PMID- 26718409
OWN - NLM
STAT- MEDLINE
DCOM- 20160527
LR  - 20160122
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 469
IP  - 4
DP  - 2016 Jan 22
TI  - Investigation of the redox-dependent modulation of structure and dynamics in 
      human cytochrome c.
PG  - 978-84
LID - S0006-291X(15)31084-6 [pii]
LID - 10.1016/j.bbrc.2015.12.079 [doi]
AB  - Redox-dependent changes in the structure and dynamics of human cytochrome c (Cyt 
      c) were investigated by solution NMR. We found significant structural changes in 
      several regions, including residues 23-28 (loop 3), which were further 
      corroborated by chemical shift differences between the reduced and oxidized 
      states of Cyt c. These differences are essential for discriminating redox states 
      in Cyt c by cytochrome c oxidase (CcO) during electron transfer reactions. 
      Carr-Purcell-Meiboom-Gill (CPMG) relaxation dispersion experiments identified 
      that the region around His33 undergoes conformational exchanges on the mus-ms 
      timescale, indicating significant redox-dependent structural changes. Because 
      His33 is not part of the interaction site for CcO, our data suggest that the 
      dynamic properties of the region, which is far from the interaction site for CcO, 
      contribute to conformational changes during electron transfer to CcO.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Imai, Mizue
AU  - Imai M
AD  - Graduate School of Chemical Sciences and Engineering, Hokkaido University, 
      Sapporo 060-0810, Japan.
FAU - Saio, Tomohide
AU  - Saio T
AD  - Graduate School of Chemical Sciences and Engineering, Hokkaido University, 
      Sapporo 060-0810, Japan; Department of Chemistry, Faculty of Science, Hokkaido 
      University, Sapporo 060-0810, Japan.
FAU - Kumeta, Hiroyuki
AU  - Kumeta H
AD  - Faculty of Advanced Life Science, Hokkaido University, Sapporo 001-0021, Japan.
FAU - Uchida, Takeshi
AU  - Uchida T
AD  - Graduate School of Chemical Sciences and Engineering, Hokkaido University, 
      Sapporo 060-0810, Japan; Department of Chemistry, Faculty of Science, Hokkaido 
      University, Sapporo 060-0810, Japan.
FAU - Inagaki, Fuyuhiko
AU  - Inagaki F
AD  - Faculty of Advanced Life Science, Hokkaido University, Sapporo 001-0021, Japan.
FAU - Ishimori, Koichiro
AU  - Ishimori K
AD  - Graduate School of Chemical Sciences and Engineering, Hokkaido University, 
      Sapporo 060-0810, Japan; Department of Chemistry, Faculty of Science, Hokkaido 
      University, Sapporo 060-0810, Japan. Electronic address: 
      koichiro@sci.hokudai.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151222
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 9007-43-6 (Cytochromes c)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Binding Sites
MH  - Cytochromes c/*chemistry/*ultrastructure
MH  - Enzyme Activation
MH  - Humans
MH  - Kinetics
MH  - Oxidation-Reduction
MH  - Oxygen/*chemistry
MH  - Protein Binding
MH  - Protein Conformation
MH  - Structure-Activity Relationship
OTO - NOTNLM
OT  - Cytochrome c
OT  - Dynamics
OT  - Electron transfer
OT  - Solution structure
EDAT- 2016/01/01 06:00
MHDA- 2016/05/28 06:00
CRDT- 2016/01/01 06:00
PHST- 2015/12/16 00:00 [received]
PHST- 2015/12/18 00:00 [accepted]
PHST- 2016/01/01 06:00 [entrez]
PHST- 2016/01/01 06:00 [pubmed]
PHST- 2016/05/28 06:00 [medline]
AID - S0006-291X(15)31084-6 [pii]
AID - 10.1016/j.bbrc.2015.12.079 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2016 Jan 22;469(4):978-84. doi: 
      10.1016/j.bbrc.2015.12.079. Epub 2015 Dec 22.