PMID- 26710889
OWN - NLM
STAT- MEDLINE
DCOM- 20170206
LR  - 20190402
IS  - 1600-0609 (Electronic)
IS  - 0902-4441 (Linking)
VI  - 98
IP  - 1
DP  - 2017 Jan
TI  - The role of ubiquitin-specific protease 14 (USP14) in cell adhesion-mediated drug 
      resistance (CAM-DR) of multiple myeloma cells.
PG  - 4-12
LID - 10.1111/ejh.12729 [doi]
AB  - OBJECTIVE: Cell adhesion-mediated drug resistance (CAM-DR) is one of the 
      mechanisms underlying the drug resistance in multiple myeloma (MM). 
      Ubiquitin-specific protease 14 (USP14) is downregulated in the apoptotic model 
      and upregulated in the adhesive model of MM. This study was undertaken to 
      determine the role of USP14 in CAM-DR of MM cells. METHODS: We examined the 
      expression of USP14 in the apoptotic model of MM. The mechanism of USP14 in the 
      process of apoptosis was further explored by flow cytometry assay and 
      co-immunoprecipitation. We then performed the cell co-culture and adhesion assay 
      and cell viability assay to investigate the effect of USP14 on adhesive rate and 
      drug resistance in MM. RESULTS: We discovered that USP14 played a negative role 
      in cell apoptosis, which is correlated with Bcl-xl. Moreover, overexpression of 
      USP14 in MM cell adhesion model could enhance the ability of cell adhesion by 
      regulating Wnt-signaling pathways, thereby promoting the CAM-DR in MM. 
      CONCLUSION: USP14 participates in CAM-DR of MM through acting as a bridge between 
      Bcl-xl apoptotic pathway and Wnt-signaling pathways and may be represented as a 
      good candidate for pursuing clinical trials in MM.
CI  - (c) 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Xu, Xiaohong
AU  - Xu X
AD  - Department of Pathology, Affiliated Cancer Hospital of Nantong University, 
      Nantong, Jiangsu Province, China.
FAU - Liu, Jing
AU  - Liu J
AD  - Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, 
      Nantong University, Nantong, Jiangsu Province, China.
FAU - Shen, Chaoyan
AU  - Shen C
AD  - Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, 
      Nantong University, Nantong, Jiangsu Province, China.
FAU - Ding, Linlin
AU  - Ding L
AD  - Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, 
      Nantong University, Nantong, Jiangsu Province, China.
FAU - Zhong, Fei
AU  - Zhong F
AD  - Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, 
      Nantong University, Nantong, Jiangsu Province, China.
FAU - Ouyang, Yu
AU  - Ouyang Y
AD  - Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, 
      Nantong University, Nantong, Jiangsu Province, China.
FAU - Wang, Yuchan
AU  - Wang Y
AD  - Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, 
      Nantong University, Nantong, Jiangsu Province, China.
FAU - He, Song
AU  - He S
AD  - Department of Pathology, Affiliated Cancer Hospital of Nantong University, 
      Nantong, Jiangsu Province, China.
LA  - eng
PT  - Journal Article
DEP - 20161102
PL  - England
TA  - Eur J Haematol
JT  - European journal of haematology
JID - 8703985
RN  - 0 (Antineoplastic Agents)
RN  - 0 (USP14 protein, human)
RN  - 0 (bcl-X Protein)
RN  - BZ114NVM5P (Mitoxantrone)
RN  - EC 3.4.19.12 (Ubiquitin Thiolesterase)
SB  - IM
MH  - Antineoplastic Agents/pharmacology
MH  - Apoptosis/drug effects/genetics
MH  - Cell Adhesion/genetics
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects/genetics
MH  - Drug Resistance, Neoplasm/*genetics
MH  - Gene Expression
MH  - Humans
MH  - Mitoxantrone/pharmacology
MH  - Multiple Myeloma/*genetics/metabolism
MH  - Ubiquitin Thiolesterase/*genetics/metabolism
MH  - bcl-X Protein/metabolism
OTO - NOTNLM
OT  - Multiple myeloma
OT  - Ubiquitin-specific protease 14
OT  - apoptosis
OT  - cell adhesion-mediated drug resistance
EDAT- 2016/11/03 06:00
MHDA- 2017/02/07 06:00
CRDT- 2015/12/30 06:00
PHST- 2015/12/21 00:00 [accepted]
PHST- 2016/11/03 06:00 [pubmed]
PHST- 2017/02/07 06:00 [medline]
PHST- 2015/12/30 06:00 [entrez]
AID - 10.1111/ejh.12729 [doi]
PST - ppublish
SO  - Eur J Haematol. 2017 Jan;98(1):4-12. doi: 10.1111/ejh.12729. Epub 2016 Nov 2.