PMID- 26711615
OWN - NLM
STAT- MEDLINE
DCOM- 20160330
LR  - 20211203
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 95
IP  - 32
DP  - 2015 Aug 25
TI  - [Study of immunomodulatory function of exosomes derived from human umbilical cord 
      mesenchymal stem cells].
PG  - 2630-3
AB  - OBJECTIVE: To investigate the immumodulation ability of exosomes secreted from 
      human umbilical cord-derived mesenchymal stem cells (hUC-MSCs). METHODS: hUC-MSCs 
      were isolated and cultured.Exosomes were isolated from the culture media of the 
      third-generation hUC-MSCs. The expression of specific surface marker CD9 and CD81 
      were detected by Western blot, and the concentration of hUC-MSCs 
      exosomes(hUC-MSCs-ex) was evaluated by BCA assay. CD3/CD28-stimulated peripheral 
      blood mononuclear cells(PBMCs) from healthy donor were co-cultured with different 
      concentration of hUC-MSCs-ex for 72 h. The percentage of Th17 and Treg cells and 
      the proliferation of CD4(+) and CD8(+) T cells were detected by flow cytometry. ELISA 
      was used to test the level of IFN-gamma, IL-6, TNF-alpha and TGF-beta1. RESULTS: hUC-MSCs-ex 
      inhibited the proliferation of CD4(+) and CD8(+) cells obviously,and increased the 
      proportion of CD4(+) CD25(+) FoxP3(+) Treg cells, with high expression of CD81 and CD9. 
      After CD3/CD28 monoclonal antibody stimulated, the percentage of CD45(+) CD4(+) Ki67(+) 
      and CD45(+) CD8(+) Ki67(+) cells were 85.3% +/- 5.6% and 72.6% +/- 6.3%, respectively. 
      Meanwhile, the level of TGF-beta1 were elevated and the level of IFN-gamma, IL-6 and 
      TNF-alpha were decreased (P<0.05). CONCLUSION: hUC-MSCs-ex has the immunomodulatory 
      functionin vitro, which could be a new therapeutic agent for the treatment of 
      immune disorders.
FAU - Liu, Ming
AU  - Liu M
AD  - State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory 
      Diseases, the First Affiliated Hospital of Guangzhou Medical University, 
      Guangzhou Medical University, Guangzhou 510120, China.
FAU - Wang, Jinsong
AU  - Wang J
FAU - Liu, Muyun
AU  - Liu M
FAU - Hu, Xiang
AU  - Hu X
AD  - Shenzhen Beike Cell Engineering Research Institute, Shenzhen 518057, China, 
      Email: huxiang@beike.cc.
FAU - Xu, Jun
AU  - Xu J
AD  - Email: xufeili@vip.163.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-6)
RN  - 0 (TNF protein, human)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - CD8-Positive T-Lymphocytes
MH  - Coculture Techniques
MH  - *Exosomes
MH  - Flow Cytometry
MH  - Humans
MH  - Immunosuppression Therapy
MH  - Interleukin-6
MH  - Leukocytes, Mononuclear
MH  - *Mesenchymal Stem Cells
MH  - T-Lymphocytes, Regulatory
MH  - Th17 Cells
MH  - Transforming Growth Factor beta1
MH  - Tumor Necrosis Factor-alpha
MH  - *Umbilical Cord
EDAT- 2015/12/30 06:00
MHDA- 2016/03/31 06:00
CRDT- 2015/12/30 06:00
PHST- 2015/12/30 06:00 [entrez]
PHST- 2015/12/30 06:00 [pubmed]
PHST- 2016/03/31 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2015 Aug 25;95(32):2630-3.