PMID- 26723394 OWN - NLM STAT- MEDLINE DCOM- 20161114 LR - 20181202 IS - 1557-8534 (Electronic) IS - 1547-3287 (Linking) VI - 25 IP - 5 DP - 2016 Mar 1 TI - miR-26b-3p Regulates Human Umbilical Cord-Derived Mesenchymal Stem Cell Proliferation by Targeting Estrogen Receptor. PG - 415-26 LID - 10.1089/scd.2015.0267 [doi] AB - Human umbilical cord-derived mesenchymal stem cells (hUC-MSC) have been considered as promising candidates for cell-based regeneration medicine. However, the application was limited to its poor in vitro proliferation ability against the huge demand of cells. MicroRNA plays important roles in the regulation of cell proliferation, apoptosis, and differentiation. The objective of this study is to explore the roles of miRNAs in regulating the in vitro proliferation of hUC-MSC and unveil their possible mechanism. In this study, we found that miR-26b-3p was significantly upregulated during serial in vitro passage of hUC-MSC and was correlated with cellular senescence and cell cycle genes. The overexpression of miR-26b-3p greatly inhibited the proliferation of hUC-MSC in vitro, which is indicated by 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay, cell cycle, and cell growth curve analyses. miR-26b-3p suppression partly rescued this phenotype by maintaining its proliferation ability in vitro. For mechanism studies, we predicted and validated that miR-26b-3p suppresses estrogen receptor 1 (ESR1) expression by directly binding to the coding sequence (CDS) region of its message RNA (mRNA), thus subsequently changing the expression of its downstream effector Cyclin D1. In conclusion, we found that miR-26b-3p played an important role in the regulation of hUC-MSC proliferation in vitro by targeting the ESR-CCND1 pathway. FAU - Wang, Qiaoling AU - Wang Q AD - 1 Translational Medicine Center, Second Military Medical University , Shanghai, People's Republic of China . AD - 2 Department of Histology and Embryology, Research Center of Developmental Biology, Second Military Medical University , Shanghai, People's Republic of China . FAU - Xu, Chen AU - Xu C AD - 1 Translational Medicine Center, Second Military Medical University , Shanghai, People's Republic of China . AD - 2 Department of Histology and Embryology, Research Center of Developmental Biology, Second Military Medical University , Shanghai, People's Republic of China . AD - 3 Department of Spinal Surgery, Changzheng Hospital, Second Military Medical University , Shanghai, People's Republic of China . FAU - Zhao, Yunpeng AU - Zhao Y AD - 1 Translational Medicine Center, Second Military Medical University , Shanghai, People's Republic of China . AD - 2 Department of Histology and Embryology, Research Center of Developmental Biology, Second Military Medical University , Shanghai, People's Republic of China . FAU - Xu, Zhenyu AU - Xu Z AD - 1 Translational Medicine Center, Second Military Medical University , Shanghai, People's Republic of China . AD - 2 Department of Histology and Embryology, Research Center of Developmental Biology, Second Military Medical University , Shanghai, People's Republic of China . FAU - Zhang, Yan AU - Zhang Y AD - 1 Translational Medicine Center, Second Military Medical University , Shanghai, People's Republic of China . AD - 2 Department of Histology and Embryology, Research Center of Developmental Biology, Second Military Medical University , Shanghai, People's Republic of China . FAU - Jiang, Junfeng AU - Jiang J AD - 1 Translational Medicine Center, Second Military Medical University , Shanghai, People's Republic of China . AD - 2 Department of Histology and Embryology, Research Center of Developmental Biology, Second Military Medical University , Shanghai, People's Republic of China . FAU - Yan, Binghao AU - Yan B AD - 1 Translational Medicine Center, Second Military Medical University , Shanghai, People's Republic of China . AD - 2 Department of Histology and Embryology, Research Center of Developmental Biology, Second Military Medical University , Shanghai, People's Republic of China . FAU - Gu, Daolan AU - Gu D AD - 1 Translational Medicine Center, Second Military Medical University , Shanghai, People's Republic of China . AD - 2 Department of Histology and Embryology, Research Center of Developmental Biology, Second Military Medical University , Shanghai, People's Republic of China . FAU - Wu, Minjuan AU - Wu M AD - 1 Translational Medicine Center, Second Military Medical University , Shanghai, People's Republic of China . FAU - Wang, Yue AU - Wang Y AD - 1 Translational Medicine Center, Second Military Medical University , Shanghai, People's Republic of China . AD - 2 Department of Histology and Embryology, Research Center of Developmental Biology, Second Military Medical University , Shanghai, People's Republic of China . FAU - Liu, Houqi AU - Liu H AD - 1 Translational Medicine Center, Second Military Medical University , Shanghai, People's Republic of China . AD - 2 Department of Histology and Embryology, Research Center of Developmental Biology, Second Military Medical University , Shanghai, People's Republic of China . LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160208 PL - United States TA - Stem Cells Dev JT - Stem cells and development JID - 101197107 RN - 0 (CCND1 protein, human) RN - 0 (MIRN26A microRNA, human) RN - 0 (MicroRNAs) RN - 0 (RNA, Messenger) RN - 0 (Receptors, Estrogen) RN - 136601-57-5 (Cyclin D1) SB - IM MH - Adipogenesis MH - Base Sequence MH - Cell Differentiation MH - Cell Proliferation MH - Cellular Senescence/genetics MH - Cyclin D1/metabolism MH - Gene Expression Regulation MH - Humans MH - Mesenchymal Stem Cells/*cytology/*metabolism MH - MicroRNAs/genetics/metabolism MH - Molecular Sequence Data MH - RNA, Messenger/genetics/metabolism MH - Receptors, Estrogen/*genetics/metabolism MH - Umbilical Cord/*cytology EDAT- 2016/01/03 06:00 MHDA- 2016/11/15 06:00 CRDT- 2016/01/03 06:00 PHST- 2016/01/03 06:00 [entrez] PHST- 2016/01/03 06:00 [pubmed] PHST- 2016/11/15 06:00 [medline] AID - 10.1089/scd.2015.0267 [doi] PST - ppublish SO - Stem Cells Dev. 2016 Mar 1;25(5):415-26. doi: 10.1089/scd.2015.0267. Epub 2016 Feb 8.