PMID- 26711043
OWN - NLM
STAT- MEDLINE
DCOM- 20160621
LR  - 20181202
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 182
DP  - 2016 Jan 15
TI  - Replication characteristics of eight virulent and two attenuated genotype 1 and 2 
      porcine reproductive and respiratory syndrome virus (PRRSV) strains in nasal 
      mucosa explants.
PG  - 156-62
LID - S0378-1135(15)30085-7 [pii]
LID - 10.1016/j.vetmic.2015.11.016 [doi]
AB  - Porcine reproductive and respiratory syndrome virus (PRRSV) can spread in between 
      pigs via contact and airborne route. It was shown before that the highly 
      pathogenic PRRSV strain Lena was able to replicate 10-100 times more in the nasal 
      mucosa compared to the low pathogenic PRRSV strain LV. In this work, the 
      replication characteristics of four type 1 (LV, 07V063, 08VA, 13V091), three type 
      2 (VR2332, MN-184, VN) and two attenuated (MLV-DV, MLV-VR2332) PRRSV strains were 
      studied. After 72hpi, mean virus titers reached 10(4.5-4.8) TCID50/ml for LV and 
      08VA, 10(5.2-5.4) TCID50/ml for VR2332 and Lena, and 10(5.8-6.3) TCID50/ml for 
      07V063, 13V091, MN-184 and VN strains, whereas attenuated strains remained below 
      detection limit. The mean number of PRRSV-positive cells/mm(2) at 72hpi was 1.1 
      and 1.3 for the attenuated strains and LV, 13.3 for 08VA, 23.5 and 29.3 for 
      VR2332 and 07063, 31.1 and 33.8 for 13V091 and Lena, and, 39.1 and 59.2 for 
      MN-184 and VN respectively. All the LV and MLV-LV infected cells were Sn(+), 
      whereas all other strains also infected Sn(-) macrophages. In conclusion, (i) 
      based on the virus shedding in the respiratory explants, PRRSV strains can be 
      categorized as poor (MLV-DV, MLV-VR2332, LV, 08VA), moderate (Lena, VR2332) and 
      strong (07V063, 13V091, MN-184, VN) secretors, and (ii) based on the number of 
      infected cells isolates can be categorized as low (MLV-DV, MLV-VR2332, LV), 
      moderately (08VA, VR2332), highly (07V063, Lena, 13V091) and hyper (MN-184, VN) 
      virulent in the nasal mucosa.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Frydas, Ilias S
AU  - Frydas IS
AD  - Laboratory of Virology, Department of Virology, Immunology and Parasitology, 
      Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 
      Merelbeke, Belgium. Electronic address: ilias.frydas@ugent.be.
FAU - Nauwynck, Hans J
AU  - Nauwynck HJ
AD  - Laboratory of Virology, Department of Virology, Immunology and Parasitology, 
      Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 
      Merelbeke, Belgium. Electronic address: hans.nauwynck@ugent.be.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151125
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
SB  - IM
MH  - Animals
MH  - DNA Replication
MH  - Genotype
MH  - Nasal Mucosa/*virology
MH  - Porcine Reproductive and Respiratory Syndrome/*genetics
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/isolation & 
      purification/pathogenicity
MH  - Sus scrofa/virology
MH  - Swine
MH  - Virus Shedding
OTO - NOTNLM
OT  - 07V063
OT  - 08VA
OT  - 13V091
OT  - LV
OT  - MLV-DV
OT  - MLV-VR2332
OT  - MN-184
OT  - Nasal mucosa
OT  - PRRSV
OT  - Sialoadhesin
OT  - Type 1
OT  - Type 2
OT  - VN
OT  - VR2332
EDAT- 2015/12/30 06:00
MHDA- 2016/06/22 06:00
CRDT- 2015/12/30 06:00
PHST- 2015/03/24 00:00 [received]
PHST- 2015/11/10 00:00 [revised]
PHST- 2015/11/14 00:00 [accepted]
PHST- 2015/12/30 06:00 [entrez]
PHST- 2015/12/30 06:00 [pubmed]
PHST- 2016/06/22 06:00 [medline]
AID - S0378-1135(15)30085-7 [pii]
AID - 10.1016/j.vetmic.2015.11.016 [doi]
PST - ppublish
SO  - Vet Microbiol. 2016 Jan 15;182:156-62. doi: 10.1016/j.vetmic.2015.11.016. Epub 
      2015 Nov 25.