PMID- 26717916
OWN - NLM
STAT- MEDLINE
DCOM- 20161223
LR  - 20190402
IS  - 0304-4920 (Print)
IS  - 0304-4920 (Linking)
VI  - 58
IP  - 6
DP  - 2015 Dec 31
TI  - Effect of Miconazole on [Ca(2)(+)]i and Cytotoxicity in ZR-75-1 Human Breast Cancer 
      Cells.
PG  - 377-84
LID - CJP.2015.BAD347 [pii]
LID - 10.4077/CJP.2015.BAD347 [doi]
AB  - The effect of the antifungal drug miconazole on Ca(2)(+) signaling in human breast 
      cancer cells is unknown. This study examined the effect of miconazole on 
      cytosolic free Ca(2)(+) concentrations ([Ca(2)(+)]i) in ZR-75-1 human breast cancer 
      cells. The Ca(2)(+)-sensitive fluorescent dye fura-2 was used to measure [Ca(2)(+)]i. 
      Miconazole induced [Ca(2)(+)]i rises concentration-dependently. The response was 
      reduced by 60% by removing extracellular Ca(2)(+). Miconazole-induced Ca(2)(+) entry was 
      abolished by the protein kinase C (PKC) inhibitor GF109203X, and nifedipine, but 
      was insensitive to econazole, SKF96365 and the protein kinase C activator phorbol 
      12-myristate 13 acetate (PMA). In Ca(2)(+)-free medium, treatment with the 
      endoplasmic reticulum Ca(2)(+) pump inhibitor 2,5-di-tert-butylhydroquinone (BHQ) or 
      thapsigargin (TG) greatly inhibited miconazole-evoked [Ca(2)(+)]i rises. Conversely, 
      treatment with miconazole abolished TG and BHQ-evoked [Ca(2)(+)]i rises. Inhibition 
      of phospholipase C (PLC) with U73122 abolished miconazole-induced [Ca(2)(+)]i rises. 
      At concentrations of 30-50 muM, micronazole killed cells in a 
      concentration-dependent manner. This cytotoxic effect was not reversed by 
      chelating cytosolic Ca(2)(+) with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic 
      acid/acetoxy methyl (BAPTA/AM). Together, in ZR-75-1 cells, miconazole induced 
      [Ca(2)(+)]i rises by evoking PLC-dependent Ca(2)(+) release from the endoplasmic 
      reticulum, and PKC-regulated nifedipine-sensitive Ca(2)(+) entry. Miconazole-caused 
      cell death was not triggered by a preceding [Ca(2)(+)]i rise.
FAU - Roan, Cherng-Jau
AU  - Roan CJ
AD  - Department of Obstetrics and Gynecology, E-DA Hospital, Kaohsiung 82445, Taiwan, 
      Republic of China.
FAU - Chou, Chiang-Ting
AU  - Chou CT
AD  - Department of Nursing, Division of Basic Medical Sciences, Chang Gung Institute 
      of Technology, Chia-Yi 61363, Taiwan, Republic of China.
AD  - Chronic Diseases and Health Promotion Research Center, Chang Gung Institute of 
      Technology, Chia-Yi 61363, Taiwan, Republic of China.
FAU - Liang, Wei-Zhe
AU  - Liang WZ
AD  - Department of Medical Education and Research, Kaohsiung Veterans General 
      Hospital, Kaohsiung 81362, Taiwan, Republic of China.
FAU - Chang, Hong-Tai
AU  - Chang HT
AD  - Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung 81362, 
      Taiwan, Republic of China.
FAU - Kuo, Daih-Huang
AU  - Kuo DH
AD  - Department of Pharmacy, Tajen University, Pingtung 90741, Taiwan, Republic of 
      China.
FAU - Kuo, Chun-Chi
AU  - Kuo CC
AD  - Department of Nursing, Tzu Hui Institute of Technology, Pingtung 92641, Taiwan, 
      Republic of China.
FAU - Chen, Fu-An
AU  - Chen FA
AD  - Department of Pharmacy, Tajen University, Pingtung 90741, Taiwan, Republic of 
      China.
FAU - Shieh, Pochuen
AU  - Shieh P
AD  - Department of Pharmacy, Tajen University, Pingtung 90741, Taiwan, Republic of 
      China.
FAU - Jan, Chung-Ren
AU  - Jan CR
AD  - Department of Pharmacy, Tajen University, Pingtung 90741, Taiwan, Republic of 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - Chin J Physiol
JT  - The Chinese journal of physiology
JID - 7804502
RN  - 7NNO0D7S5M (Miconazole)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Breast Neoplasms/*drug therapy/metabolism/pathology
MH  - Calcium/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Humans
MH  - Miconazole/*pharmacology
MH  - Type C Phospholipases/physiology
EDAT- 2016/01/01 06:00
MHDA- 2016/12/24 06:00
CRDT- 2016/01/01 06:00
PHST- 2016/01/01 06:00 [entrez]
PHST- 2016/01/01 06:00 [pubmed]
PHST- 2016/12/24 06:00 [medline]
AID - CJP.2015.BAD347 [pii]
AID - 10.4077/CJP.2015.BAD347 [doi]
PST - ppublish
SO  - Chin J Physiol. 2015 Dec 31;58(6):377-84. doi: 10.4077/CJP.2015.BAD347.