PMID- 26710682
OWN - NLM
STAT- MEDLINE
DCOM- 20171107
LR  - 20171107
IS  - 1520-4812 (Electronic)
IS  - 1043-1802 (Linking)
VI  - 27
IP  - 1
DP  - 2016 Jan 20
TI  - Synergistic Effect of Detection and Separation for Pathogen Using Magnetic 
      Clusters.
PG  - 59-65
LID - 10.1021/acs.bioconjchem.5b00681 [doi]
AB  - Early diagnosis of infectious diseases is important for treatment; therefore, 
      selective and rapid detection of pathogenic bacteria is essential for human 
      health. We report a strategy for highly selective detection and rapid separation 
      of pathogenic microorganisms using magnetic nanoparticle clusters. Our approach 
      to develop probes for pathogenic bacteria, including Salmonella, is based on a 
      theoretically optimized model for the size of clustered magnetic nanoparticles. 
      The clusters were modified to provide enhanced aqueous solubility and versatile 
      conjugation sites for antibody immobilization. The clusters with the desired 
      magnetic property were then prepared at critical micelle concentration (CMC) by 
      evaporation-induced self-assembly (EISA). Two different types of target-specific 
      antibodies for H- and O-antigens were incorporated on the cluster surface for 
      selective binding to biological compartments of the flagella and cell body, 
      respectively. For the two different specific binding properties, Salmonella were 
      effectively captured with the O-antibody-coated polysorbate 80-coated magnetic 
      nanoclusters (PCMNCs). The synergistic effect of combining selective targeting 
      and the clustered magnetic probe leads to both selective and rapid detection of 
      infectious pathogens.
FAU - Kim, Yong-Tae
AU  - Kim YT
FAU - Kim, Kook-Han
AU  - Kim KH
FAU - Kang, Eun Sung
AU  - Kang ES
FAU - Jo, Geoncheol
AU  - Jo G
FAU - Ahn, Se Young
AU  - Ahn SY
FAU - Park, Seon Hwa
AU  - Park SH
FAU - Kim, Sung Il
AU  - Kim SI
AD  - Life Science Division, Amogreentech Co. Ltd. , Seoul 137-902, Korea.
FAU - Mun, Saem
AU  - Mun S
AD  - Life Science Division, Amogreentech Co. Ltd. , Seoul 137-902, Korea.
FAU - Baek, Kyuwon
AU  - Baek K
AD  - Life Science Division, Amogreentech Co. Ltd. , Seoul 137-902, Korea.
FAU - Kim, Byeongyoon
AU  - Kim B
AD  - Department of Chemistry and Research Institute for Natural Sciences, Korea 
      University , Seoul 136-701, Korea.
FAU - Lee, Kwangyeol
AU  - Lee K
AD  - Department of Chemistry and Research Institute for Natural Sciences, Korea 
      University , Seoul 136-701, Korea.
FAU - Yun, Wan Soo
AU  - Yun WS
FAU - Kim, Yong Ho
AU  - Kim YH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151230
PL  - United States
TA  - Bioconjug Chem
JT  - Bioconjugate chemistry
JID - 9010319
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Polysorbates)
SB  - IM
MH  - Antibodies, Bacterial/chemistry
MH  - Bacteriological Techniques/instrumentation/*methods
MH  - Magnetic Resonance Spectroscopy
MH  - Magnetics/instrumentation/methods
MH  - Nanoparticles/*chemistry
MH  - Polysorbates/chemistry
MH  - Salmonella/immunology/*isolation & purification
MH  - Serogroup
MH  - Spectroscopy, Fourier Transform Infrared
EDAT- 2015/12/30 06:00
MHDA- 2017/11/08 06:00
CRDT- 2015/12/30 06:00
PHST- 2015/12/30 06:00 [entrez]
PHST- 2015/12/30 06:00 [pubmed]
PHST- 2017/11/08 06:00 [medline]
AID - 10.1021/acs.bioconjchem.5b00681 [doi]
PST - ppublish
SO  - Bioconjug Chem. 2016 Jan 20;27(1):59-65. doi: 10.1021/acs.bioconjchem.5b00681. 
      Epub 2015 Dec 30.