PMID- 26711324
OWN - NLM
STAT- MEDLINE
DCOM- 20161230
LR  - 20181202
IS  - 2047-2927 (Electronic)
IS  - 2047-2919 (Linking)
VI  - 4
IP  - 1
DP  - 2016 Jan
TI  - Intracavernous delivery of clonal mesenchymal stem cells rescues erectile 
      function in the streptozotocin-induced diabetic mouse.
PG  - 172-84
LID - 10.1111/andr.12138 [doi]
AB  - The major hurdle for the clinical application of stem cell therapy is the 
      heterogeneous nature of the isolated cells, which may cause different treatment 
      outcomes. The aim of this study was to examine the effectiveness of mouse clonal 
      bone marrow-derived stem cells (BMSCs) obtained from a single colony by using 
      subfractionation culturing method for erectile function in diabetic animals. 
      Twelve-week-old C57BL/6J mice were divided into four groups: controls, diabetic 
      mice, and diabetic mice treated with a single intracavernous injection of PBS (20 
      muL) or clonal BMSCs (3 x 10(5) cells/20 muL). Clonal BMSCs were isolated from 
      5-week-old C3H mice. Two weeks after treatment, erectile function was measured by 
      electrical stimulation of the cavernous nerve. The penis was stained with 
      antibodies to PECAM-1, smooth muscle alpha-actin, neuronal nitric oxide synthase 
      (nNOS), neurofilament, and phosphorylated endothelial NOS (phospho-eNOS). We also 
      performed Western blot for phospho-eNOS, and eNOS in the corpus cavernosum 
      tissue. Local delivery of clonal BMSCs significantly restored cavernous 
      endothelial and smooth muscle cell contents, and penile nNOS and neurofilament 
      contents, and induced eNOS phosphorylation (Ser1177) in diabetic mice. 
      Intracavernous injection of clonal BMSCs induced significant recovery of erectile 
      function, which reached 80-90% of the control values. Clonal BMSCs successfully 
      restored erectile function through dual angiogenic and neurotrophic effects in 
      diabetic mice. The homogenous nature of clonal mesenchymal stem cells may allow 
      their clinical applications and open a new avenue through which to treat diabetic 
      erectile dysfunction.
CI  - (c) 2015 American Society of Andrology and European Academy of Andrology.
FAU - Ryu, J-K
AU  - Ryu JK
AD  - National Research Center for Sexual Medicine and Department of Urology, Inha 
      University School of Medicine, Incheon, Korea.
AD  - Inha Research Institute for Medical Sciences, Inha University School of Medicine, 
      Incheon, Korea.
FAU - Kim, D-H
AU  - Kim DH
AD  - Translational Research Center, Inha University School of Medicine, Incheon, 
      Korea.
FAU - Song, K-M
AU  - Song KM
AD  - National Research Center for Sexual Medicine and Department of Urology, Inha 
      University School of Medicine, Incheon, Korea.
FAU - Ryu, D-S
AU  - Ryu DS
AD  - Department of Urology, Sungkyunkwan University School of Medicine, Samsung 
      Changwon Hospital, Changwon, Korea.
FAU - Kim, S-N
AU  - Kim SN
AD  - Drug Development Program, Department of Medicine, Inha University School of 
      Medicine, Incheon, Korea.
FAU - Shin, D-H
AU  - Shin DH
AD  - SCM Lifescience Co., Ltd., Incheon, Korea.
FAU - Yi, T
AU  - Yi T
AD  - Translational Research Center, Inha University School of Medicine, Incheon, 
      Korea.
FAU - Suh, J-K
AU  - Suh JK
AD  - National Research Center for Sexual Medicine and Department of Urology, Inha 
      University School of Medicine, Incheon, Korea.
FAU - Song, S U
AU  - Song SU
AD  - Translational Research Center, Inha University School of Medicine, Incheon, 
      Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151229
PL  - England
TA  - Andrology
JT  - Andrology
JID - 101585129
RN  - 0 (Actins)
RN  - 0 (Platelet Endothelial Cell Adhesion Molecule-1)
RN  - 0 (alpha-smooth muscle actin, mouse)
RN  - 5W494URQ81 (Streptozocin)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type I)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
RN  - EC 1.14.13.39 (Nos3 protein, mouse)
SB  - IM
MH  - Actins/analysis
MH  - Animals
MH  - Cell- and Tissue-Based Therapy/*methods
MH  - Diabetes Complications/*therapy
MH  - Diabetes Mellitus, Experimental/metabolism
MH  - Disease Models, Animal
MH  - Erectile Dysfunction/*therapy
MH  - Intermediate Filaments/metabolism
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/cytology
MH  - Mice, Inbred C3H
MH  - Mice, Inbred C57BL
MH  - Nitric Oxide Synthase Type I/analysis
MH  - Nitric Oxide Synthase Type III/analysis
MH  - Penile Erection/*physiology
MH  - Phosphorylation
MH  - Platelet Endothelial Cell Adhesion Molecule-1/analysis
MH  - Recovery of Function
MH  - Streptozocin
OTO - NOTNLM
OT  - angiogenesis
OT  - clonal mesenchymal stem cell
OT  - diabetes
OT  - penile erection
OT  - stem cell therapy
EDAT- 2015/12/30 06:00
MHDA- 2016/12/31 06:00
CRDT- 2015/12/30 06:00
PHST- 2015/04/02 00:00 [received]
PHST- 2015/09/02 00:00 [revised]
PHST- 2015/10/26 00:00 [accepted]
PHST- 2015/12/30 06:00 [entrez]
PHST- 2015/12/30 06:00 [pubmed]
PHST- 2016/12/31 06:00 [medline]
AID - 10.1111/andr.12138 [doi]
PST - ppublish
SO  - Andrology. 2016 Jan;4(1):172-84. doi: 10.1111/andr.12138. Epub 2015 Dec 29.