PMID- 26722842
OWN - NLM
STAT- MEDLINE
DCOM- 20161216
LR  - 20181202
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Linking)
VI  - 25
IP  - 4
DP  - 2016
TI  - Induction of Endothelial Phenotype From Wharton's Jelly-Derived MSCs and 
      Comparison of Their Vasoprotective and Neuroprotective Potential With Primary 
      WJ-MSCs in CA1 Hippocampal Region Ex Vivo.
PG  - 715-27
LID - 10.3727/096368915X690369 [doi]
AB  - Ischemic stroke results in violent impairment of tissue homeostasis leading to 
      severe perturbation within the neurovascular unit (NVU) during the recovery 
      period. The aim of this study was to assess the potential of mesenchymal stem 
      cells (MSCs) originating from Wharton's jelly (WJ) to differentiate into 
      functionally competent cells of endothelial lineage (WJ-EPCs). The protective 
      effect(s) of either primary WJ-MSCs or induced WJ-EPCs was investigated and 
      compared after oxygen-glucose deprivation (OGD) of hippocampal organotypic slices 
      (OHC) in the indirect coculture model. WJ-MSCs, primed in EGM-2 (Lonza commercial 
      medium) under 5% O2, acquired cobblestone endothelial-like morphology, formed 
      capillary-like structures and actively took up DiI-Ac-LDL. Both cell types 
      (WJ-MSCs and WJ-EPCs) were positive for CD73, CD90, CD105, VEGFR-2, and VEGF, but 
      only endothelial-like culture expressed vWF and PECAM-1 markers at significant 
      levels. In the presence of either WJ-MSCs or WJ-EPCs in the compartment below 
      OGD-injured slices, cell death and vascular atrophy in the hypoxia-sensitive CA1 
      region were substantially decreased. This suggests that a paracrine mechanism may 
      mediate WJ-MSC- and WJ-EPC-dependent protection. Thus, finally, we estimated 
      secretion of the neuro/angio/immunomodulatory molecules IL-6, TGF-beta1, and VEGF by 
      these cell cultures. We have found that release of TGF-beta1 and IL-6 was TLR ligand 
      [LPS and Poly(I:C)] concentration dependent and stronger in WJ-EPC than WJ-MSC 
      cultures. Simultaneously, the uneven pattern of TLR receptors and modulatory 
      cytokine gene expression was confirmed also on qRT-PCR level, but no significant 
      differences were noticed between WJ-EPC and primary WJ-MSC cultures.
FAU - Obtulowicz, Patrycja
AU  - Obtulowicz P
AD  - Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.
FAU - Lech, Wioletta
AU  - Lech W
FAU - Strojek, Lukasz
AU  - Strojek L
FAU - Sarnowska, Anna
AU  - Sarnowska A
FAU - Domanska-Janik, Krystyna
AU  - Domanska-Janik K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151231
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (Antigens, Differentiation)
SB  - IM
MH  - Animals
MH  - Antigens, Differentiation/*biosynthesis
MH  - CA1 Region, Hippocampal/*metabolism
MH  - Coculture Techniques
MH  - Female
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Rats
MH  - Rats, Wistar
EDAT- 2016/01/02 06:00
MHDA- 2016/12/17 06:00
CRDT- 2016/01/02 06:00
PHST- 2016/01/02 06:00 [entrez]
PHST- 2016/01/02 06:00 [pubmed]
PHST- 2016/12/17 06:00 [medline]
AID - content-CT-1517_Obtulowicz_et_al [pii]
AID - 10.3727/096368915X690369 [doi]
PST - ppublish
SO  - Cell Transplant. 2016;25(4):715-27. doi: 10.3727/096368915X690369. Epub 2015 Dec 
      31.