PMID- 26712211
OWN - NLM
STAT- MEDLINE
DCOM- 20170227
LR  - 20181113
IS  - 1993-0402 (Electronic)
IS  - 1672-0415 (Linking)
VI  - 22
IP  - 12
DP  - 2016 Dec
TI  - Panax notoginseng saponins protect kidney from diabetes by up-regulating silent 
      information regulator 1 and activating antioxidant proteins in rats.
PG  - 910-917
AB  - OBJECTIVE: To explore the mechanism of the protective effects of Panax 
      notoginseng saponins (PNS) on kidney in diabetic rats. METHODS: Diabetic rat 
      model was obtained by intravenous injection of alloxan, and the rats were divided 
      into model, PNS-100 mg/(kg day) and PNS-200 mg/(kg day) groups, 10 each. Another 
      10 rats injected with saline were served as control. Periodic acid-Schiff 
      staining and immunological histological chemistry were used to observe 
      histomorphology and tissue expression of bone morphogenetic protein-7 (BMP-7). 
      Silent information regulator 1 (SIRT1) was silenced in rat mesangial cells by RNA 
      interference. The mRNA expressions of SIRT-1, monocyte chemoattractant protein-1 
      (MCP-1), transforming growth factor beta1 (TGF-beta1) and plasminogen activator 
      inhibitor-1 (PAI-1) were analyzed by reverse transcription polymerase chain 
      reaction. The protein expressions of SIRT1 and the acetylation of nuclear factor 
      kappaB (NF-kappaB) P65 were determined by western blotting. The concentration of MCP-1, 
      TGF-beta1 and malondialdehyde (MDA) in culture supernatant were detected by 
      enzyme-linked immuno sorbent assay. The activity of superoxide dismutase (SOD) 
      was detected by the classical method of nitrogen and blue four. RESULTS: In 
      diabetic model rats, PNS could not only reduce blood glucose and lipid (P<0.01), 
      but also increase protein level of BMP-7 and inhibit PAI-1 expression for 
      suppressing fibrosis of the kidney. In rat mesangial cells, PNS could up-regulate 
      the expression of SIRT1 (P<0.01) and in turn suppress the transcription of TGF-beta1 
      (P<0.05) and MCP-1 (P<0.05). PNS could also reverse the increased acetylation of 
      NF-kappaB p65 by high glucose. In addition, redox regulation factor MDA was 
      down-regulated (P<0.05) and SOD was up-regulated (P<0.01), which were both 
      induced by SIRT1 up-regulation. CONCLUSIONS: PNS could protect kidney from 
      diabetes with the possible mechanism of up-regulating SIRT1, therefore inhibiting 
      inflammation through decreasing the induction of inflammatory cytokines and 
      TGF-beta1, as well as activating antioxidant proteins.
FAU - Du, Yue-Guang
AU  - Du YG
AD  - Department of Pathyology, Zhejiang Chinese Medical University, Hangzhou, 310053, 
      China.
FAU - Wang, Li-Pei
AU  - Wang LP
AD  - Department of Pathyology, Zhejiang Chinese Medical University, Hangzhou, 310053, 
      China.
FAU - Qian, Jun-Wen
AU  - Qian JW
AD  - National Clinical Research Center of Traditional Chinese Medicine, Zhejiang 
      Chinese Medical University, Hangzhou, 310053, China.
FAU - Zhang, Ke-Na
AU  - Zhang KN
AD  - Department of Pathyology, Zhejiang Chinese Medical University, Hangzhou, 310053, 
      China.
FAU - Chai, Ke-Fu
AU  - Chai KF
AD  - National Clinical Research Center of Traditional Chinese Medicine, Zhejiang 
      Chinese Medical University, Hangzhou, 310053, China. ckf666@163.com.
LA  - eng
PT  - Journal Article
DEP - 20151228
PL  - China
TA  - Chin J Integr Med
JT  - Chinese journal of integrative medicine
JID - 101181180
RN  - 0 (Antioxidants)
RN  - 0 (Blood Glucose)
RN  - 0 (Bone Morphogenetic Protein 7)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipids)
RN  - 0 (Plasminogen Activator Inhibitor 1)
RN  - 0 (Protective Agents)
RN  - 0 (Saponins)
RN  - 0 (Transcription Factor RelA)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 3.5.1.- (Sirtuin 1)
SB  - IM
MH  - Acetylation/drug effects
MH  - Animals
MH  - Antioxidants/*metabolism
MH  - Blood Glucose/metabolism
MH  - Bone Morphogenetic Protein 7/metabolism
MH  - Chemokine CCL2/metabolism
MH  - Diabetes Mellitus, Experimental/blood/*drug therapy/genetics/physiopathology
MH  - Gene Knockdown Techniques
MH  - Immunohistochemistry
MH  - Kidney/drug effects/*pathology
MH  - Kidney Function Tests
MH  - Lipids/blood
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Mesangial Cells/drug effects/metabolism
MH  - Oxidative Stress/drug effects
MH  - Panax notoginseng/*chemistry
MH  - Plasminogen Activator Inhibitor 1/genetics/metabolism
MH  - Protective Agents/pharmacology/*therapeutic use
MH  - Rats, Sprague-Dawley
MH  - Saponins/pharmacology/*therapeutic use
MH  - Sirtuin 1/*genetics
MH  - Superoxide Dismutase/metabolism
MH  - Transcription Factor RelA/metabolism
MH  - Transcription, Genetic/drug effects
MH  - Transforming Growth Factor beta1/metabolism
MH  - Up-Regulation/*drug effects
OTO - NOTNLM
OT  - Chinese medicine
OT  - Panax notoginseng saponins
OT  - diabetic nephropathy
OT  - inflammation
OT  - nuclear factor kappaB
OT  - silent information regulator 1
EDAT- 2015/12/30 06:00
MHDA- 2017/02/28 06:00
CRDT- 2015/12/30 06:00
PHST- 2015/03/04 00:00 [received]
PHST- 2015/12/30 06:00 [pubmed]
PHST- 2017/02/28 06:00 [medline]
PHST- 2015/12/30 06:00 [entrez]
AID - 10.1007/s11655-015-2446-1 [pii]
AID - 10.1007/s11655-015-2446-1 [doi]
PST - ppublish
SO  - Chin J Integr Med. 2016 Dec;22(12):910-917. doi: 10.1007/s11655-015-2446-1. Epub 
      2015 Dec 28.