PMID- 26724741
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20170911
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 92
DP  - 2016 Mar
TI  - Porphyromonas gingivalis infection modifies oral microcirculation and aortic 
      vascular function in the stroke-prone spontaneously hypertensive rat (SHRSP).
PG  - 36-42
LID - S0882-4010(15)30175-3 [pii]
LID - 10.1016/j.micpath.2015.12.009 [doi]
AB  - The functional modulation of vascular endothelial cells associated with stroke 
      and periodontal disease has not yet been clarified. The objective of this study 
      is to analyze the vascular endothelial function of periodontitis and stroke 
      animal models. We examined endothelial function and gingival blood flow in oral 
      microcirculation in vivo and measured the isometric tension in vitro of the aorta 
      in animal models for lifestyle-related diseases, such as periodontitis and 
      stroke. Gingival reactive hyperemia (GRH) was measured using laser Doppler 
      flowmetry. Wistar Kyoto rats (WKY) were used as control animals; Porphyromonas 
      gingivalis (P. gingivalis) infected WKY (WKY + Pg) as the periodontitis model; 
      stroke-prone spontaneously hypertensive rat (SHRSP) as the stroke model; and a 
      final group consisting of P. gingivalis infected SHRSP (SHRSP + Pg). Furthermore, 
      for each group, the relaxation of descending aortic ring preparations was 
      measured using a force transducer. The GRH was estimated by maximum response 
      (peak), time taken for the maximum response to fall to one half (T1/2), and 
      increased total amount of blood flow (mass). The relative change in T1/2 and mass 
      increased in SHRSP + Pg compared to WKY. However, mass significantly increased in 
      WKY (758.59 +/- 88.21 ml/min/100 g s to 1755.55 +/- 226.10 ml/min/100 g s) and SHRSP 
      (1214.87 +/- 141.61 ml/min/100 g s to 2674.32 +/- 675.48 ml/min/100 g s) after 
      treatment with acetylcholine. In addition, T1/2 and mass significantly increased 
      in WKY + Pg (624.18 +/- 96.36 ml/min/100 g s to 2629.90 +/- 612.01 ml/min/100 g s) 
      and SHRSP + Pg (1116.36 +/- 206.24 ml/min/100 g s to 1952.76 +/- 217.39 ml/min/100 g 
      s) after treatment with nitroglycerin. Furthermore, the endothelium-dependent 
      relaxation of ring preparations, evoked by acetylcholine, was attenuated in SHRSP 
      compared with WKY, but not in SHRSP + Pg. This attenuation effect in SHRSP could 
      be prevented by superoxide dismutase pretreatment. Our results suggest altered 
      endothelial function may occur in gingival tissue in animal models experiencing 
      both periodontitis and stroke. Therefore, these results indicate the disruption 
      of vascular function in oral microcirculation may be caused by the interaction 
      between the oxidative stress induced by periodontitis and nitric oxide in 
      periodontitis, similar to the interactions present in stroke cases.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Funaki, Seiko
AU  - Funaki S
AD  - Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental 
      University, 82 Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan.
FAU - Tokutomi, Fumiaki
AU  - Tokutomi F
AD  - Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental 
      University, 82 Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan.
FAU - Wada-Takahashi, Satoko
AU  - Wada-Takahashi S
AD  - Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental 
      University, 82 Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan. Electronic 
      address: s.takahashi@kdu.ac.jp.
FAU - Yoshino, Fumihiko
AU  - Yoshino F
AD  - Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental 
      University, 82 Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan.
FAU - Yoshida, Ayaka
AU  - Yoshida A
AD  - Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental 
      University, 82 Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan.
FAU - Maehata, Yojiro
AU  - Maehata Y
AD  - Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental 
      University, 82 Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan.
FAU - Miyamoto, Chihiro
AU  - Miyamoto C
AD  - Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental 
      University, 82 Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan.
FAU - Toyama, Toshizo
AU  - Toyama T
AD  - Division of Microbiology, Department of Infection Control, Graduate School of 
      Dentistry, Kanagawa Dental University, Kanagawa, Japan.
FAU - Sato, Takenori
AU  - Sato T
AD  - Division of Microbiology, Department of Infection Control, Graduate School of 
      Dentistry, Kanagawa Dental University, Kanagawa, Japan.
FAU - Hamada, Nobushiro
AU  - Hamada N
AD  - Division of Microbiology, Department of Infection Control, Graduate School of 
      Dentistry, Kanagawa Dental University, Kanagawa, Japan.
FAU - Lee, Masaichi Chang-Il
AU  - Lee MC
AD  - Yokosuka-Shonan Disaster Health Emergency Research Center & ESR Laboratories, 
      Kanagawa Dental University, 82 Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan.
FAU - Takahashi, Shun-Suke
AU  - Takahashi SS
AD  - Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental 
      University, 82 Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan. Electronic 
      address: takahashi.shunsuke@kdu.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151225
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
SB  - IM
MH  - Animals
MH  - Aorta/*physiopathology
MH  - Bacteroidaceae Infections/*microbiology/*physiopathology
MH  - Blood Pressure
MH  - Disease Models, Animal
MH  - Hyperemia/etiology
MH  - Male
MH  - *Microcirculation
MH  - Periodontitis/*microbiology/*physiopathology
MH  - *Porphyromonas gingivalis
MH  - Rats
MH  - Rats, Inbred SHR
MH  - Regional Blood Flow
MH  - Stroke/*etiology/physiopathology
OTO - NOTNLM
OT  - Gingival blood flow
OT  - Gingival reactive hyperemia
OT  - Laser Doppler flowmetry
OT  - Periodontitis
OT  - Reactive oxygen species (ROS)
OT  - Stroke-prone spontaneously hypertensive rat (SHRSP)
EDAT- 2016/01/03 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/01/03 06:00
PHST- 2015/10/31 00:00 [received]
PHST- 2015/12/15 00:00 [revised]
PHST- 2015/12/22 00:00 [accepted]
PHST- 2016/01/03 06:00 [entrez]
PHST- 2016/01/03 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - S0882-4010(15)30175-3 [pii]
AID - 10.1016/j.micpath.2015.12.009 [doi]
PST - ppublish
SO  - Microb Pathog. 2016 Mar;92:36-42. doi: 10.1016/j.micpath.2015.12.009. Epub 2015 
      Dec 25.