PMID- 26711233
OWN - NLM
STAT- MEDLINE
DCOM- 20170130
LR  - 20170920
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1021
DP  - 2016 May 15
TI  - Stoichiometric molecularly imprinted polymers for the recognition of anti-cancer 
      pro-drug tegafur.
PG  - 197-203
LID - S1570-0232(15)30340-8 [pii]
LID - 10.1016/j.jchromb.2015.12.015 [doi]
AB  - Molecularly imprinted polymers (MIPs) targeting tegafur, an anti-cancer 
      5-fluorouracil pro-drug, have been prepared by stoichiometric imprinting using 
      2,6-bis(acrylamido)pyridine (BAAPy) as the functional monomer. Solution 
      association between tegafur and BAAPy was studied by (1)H NMR titration, which 
      confirmed the formation of 1:1 complexes with an affinity constant of 574+/-15M(-1) 
      in CDCl3. Evaluation of the synthesised materials by HPLC and equilibrium 
      rebinding experiments revealed high selectivity of the imprinted polymer for the 
      pro-drug vs. 5-fluorouracil and other competing analytes, with maximum imprinting 
      factors of 25.3 and a binding capacity of 45.1mumolg(-1). The synthesised 
      imprinted polymer was employed in solid-phase extraction of the pro-drug using an 
      optimised protocol that included a simple wash with the porogen used in the 
      preparation of the material. Tegafur recoveries of up to 96% were achieved from 
      aqueous samples and 92% from urine samples spiked with the template and three 
      competing analytes. The results demonstrate the potential of the prepared 
      polymers in the pre-concentration of tegafur from biological samples, which could 
      be an invaluable tool in the monitoring of patient compliance and drug uptake and 
      excretion.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Mattos Dos Santos, Paula
AU  - Mattos Dos Santos P
AD  - School of Chemistry and Chemical Engineering, Queen's University Belfast, David 
      Keir Building, Stranmillis Road, BT9 5AG Belfast, Northern Ireland, United 
      Kingdom.
FAU - Hall, Andrew J
AU  - Hall AJ
AD  - Medway School of Pharmacy, Universities of Greenwich and Kent at Medway, Anson 
      Building, Central Avenue, Chatham Maritime, Chatham, Kent, ME4 4TB, United 
      Kingdom.
FAU - Manesiotis, Panagiotis
AU  - Manesiotis P
AD  - School of Chemistry and Chemical Engineering, Queen's University Belfast, David 
      Keir Building, Stranmillis Road, BT9 5AG Belfast, Northern Ireland, United 
      Kingdom. Electronic address: p.manesiotis@qub.ac.uk.
LA  - eng
PT  - Journal Article
DEP - 20151209
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Antineoplastic Agents)
RN  - 1548R74NSZ (Tegafur)
SB  - IM
MH  - Antineoplastic Agents/*analysis/chemistry
MH  - Chromatography, Liquid
MH  - Humans
MH  - Molecular Imprinting/*methods
MH  - Solid Phase Extraction
MH  - Tegafur/*analysis/chemistry
OTO - NOTNLM
OT  - 5-Fluorouracil
OT  - Bioanalysis
OT  - Molecularly imprinted polymers
OT  - Solid-phase extraction
OT  - Tegafur
EDAT- 2015/12/30 06:00
MHDA- 2017/01/31 06:00
CRDT- 2015/12/30 06:00
PHST- 2015/06/09 00:00 [received]
PHST- 2015/12/03 00:00 [revised]
PHST- 2015/12/07 00:00 [accepted]
PHST- 2015/12/30 06:00 [entrez]
PHST- 2015/12/30 06:00 [pubmed]
PHST- 2017/01/31 06:00 [medline]
AID - S1570-0232(15)30340-8 [pii]
AID - 10.1016/j.jchromb.2015.12.015 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2016 May 15;1021:197-203. doi: 
      10.1016/j.jchromb.2015.12.015. Epub 2015 Dec 9.