PMID- 26715273
OWN - NLM
STAT- MEDLINE
DCOM- 20170216
LR  - 20170216
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 37
IP  - 6
DP  - 2016 Jun
TI  - CUL4A expression in pediatric osteosarcoma tissues and its effect on cell growth 
      in osteosarcoma cells.
PG  - 8139-44
LID - 10.1007/s13277-015-4715-1 [doi]
AB  - Osteosarcoma (OS) is the most common bone malignancy in the pediatric population, 
      and it comprises about 3 % of all pediatric tumors. Aberrant expression of the 
      Cullin 4A (CUL4A) is found in many tumor types, but the role of CUL4A in OS 
      progression remains largely unknown. The aim of this study was to investigate the 
      expression and function of CUL4A in OS. CUL4A expression was detected in 30 
      samples of surgically resected OS and matched tumor-adjacent tissues using 
      quantitative reverse transcription polymerase chain reaction (qRT-PCR) and 
      Western blot. Cell proliferation was assessed by MTT, and migration and invasion 
      were analyzed by Transwell and Matrigel assays after CUL4A knockdown in OS in 
      vitro. Our result showed increased CUL4A expression in OS tissues. CUL4A 
      knockdown inhibited the proliferation of MG63 cells. Furthermore, CUL4A siRNA 
      ameliorated the migration and invasion of MG63 cell lines with altered expression 
      of epithelial-mesenchymal transition (EMT)-associated molecules. Taken together, 
      our findings indicate that CUL4A plays a pivotal role in OS progression and may 
      serve as a potential marker for clinical diagnosis and target for therapy.
FAU - Li, Xin
AU  - Li X
AD  - Department of Pediatric Surgery, General Hospital of PLA, FuXing Road 28#, 
      Haidian District, Beijing, 100853, China.
AD  - Department of Pediatric Orthopaedic, Hunan Provincial People's Hospital, 
      Changsha, 410005, China.
FAU - Xu, Ruijiang
AU  - Xu R
AD  - Department of Pediatric Surgery, General Hospital of PLA, FuXing Road 28#, 
      Haidian District, Beijing, 100853, China. plaruijiangxu@126.com.
FAU - Liu, Hong
AU  - Liu H
AD  - Department of Pediatric Orthopaedic, Hunan Provincial People's Hospital, 
      Changsha, 410005, China.
FAU - Fang, Ke
AU  - Fang K
AD  - Department of Pediatric Orthopaedic, Hunan Provincial People's Hospital, 
      Changsha, 410005, China.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20151229
PL  - Netherlands
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental 
      Biology and Medicine
JID - 8409922
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (CUL4A protein, human)
RN  - 0 (Cullin Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Apoptosis
MH  - Biomarkers, Tumor/genetics/*metabolism
MH  - Blotting, Western
MH  - Bone Neoplasms/genetics/metabolism/*pathology
MH  - Case-Control Studies
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Child
MH  - Child, Preschool
MH  - Cullin Proteins/antagonists & inhibitors/genetics/*metabolism
MH  - Epithelial-Mesenchymal Transition
MH  - Follow-Up Studies
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Infant
MH  - Infant, Newborn
MH  - Neoplasm Invasiveness
MH  - Neoplasm Staging
MH  - Osteosarcoma/genetics/metabolism/*pathology
MH  - Prognosis
MH  - RNA, Messenger/genetics
MH  - RNA, Small Interfering/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction
MH  - Tumor Cells, Cultured
MH  - Young Adult
OTO - NOTNLM
OT  - CUL4A
OT  - EMT
OT  - Invasion
OT  - Osteosarcoma
OT  - Proliferation
OT  - Targeted therapy
EDAT- 2015/12/31 06:00
MHDA- 2017/02/17 06:00
CRDT- 2015/12/31 06:00
PHST- 2015/11/30 00:00 [received]
PHST- 2015/12/20 00:00 [accepted]
PHST- 2015/12/31 06:00 [entrez]
PHST- 2015/12/31 06:00 [pubmed]
PHST- 2017/02/17 06:00 [medline]
AID - 10.1007/s13277-015-4715-1 [pii]
AID - 10.1007/s13277-015-4715-1 [doi]
PST - ppublish
SO  - Tumour Biol. 2016 Jun;37(6):8139-44. doi: 10.1007/s13277-015-4715-1. Epub 2015 
      Dec 29.