PMID- 2671215
OWN - NLM
STAT- MEDLINE
DCOM- 19891003
LR  - 20071114
IS  - 0022-2143 (Print)
IS  - 0022-2143 (Linking)
VI  - 114
IP  - 3
DP  - 1989 Sep
TI  - The effects of age on isotypic antibody responses to Aspergillus fumigatus: 
      implications regarding in vitro measurements.
PG  - 278-84
AB  - The demonstration of elevated serum immunoglobulin E (IgE), immunoglobulin G 
      (IgG), and immunoglobulin A (IgA) antibodies to Aspergillus fumigatus (Af) is a 
      valuable aid in the serodiagnosis of allergic bronchopulmonary aspergillosis 
      (ABPA). It is important to recognize cases of ABPA, because repeated untreated 
      episodes of pulmonary infiltrates can result in bronchiectasis and even fatal 
      fibrotic lung disease. Serum samples from patients with ABPA have increased 
      isotypic antibodies to Af when compared with patients with asthma and immediate 
      cutaneous reactivity but in whom ABPA has been excluded. There is little 
      information regarding age-related changes of isotypic antibodies to a specific 
      allergen or in comparison of such changes with age-related changes in total 
      class-specific antibody. We determined IgE-Af, IgG-Af, and IgA-Af and total IgE, 
      IgG, and IgA concentrations in serum samples from children with asthma (ages less 
      than 1 through 5 years, 6 through 10 years, and 11 through 15 years) and compared 
      the results with those in children with APBA and adults with asthma. The use of 
      an indirect amplified ELISA with Af adsorbed to microtiter wells demonstrated 
      that serum samples from children with ABPA have elevated IgE-Af, IgG-Af, and 
      IgA-Af concentrations compared with those in serum samples from children with 
      asthma. The assays were sensitive and highly specific in discriminating ABPA from 
      asthma in children. Isotypic antibodies to Af in children do not demonstrate 
      age-related increases as occur for total serum IgE, IgG, and IgA 
      concentrations.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Greenberger, P A
AU  - Greenberger PA
AD  - Department of Medicine, Northwestern University Medical School, Chicago, IL 
      60611.
FAU - Liotta, J L
AU  - Liotta JL
FAU - Roberts, M
AU  - Roberts M
LA  - eng
GR  - AI 11403/AI/NIAID NIH HHS/United States
GR  - AI 23071/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Lab Clin Med
JT  - The Journal of laboratory and clinical medicine
JID - 0375375
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Aging/*immunology
MH  - *Antibody Formation
MH  - Aspergillus fumigatus/*immunology
MH  - Asthma/*immunology
MH  - Child
MH  - Child, Preschool
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - *Hypersensitivity, Immediate
MH  - Immunoglobulin A/analysis
MH  - Immunoglobulin E/analysis
MH  - Immunoglobulin G/analysis
MH  - Infant
MH  - Precipitin Tests
MH  - Reference Values
EDAT- 1989/09/01 00:00
MHDA- 1989/09/01 00:01
CRDT- 1989/09/01 00:00
PHST- 1989/09/01 00:00 [pubmed]
PHST- 1989/09/01 00:01 [medline]
PHST- 1989/09/01 00:00 [entrez]
PST - ppublish
SO  - J Lab Clin Med. 1989 Sep;114(3):278-84.