PMID- 26714275
OWN - NLM
STAT- MEDLINE
DCOM- 20160706
LR  - 20201215
IS  - 1553-7404 (Electronic)
IS  - 1553-7390 (Print)
IS  - 1553-7390 (Linking)
VI  - 11
IP  - 12
DP  - 2015 Dec
TI  - Arabidopsis COP1 SUPPRESSOR 2 Represses COP1 E3 Ubiquitin Ligase Activity through 
      Their Coiled-Coil Domains Association.
PG  - e1005747
LID - 10.1371/journal.pgen.1005747 [doi]
LID - e1005747
AB  - CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) functions as an E3 ubiquitin ligase and 
      mediates a variety of developmental processes in Arabidopsis by targeting a 
      number of key regulators for ubiquitination and degradation. Here, we identify a 
      novel COP1 interacting protein, COP1 SUPPRESSOR 2 (CSU2). Loss of function 
      mutations in CSU2 suppress the constitutive photomorphogenic phenotype of cop1-6 
      in darkness. CSU2 directly interacts with COP1 via their coiled-coil domains and 
      is recruited by COP1 into nuclear speckles in living plant cells. Furthermore, 
      CSU2 inhibits COP1 E3 ubiquitin ligase activity in vitro, and represses COP1 
      mediated turnover of HY5 in cell-free extracts. We propose that in csu2 cop1-6 
      mutants, the lack of CSU2's repression of COP1 allows the low level of COP1 to 
      exhibit higher activity that is sufficient to prevent accumulation of HY5 in the 
      dark, thus restoring the etiolated phenotype. In addition, CSU2 is required for 
      primary root development under normal light growth condition.
FAU - Xu, Dongqing
AU  - Xu D
AD  - State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center 
      for Life Sciences, School of Advanced Agriculture Sciences and School of Life 
      Sciences, Peking University, Beijing, China.
AD  - Department of Biological and Environmental Sciences, Gothenburg University, 
      Gothenburg, Sweden.
FAU - Lin, Fang
AU  - Lin F
AD  - State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center 
      for Life Sciences, School of Advanced Agriculture Sciences and School of Life 
      Sciences, Peking University, Beijing, China.
FAU - Jiang, Yan
AU  - Jiang Y
AD  - State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center 
      for Life Sciences, School of Advanced Agriculture Sciences and School of Life 
      Sciences, Peking University, Beijing, China.
AD  - Department of Biological and Environmental Sciences, Gothenburg University, 
      Gothenburg, Sweden.
FAU - Ling, Junjie
AU  - Ling J
AD  - State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center 
      for Life Sciences, School of Advanced Agriculture Sciences and School of Life 
      Sciences, Peking University, Beijing, China.
FAU - Hettiarachchi, Chamari
AU  - Hettiarachchi C
AD  - Department of Chemistry, University of Colombo, Colombo, Sri Lanka.
FAU - Tellgren-Roth, Christian
AU  - Tellgren-Roth C
AD  - Uppsala Genome Center, National Genomics Infrastructure, Science for Life 
      Laboratory, Department of Immunology, Genetics and Pathology, Uppsala University, 
      BMC, Uppsala, Sweden.
FAU - Holm, Magnus
AU  - Holm M
AD  - Department of Biological and Environmental Sciences, Gothenburg University, 
      Gothenburg, Sweden.
FAU - Wei, Ning
AU  - Wei N
AD  - Department of Molecular, Cellular, and Developmental Biology, Yale University, 
      New Haven, Connecticut, United States of America.
FAU - Deng, Xing Wang
AU  - Deng XW
AD  - State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center 
      for Life Sciences, School of Advanced Agriculture Sciences and School of Life 
      Sciences, Peking University, Beijing, China.
LA  - eng
GR  - R01 GM047850/GM/NIGMS NIH HHS/United States
GR  - R37 GM047850/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151229
PL  - United States
TA  - PLoS Genet
JT  - PLoS genetics
JID - 101239074
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Basic-Leucine Zipper Transcription Factors)
RN  - 0 (CSU2 protein, Arabidopsis)
RN  - 0 (HY5 protein, Arabidopsis)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Repressor Proteins)
RN  - EC 2.3.2.27 (AT2G32950 protein, Arabidopsis)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Arabidopsis/genetics/*metabolism
MH  - Arabidopsis Proteins/*genetics/*metabolism
MH  - Basic-Leucine Zipper Transcription Factors/metabolism
MH  - Binding Sites
MH  - Mutation
MH  - Nuclear Proteins/metabolism
MH  - Protein Binding
MH  - Repressor Proteins/*genetics/metabolism
MH  - Ubiquitin-Protein Ligases/genetics/*metabolism
PMC - PMC4694719
COIS- The authors have declared that no competing interests exist.
EDAT- 2015/12/30 06:00
MHDA- 2016/07/07 06:00
PMCR- 2015/12/29
CRDT- 2015/12/30 06:00
PHST- 2015/07/25 00:00 [received]
PHST- 2015/11/27 00:00 [accepted]
PHST- 2015/12/30 06:00 [entrez]
PHST- 2015/12/30 06:00 [pubmed]
PHST- 2016/07/07 06:00 [medline]
PHST- 2015/12/29 00:00 [pmc-release]
AID - PGENETICS-D-15-01851 [pii]
AID - 10.1371/journal.pgen.1005747 [doi]
PST - epublish
SO  - PLoS Genet. 2015 Dec 29;11(12):e1005747. doi: 10.1371/journal.pgen.1005747. 
      eCollection 2015 Dec.