PMID- 26712731
OWN - NLM
STAT- MEDLINE
DCOM- 20161006
LR  - 20201209
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 21
IP  - 1
DP  - 2015 Dec 25
TI  - A Herbal Formula, Atofreellage, Ameliorates Atopic Dermatitis-Like Skin Lesions 
      in an NC/Nga Mouse Model.
PG  - E35
LID - 10.3390/molecules21010035 [doi]
LID - 35
AB  - We evaluated the anti-atopic dermatitis (AD) effect of Atofreellage (AF), a 
      herbal formula composed of 10 medicinal plants. AD was induced on the dorsal skin 
      areas of NC/Nga mice (male, seven weeks old) by daily application of 
      2,4-dinitrochlorobenzene (DNCB) for five weeks. After three weeks of DNCB 
      application, 200 muL of AF (0, 25, 50 or 100 mg/mL) was applied to the skin 
      lesions. Histological findings, blood cell populations, serum levels of 
      immunoglobulin E (IgE), histamine, pro-inflammatory cytokines, and inflammatory 
      signaling in the skin tissue, and T-helper cell type 2 (Th(2))-related cytokines in 
      splenocytes were analyzed. Histopathological findings showed AF treatment notably 
      attenuated the thickness of dorsal skin, and eosinophil infiltration. AF 
      treatment (especially 100 mg/mL) also demonstrably ameliorated the blood cell 
      population abnormalities, as the notable elevation of serum concentrations of 
      IgE, histamine, TNF-alpha, IL-6 and IL-1beta were remarkably normalized by AF treatment. 
      Western blot analysis evidenced the apparent normalization of inflammatory 
      signals (ERK, p38 MAP kinase, JNK, and NF-kappaB) in the skin tissue. Additionally, 
      AF treatment notably attenuated the activation of Th(2)-dominant cytokines (IL-13, 
      IL-4, and IL-5) in Con A-treated splenocytes in an ex vivo assay. In conclusion, 
      this study provides experimental evidence for the clinical relevance of 
      Atofreellage.
FAU - Kim, Won-Yong
AU  - Kim WY
AD  - Liver and Immunology Research-Center, Daejeon Oriental Hospital of Daejeon 
      University, 176-9, Daeheung-ro, Jung-Gu, Daejeon 34929, Korea. 
      godunga3@naver.com.
FAU - Kim, Hyeong-Geug
AU  - Kim HG
AD  - Liver and Immunology Research-Center, Daejeon Oriental Hospital of Daejeon 
      University, 176-9, Daeheung-ro, Jung-Gu, Daejeon 34929, Korea. winakim@dju.kr.
FAU - Lee, Hye-Won
AU  - Lee HW
AD  - TKM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine, 
      Daejeon 34054, Korea. hwlee@kiom.re.kr.
FAU - Lee, Jin-Seok
AU  - Lee JS
AD  - Liver and Immunology Research-Center, Daejeon Oriental Hospital of Daejeon 
      University, 176-9, Daeheung-ro, Jung-Gu, Daejeon 34929, Korea. 
      neptune26ljs@naver.com.
FAU - Im, Hwi-Jin
AU  - Im HJ
AD  - Liver and Immunology Research-Center, Daejeon Oriental Hospital of Daejeon 
      University, 176-9, Daeheung-ro, Jung-Gu, Daejeon 34929, Korea. 
      lastdohee@gmail.com.
FAU - Kim, Hyo-Seon
AU  - Kim HS
AD  - Liver and Immunology Research-Center, Daejeon Oriental Hospital of Daejeon 
      University, 176-9, Daeheung-ro, Jung-Gu, Daejeon 34929, Korea. 
      khs910707@hanmail.net.
FAU - Lee, Sung-Bae
AU  - Lee SB
AD  - Liver and Immunology Research-Center, Daejeon Oriental Hospital of Daejeon 
      University, 176-9, Daeheung-ro, Jung-Gu, Daejeon 34929, Korea. 
      sky161300@naver.com.
FAU - Son, Chang-Gue
AU  - Son CG
AD  - Liver and Immunology Research-Center, Daejeon Oriental Hospital of Daejeon 
      University, 176-9, Daeheung-ro, Jung-Gu, Daejeon 34929, Korea. ckson@dju.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151225
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Cytokines)
RN  - 0 (Dinitrochlorobenzene)
RN  - 0 (Plant Extracts)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 820484N8I3 (Histamine)
SB  - IM
MH  - Animals
MH  - Cytokines/metabolism
MH  - Dermatitis, Atopic/chemically induced/*drug therapy/*immunology
MH  - Dinitrochlorobenzene/adverse effects
MH  - Disease Models, Animal
MH  - Histamine/metabolism
MH  - Immunoglobulin E/metabolism
MH  - MAP Kinase Signaling System/drug effects
MH  - Male
MH  - Mice
MH  - Plant Extracts/*administration & dosage/chemistry/pharmacology
MH  - Spleen/*drug effects/immunology
MH  - Th2 Cells/metabolism
PMC - PMC6273903
OTO - NOTNLM
OT  - NC/Nga murine model
OT  - atopic dermatitis
OT  - herbal medicine
OT  - inflammation
COIS- The authors declare no conflict of interest.
EDAT- 2015/12/30 06:00
MHDA- 2016/10/08 06:00
PMCR- 2015/12/25
CRDT- 2015/12/30 06:00
PHST- 2015/11/07 00:00 [received]
PHST- 2015/12/18 00:00 [revised]
PHST- 2015/12/21 00:00 [accepted]
PHST- 2015/12/30 06:00 [entrez]
PHST- 2015/12/30 06:00 [pubmed]
PHST- 2016/10/08 06:00 [medline]
PHST- 2015/12/25 00:00 [pmc-release]
AID - molecules21010035 [pii]
AID - molecules-21-00035 [pii]
AID - 10.3390/molecules21010035 [doi]
PST - epublish
SO  - Molecules. 2015 Dec 25;21(1):E35. doi: 10.3390/molecules21010035.