PMID- 26723494
OWN - NLM
STAT- MEDLINE
DCOM- 20161101
LR  - 20161230
IS  - 1096-0309 (Electronic)
IS  - 0003-2697 (Linking)
VI  - 497
DP  - 2016 Mar 15
TI  - Miniaturized and direct spectrophotometric multi-sample analysis of trace metals 
      in natural waters.
PG  - 18-23
LID - S0003-2697(15)00570-9 [pii]
LID - 10.1016/j.ab.2015.11.025 [doi]
AB  - Trends in the analysis of trace metals in natural waters are mainly based on the 
      development of sample treatment methods to isolate and pre-concentrate the metal 
      from the matrix in a simpler extract for further instrumental analysis. However, 
      direct analysis is often possible using more accessible techniques such as 
      spectrophotometry. In this case a proper ligand is required to form a complex 
      that absorbs radiation in the ultraviolet-visible (UV-Vis) spectrum. In this 
      sense, the hydrazone derivative, di-2-pyridylketone benzoylhydrazone (dPKBH), 
      forms complexes with copper (Cu) and vanadium (V) that absorb light at 370 and 
      395 nm, respectively. Although spectrophotometric methods are considered as time- 
      and reagent-consuming, this work focused on its miniaturization by reducing the 
      volume of sample as well as time and cost of analysis. In both methods, a 
      micro-amount of sample is placed into a microplate reader with a capacity for 96 
      samples, which can be analyzed in times ranging from 5 to 10 min. The proposed 
      methods have been optimized using a Box-Behnken design of experiments. For Cu 
      determination, concentration of phosphate buffer solution at pH 8.33, masking 
      agents (ammonium fluoride and sodium citrate), and dPKBH were optimized. For V 
      analysis, sample (pH 4.5) was obtained using acetic acid/sodium acetate buffer, 
      and masking agents were ammonium fluoride and 1,2-cyclohexanediaminetetraacetic 
      acid. Under optimal conditions, both methods were applied to the analysis of 
      certified reference materials TMDA-62 (lake water), LGC-6016 (estuarine water), 
      and LGC-6019 (river water). In all cases, results proved the accuracy of the 
      method.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Albendin, Gemma
AU  - Albendin G
AD  - Laboratory of Toxicology Faculty of Marine and Environmental Sciences, 
      Universidad de Cadiz, Cadiz 11510, Spain.
FAU - Lopez-Lopez, Jose A
AU  - Lopez-Lopez JA
AD  - Department of Analytical Chemistry, Faculty of Marine and Environmenal Sciences, 
      Universidad de Cadiz, Cadiz 11510, Spain. Electronic address: 
      joseantonio.lopezlopez@uca.es.
FAU - Pinto, Juan J
AU  - Pinto JJ
AD  - Department of Analytical Chemistry, Faculty of Marine and Environmenal Sciences, 
      Universidad de Cadiz, Cadiz 11510, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151223
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Hydrazones)
RN  - 0 (Indicators and Reagents)
RN  - 00J9J9XKDE (Vanadium)
RN  - 789U1901C5 (Copper)
SB  - IM
MH  - Copper/*analysis
MH  - Environmental Monitoring/methods
MH  - Fresh Water/*analysis
MH  - Hydrazones/chemistry
MH  - Indicators and Reagents
MH  - Spectrophotometry, Ultraviolet/*methods
MH  - Vanadium/*analysis
OTO - NOTNLM
OT  - Box-Behnken
OT  - Cu
OT  - Microanalysis
OT  - Trace metals
OT  - V
OT  - dPKBH
EDAT- 2016/01/03 06:00
MHDA- 2016/11/02 06:00
CRDT- 2016/01/03 06:00
PHST- 2015/07/16 00:00 [received]
PHST- 2015/11/04 00:00 [revised]
PHST- 2015/11/28 00:00 [accepted]
PHST- 2016/01/03 06:00 [entrez]
PHST- 2016/01/03 06:00 [pubmed]
PHST- 2016/11/02 06:00 [medline]
AID - S0003-2697(15)00570-9 [pii]
AID - 10.1016/j.ab.2015.11.025 [doi]
PST - ppublish
SO  - Anal Biochem. 2016 Mar 15;497:18-23. doi: 10.1016/j.ab.2015.11.025. Epub 2015 Dec 
      23.