PMID- 26711973
OWN - NLM
STAT- MEDLINE
DCOM- 20160330
LR  - 20181202
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 95
IP  - 34
DP  - 2015 Sep 8
TI  - [Research of the efficiency of different lupus anticoagulant assays].
PG  - 2760-5
AB  - OBJECTIVE: To provide valuable test by evaluating the efficiency of lupus 
      anticoagulant assays based on different principles. METHODS: 370 citrated samples 
      were recruited, LA were tested by dRVVT, SCT, APTT-Actin, APTT-Actin FSL and 
      APTT-SS respectively, and 1:1 mixing tests before incubation were studied in all 
      the recruited subjects. For LA testing, the results of 1:1 mixing test were 
      expressed as index of circulating anticoagulant (ICA), and the confirm test 
      results were expressed as percent of correction (C) and normalized ratio (NR). 
      The reference interval (RI)/Cut-off values of all the assays were established, 
      and the efficiency of all the assays were further studied. RESULTS: The 
      RI/Cut-off values of all assays were different. The sensitivity of dRVVT were the 
      highest among all the assays (70.3%-93.1%), and the sensitivity of APTT (<55.0%) 
      were the lowest. The sensitivity of APTT-SS (47.5%-53.5%) were higher than 
      APTT-Actin (32.7%-39.6%) and APTT-Actin FSL (29.7%-36.6%). The specificity of 
      dRVVT, SCT and three APTT were 91%-100%, 80.6%-100%, and 85.1%-98.5%, 
      respectively. The specificity of SCT confirm test were 100%. Sensitivity of all 
      the five methods were decreased in the weak positive LA. The sensitivity of dRVVT 
      and SCT were more than 50.0%, however, the sensitivity of APTT-Actin and 
      APTT-Acitn FSL were less than 16.0%. The sensitivity of APTT-SS were 23.7%-30.5%. 
      Except the APTT-SS, dRVVT and SCT were correlated with thrombotic risk in APS 
      patients (OR: 5.562-9.240, P<0.05). CONCLUSIONS: Every Laboratory should 
      establish local LA RI/Cut-off values. dRVVT is the best assay for LA test, and 
      APTT-SS method is superior to the other APTT method. The different test 
      combinations of dRVVT and SCT are correlated with thrombotic risk in APS 
      patients.
FAU - Shou, Weiling
AU  - Shou W
AD  - Department of Laboratory Medicine, Peking Union Medical College Hospital, Chinese 
      Academic Medical Science and Peking Union Medical College, Beijing 100730, China.
FAU - Chen, Qian
AU  - Chen Q
AD  - Department of Laboratory Medicine, Peking Union Medical College Hospital, Chinese 
      Academic Medical Science and Peking Union Medical College, Beijing 100730, China.
FAU - Wu, Wei
AU  - Wu W
AD  - Department of Laboratory Medicine, Peking Union Medical College Hospital, Chinese 
      Academic Medical Science and Peking Union Medical College, Beijing 100730, China.
FAU - Fan, Liankai
AU  - Fan L
AD  - Department of Laboratory Medicine, Peking Union Medical College Hospital, Chinese 
      Academic Medical Science and Peking Union Medical College, Beijing 100730, China.
FAU - Xie, Tian
AU  - Xie T
AD  - Department of Laboratory Medicine, Peking Union Medical College Hospital, Chinese 
      Academic Medical Science and Peking Union Medical College, Beijing 100730, China.
FAU - Cui, Wei
AU  - Cui W
AD  - Department of Laboratory Medicine, Peking Union Medical College Hospital, Chinese 
      Academic Medical Science and Peking Union Medical College, Beijing 100730, China; 
      Email: cuiw@pumch.cn.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Anticoagulants)
RN  - 0 (Lupus Coagulation Inhibitor)
SB  - IM
MH  - Anticoagulants
MH  - Humans
MH  - *Lupus Coagulation Inhibitor
MH  - Reference Values
MH  - Thrombosis
EDAT- 2015/12/30 06:00
MHDA- 2016/03/31 06:00
CRDT- 2015/12/30 06:00
PHST- 2015/12/30 06:00 [entrez]
PHST- 2015/12/30 06:00 [pubmed]
PHST- 2016/03/31 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2015 Sep 8;95(34):2760-5.