  - Membrane Transport Modulators/pharmacology
MH  - Neurons/drug effects/*physiology
MH  - Nociceptors/drug effects/physiology
MH  - Patch-Clamp Techniques
MH  - Phenylenediamines/pharmacology
MH  - Potassium Channels/drug effects/genetics/physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sensory Receptor Cells
MH  - TRPM Cation Channels/genetics/physiology
MH  - Thermosensing/*drug effects
MH  - Trigeminal Ganglion/cytology/drug effects/*physiology
PMC - PMC4925314
MID - NIHMS783494
OTO - NOTNLM
OT  - KCNQ channels
OT  - M-currents
OT  - cold
OT  - pain
OT  - retigabine
OT  - trigeminal ganglion neurons
EDAT- 2015/12/29 06:00
MHDA- 2017/08/08 06:00
PMCR- 2017/05/04
CRDT- 2015/12/29 06:00
PHST- 2015/10/13 00:00 [received]
PHST- 2015/12/01 00:00 [revised]
PHST- 2015/12/02 00:00 [accepted]
PHST- 2015/12/29 06:00 [pubmed]
PHST- 2017/08/08 06:00 [medline]
PHST- 2015/12/29 06:00 [entrez]
PHST- 2017/05/04 00:00 [pmc-release]
AID - 10.1111/jnc.13511 [doi]
PST - ppublish
SO  - J Neurochem. 2017 May;141(4):532-543. doi: 10.1111/jnc.13511. Epub 2017 Feb 22.